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Lentivirus vector system - Introduction

GeneMedi's lentivirus Vector System, also named the lentivirus expression system or lentivirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi), gene editing and stable cellline development. You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System.

The Genemedi lentivirus vector system including 3 lentivirus packaging plasmids: the lentivirus expression plasmids with different promoters and tags, an envelope protein VSV-G expressing plasmid pMD2G and a packaging plasmid pSPAX2 expressing Gag-pol and Tat.

Figure 1: Workflow of lentivirus packaging

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GeneMedi has developed a variety of lentivirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's lentivirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA lentivirus expression vector.



Lentivirus vector system


Product Name Contents Price(In USD) Qty (Quantity) Sum(In USD)
Lentivirus vector system 1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52)
(Expression vector refers only to B52, if you need other expression vector, please check the table below)

2025
PMD2G Cat No.: P-ALV-C01; size: 5ug 650
pSPAX2 Cat No.: P-ALV-C02; size: 5ug 650
Shipping Cost: 169.00
Total:

Lentivirus expression vectors (lentivirus expression plasmids)


Cat.No Expression Name of Vectors Promoter Reporter Cre-dependent TAG (unfusion) Price (in USD) Order
P-ALV-B01OverexpressionpCDH-MSCV-MCS-EF1-copGFP-T2A-PuromycinMSCVcopGFPPuromycinNULL998Picture loading failed.
P-ALV-B02OverexpressionpGMLV-CMV-Circ-MCS-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycinNULL1598Picture loading failed.
P-ALV-B03OverexpressionpGMLV-CMV-MCS-3flag-EF1-fLUC-T2A-PuromycinCMVLuciferasePuromycin3FLAG(C-terminal)1288Picture loading failed.
P-ALV-B04OverexpressionpGMLV-CMV-MCS-EF1-fLUC-T2A-PuromycinCMVLuciferasePuromycinNULL1288Picture loading failed.
P-ALV-B05OverexpressionpGMLV-CMV-MCS-EF1-mCherry-T2A-PuromycinCMVmCherryPuromycinNULL1084Picture loading failed.
P-ALV-B06OverexpressionpGMLV-CMV-MCS-3flag-EF1-mCherry-T2A-PuromycinCMVmCherryPuromycin3FLAG(C-terminal)1084Picture loading failed.
P-ALV-B07OverexpressionpGMLV-CMV-MCS-EF1-PuromycinCMVNullPuromycinNULL1084Picture loading failed.
P-ALV-B08OverexpressionpGMLV-CMV-MCS-3flag-EF1-PuromycinCMVNullPuromycin3FLAG(C-terminal)1084Picture loading failed.
P-ALV-B09OverexpressionpGMLV-CMV-MCS-3flag-T2A-ZsGreenCMVZsGreenNull3FLAG(C-terminal)998Picture loading failed.
P-ALV-B10OverexpressionpGMLV-CMV-MCS-T2A-ZsGreenCMVZsGreenNullNULL998Picture loading failed.
P-ALV-B11OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycin3FLAG(C-terminal)998Picture loading failed.
P-ALV-B12OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B13OverexpressionpGMLV-CMV-MCS-T2A-PuromycinCMVNullPuromycinNULL1084Picture loading failed.
P-ALV-B14OverexpressionpGMLV-CMV-MCS-dsRed-PGK-PuromycinCMVdsRedPuromycinNULL1084Picture loading failed.
P-ALV-B15OverexpressionpGMLV-CMV-MCS-IRES-PuromycinCMVNullPuromycinNULL1084Picture loading failed.
P-ALV-B16OverexpressionpGMLV-CMV-MCS-IRES-ZsGreenCMVZsGreenNullNULL998Picture loading failed.
P-ALV-B17OverexpressionpGMLV-CMV-MCS-PGK-ZsGreenCMVZsGreenNullNULL998Picture loading failed.
P-ALV-B18OverexpressionpGMLV-CMV-MCS-PGK-Puromycin-IRES-ZsGreenCMVZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B19shRNA for RNAipGMLV-U6-MCS-PGK-PuromycinU6NullPuromycinNULL1084Picture loading failed.
P-ALV-B20shRNA for RNAipGMLV-U6-MCS-CMV-ZsGreenU6ZsGreenNullNULL998Picture loading failed.
P-ALV-B21shRNA for RNAipGMLV-U6-MCS-CMV-ZsGreen-PGK-PuromycinU6ZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B22OverexpressionpGMLV-EM7-MCS-Ubc-rtTA3-PuromycinEM7NullPuromycinNULL1084Picture loading failed.
P-ALV-B23OverexpressionpGMLV-PGK-Puromycin-Tnl-EGFPPGKEGFPPuromycinNULL998Picture loading failed.
P-ALV-B24OverexpressionpGMLV-CMV-MCS-PGK-RFPCMVRFPNullNULL1084Picture loading failed.
P-ALV-B25OverexpressionpGMLV-CMV-MCS-PGK-Z418CMVNullZ418NULL1084Picture loading failed.
P-ALV-B26shRNA for RNAipGMLV-U6-MCS-PGK-BSDU6NullBlasticidinNULL1084Picture loading failed.
P-ALV-B27OverexpressionpGMLV-TetOn-SV40-Puromycin-TRE3GS-MCSTRE3GSNullPuromycinNULL1084Picture loading failed.
P-ALV-B28shRNA for RNAipGMLV-U6-MCS-hPGK-PuromycinU6NullPuromycinNULL1084Picture loading failed.
P-ALV-B29OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-LucCMVRFP/LuciferaseNullNULL2088Picture loading failed.
P-ALV-B30OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-PuromycinCMVRFPPuromycinNULL1084Picture loading failed.
P-ALV-B31OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-LucCMVZsGreen/LuciferaseNullNULL1988Picture loading failed.
P-ALV-B32shRNA for RNAipGMLV-U6-MCS-EF1-Luc-T2A-PuromycinU6LuciferasePuromycinNULL1288Picture loading failed.
P-ALV-B33shRNA for RNAipGMLV-U6-MCS-EF1-RFP-T2A-LucU6RFP/LuciferaseNullNULL2088Picture loading failed.
P-ALV-B34shRNA for RNAipGMLV-U6-MCS-EF1-RFP-T2A-PuromycinU6RFPPuromycinNULL1084Picture loading failed.
P-ALV-B35shRNA for RNAipGMLV-U6-MCS-EF1-ZsGreen-T2A-LucU6ZsGreen/LuciferaseNullNULL1988Picture loading failed.
P-ALV-B36shRNA for RNAipGMLV-U6-MCS-EF1-ZsGreen-T2A-BSDU6ZsGreenBlasticidinNULL1188Picture loading failed.
P-ALV-B37OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-BSDCMVZsGreenBlasticidinNULL1188Picture loading failed.
P-ALV-B38shRNA for RNAipGMLV-U6-MCS-EF1-fLuc-T2A-mCherryU6mCherry/LuciferaseNullNULL2088Picture loading failed.
P-ALV-B39OverexpressionpGMLV-CMV-MCS-EF1-fLuc-T2A-mcherryCMVmCherry/LuciferaseNullNULL2088Picture loading failed.
P-ALV-B40shRNA for RNAipGMLV-U6-MCS-EF1-fLuc-T2A-BSDU6LuciferaseBlasticidinNULL1988Picture loading failed.
P-ALV-B41shRNA for RNAipGMLV-U6-MCS-PGK-Z418U6NullZ418NULL1084Picture loading failed.
P-ALV-B42OverexpressionpGMLV-CMV-MCS-fLUC-EF1-ZsGreen-T2A-PuromycinCMVZsGreen/LuciferasePuromycinNULL2888Picture loading failed.
P-ALV-B43OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin(I)CMVZsGreenPuromycin3FLAG(C-terminal)998Picture loading failed.
P-ALV-B44OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-fLUC(I)CMVZsGreen/LuciferaseNullNULL1988Picture loading failed.
P-ALV-B45shRNA for RNAipGMLV-U6-MCS-CMV-mCherry-P2A-BSDU6mCherryBlasticidinNULL1388Picture loading failed.
P-ALV-B46shRNA for RNAipGMLV-U6-MCS-CMV-EGFP-P2A-BSDU6EGFPBlasticidinNULL1188Picture loading failed.
P-ALV-B47OverexpressionpGMLV-CMV-DIO-ZsGreen-P2A-MCS-DIO-EF1-PuromycinCMVZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B48OverexpressionpGMLV-CMV-MCS-P2A-ZsGreenCMVZsGreenNullNULL998Picture loading failed.
P-ALV-B49gRNApGMLV-U6-gRNA-EF1-PuromycinU6NullPuromycinNULL1084Picture loading failed.
P-ALV-B50cas9/gRNApGMLV-U6-gRNA-EF1-CAS9-PuromycinU6NullPuromycin1FLAG1084Picture loading failed.
P-ALV-B51shRNA for RNAipGMLV-U6-MCS-EF1-mCherry-T2A-PuromycinU6mCherryPuromycinNULL1084Picture loading failed.
P-ALV-B52OverexpressionpGMLV-CMV-MCS-EF1-ZsGreenCMVZsGreenNullNULL998Picture loading failed.
P-ALV-B53OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreenCMVZsGreenNull3FLAG(C-terminal)998Picture loading failed.
P-ALV-B54OverexpressionpCDH-MSCV-MCS-3flag-EF1-copGFP-T2A-PuromycinMSCVcopGFPPuromycin3FLAG(C-terminal)998Picture loading failed.
P-ALV-B55gRNApGMLV-U6-gRNA-EF1-ZsGreenU6ZsGreenNullNULL998Picture loading failed.
P-ALV-B56OverexpressionpGMLV-CMV-MCS-EF1-fLUC-T2A-BSDCMVLuciferaseBlasticidinNULL1988Picture loading failed.
P-ALV-B57OverexpressionpGMLV-CMV-MCS-EF1-Cas9-P2A-BSDCMVNullBlasticidinNULL1084Picture loading failed.
P-ALV-B58OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-HygroCMVRFPhygroNULL1388Picture loading failed.
P-ALV-B59shRNA for RNAipGMLV-U6-CMV-RFP-P2A-BSD-NU6RFPBlasticidinNULL1388Picture loading failed.
P-ALV-B60OverexpressionpGMLV-CMV-EF1-ZsGreen-P2A-BSDCMVZsGreenBlasticidinNULL1188Picture loading failed.
P-ALV-B61OverexpressionpGMLV-CMV-MCS-T2A-mCherryCMVmCherryNullNULL1084Picture loading failed.
P-ALV-B62shRNA for RNAipGMLV-U6-CMV-EGFP-P2A-BSDU6EGFPBlasticidinNULL1188Picture loading failed.
P-ALV-B63OverexpressionpGMLV-DIO-ZsGreen-P2A-MCS-DIO-PuromycinCMVZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B64OverexpressionpGMLV-CMV-EF1-ZsGreen-P2A-PuromycinCMVZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B65OverexpressionpGMLV-CMV-P2A-ZsGreen-P2A-PuromycinCMVZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B66OverexpressionpGMLV-MCS-EF1-ZsGreen-T2A-PuromycinNullZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B67LC3pGMLV-CMV-EGFP-LC3-T2A-PuromycinCMVEGFPPuromycinNULL998Picture loading failed.
P-ALV-B68LC3pGMLV-CMV-mCherry-EGFP-LC3-T2A-PuromycinCMVEGFP/mCherryPuromycinNULL2888Picture loading failed.
P-ALV-B69cas9/gRNApGMLV-U6-scramble-EF1-cas9-PuromycinU6NullPuromycinNULL1084Picture loading failed.
P-ALV-B70cas9/gRNApGMLV-U6-scramble-CMV-Cas9-T2A-ZsGreenU6ZsGreenNullNULL998Picture loading failed.
P-ALV-B71cas9/gRNApGMLV-U6-scramble-EF1-Cas9-P2A-ZsGreenU6ZsGreenNullNULL998Picture loading failed.
P-ALV-B72OverexpressionpGMLV-CMV-MCS-EF1-Z418CMVNullZ418NULL1084Picture loading failed.
P-ALV-B73OverexpressionpGMLV-CMV-MCS-3flag-EF1-mCherry-P2A-BSDCMVmCherryBlasticidin3FLAG(C-terminal)1388Picture loading failed.
P-ALV-B74shRNA for RNAipGMLV-U6-MCS-PGK-Z418(E)U6NullZ418NULL1084Picture loading failed.
P-ALV-B75shRNA for RNAipGMLV-U6-gRNA-PGK-BSDU6NullBlasticidinNULL1084Picture loading failed.
P-ALV-B76OverexpressionpGMLV-EF1-T2A-MCS-ZsGreenEF1ZsGreenNullNULL998Picture loading failed.
P-ALV-B77cas9/gRNApGMLV-U6-gRNA-EF1-mCherry-BSDU6mCherryBlasticidinNULL1388Picture loading failed.
P-ALV-B78OverexpressionpGMLV-CMV-DIO-EF1-PuromycinCMVNullPuromycinNULL1084Picture loading failed.
P-ALV-B79OverexpressionpGMLV-CMV-Circ-MCS-EF1-ZsGreenCMVZsGreenNullNULL1598Picture loading failed.
P-ALV-B80OverexpressionpGMLV-CMV-cre-3flag-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycin3FLAG(C-terminal)998Picture loading failed.
P-ALV-B81OverexpressionpGMLV-EF1-MCS-CMV-PuromycinEF1NullPuromycinNULL1084Picture loading failed.
P-ALV-B82OverexpressionpGMLV-EF1-MCS-CMV-ZsGreenEF1ZsGreenNullNULL998Picture loading failed.
P-ALV-B83OverexpressionpGMLV-EF1-MCS-CMV-ZsGreen-T2A-PuromycinEF1ZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B84OverexpressionpGMLV-EF1-MCS-P2A-ZsGreen-P2A-PuromycinEF1ZsGreenPuromycinNULL998Picture loading failed.
P-ALV-B85OverexpressionpGMLV-CMV-MCS-3flag-EF1-mcherryCMVmCherryNull3FLAG(C-terminal)1084Picture loading failed.
P-ALV-B86OverexpressionpGMLV-CMV-MCS-3flag-EF1-eRFP-T2A-PuromycinCMVeRFPPuromycin3FLAG(C-terminal)2880Picture loading failed.
P-ALV-B87shRNA for RNAipGMLV-U6-MCS-EF1-eRFP-T2A-PuromycinU6eRFPPuromycinNULL2880Picture loading failed.
P-ALV-B88OverexpressionpGMLV-CMV-eRFP-EGFP-LC3-T2A -PuromycinCMVeRFP/EGFPPuromycinNULL2880Picture loading failed.
P-ALV-B89OverexpressionpGMLV-CMV-circ-EF1-copGFP-PuromycinCMVcopGFPPuromycinNULL1598Picture loading failed.
P-ALV-B90OverexpressionpGMLV-CMV-LUC-EF1-eRFP-T2A-PuromycinCMVeRFP/LuciferasePuromycinNULL2880Picture loading failed.
P-ALV-B91OverexpressionpGMLV-CMV-circ-EF1-ZsGreen-T2A-Puromycin-NCMVZsGreenPuromycinNULL1598Picture loading failed.



Helper plasmids of lentivirus packaging

PMD2G: an envelope protein VSV-G expressing plasmid

Figure 1: Map and sequence of pMD2G

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Picture loading failed. Download the detail information of pMD2G here and read in Snapgene Software.

Click here to download SnapGene Viewer



pSPAX2: lentivirus packaging plasmids expressing Gag-pol and Tat

Figure 2: Map and sequence of pSPAX2

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Picture loading failed. Download the detail information of pSPAX2 here and read in Snapgene Software.

Click here to download SnapGene Viewer