Pico Signal Western ECL Substrate FAQs

1. What is the recommended dilution range for Hypersensitive ECL chemiluminescence Kit?

Primary antibody recommended dilution range is 0.2 to 1.0 µg/mL (1:1000 to 1:5000 dilution from a 1 mg/mL stock). Secondary antibody recommended dilution range is 10 to 50 ng/mL (1:20,000 to 1:100,000 dilution from 1 mg/mL stock).

2. What is the sensitivity of Hypersensitive ECL chemiluminescence Kit?

Hypersensitive ECL chemiluminescence Kit enables detection of low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane.