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Lentivirus vector system - Introduction

GeneMedi's lentivirus Vector System, also named the lentivirus expression system or lentivirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi), gene editing and stable cellline development. You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System.

The Genemedi lentivirus vector system including 3 lentivirus packaging plasmids: the lentivirus expression plasmids with different promoters and tags, an envelope protein VSV-G expressing plasmid pMD2G and a packaging plasmid pSPAX2 expressing Gag-pol and Tat.

Figure 1: Workflow of lentivirus packaging

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GeneMedi has developed a variety of lentivirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's lentivirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA lentivirus expression vector.



Lentivirus vector system


Product Name Organization Type Price(In USD) Qty (Quantity) Sum(In USD)
Lentivirus vector system
Cat.No.:P-PK23
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52)
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
Academic 2430
PMD2G
Cat No.: P-ALV-C01; size: 5ug
Academic 780
pSPAX2
Cat No.: P-ALV-C02; size: 5ug
Academic 780
Lentivirus vector system
Cat.No.:P-PK23
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52)
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
Industry 4860
PMD2G
Cat No.: P-ALV-C01; size: 5ug
Industry 1560
pSPAX2
Cat No.: P-ALV-C02; size: 5ug
Industry 1560
Shipping Cost: 169.00
Total:

Lentivirus expression vectors (lentivirus expression plasmids)


Cat.No Expression Name of Vectors Promoter Reporter Cre-dependent TAG (unfusion) Price (in USD) Order
P-ALV-B01OverexpressionpCDH-MSCV-MCS-EF1-copGFP-T2A-PuromycinMSCVcopGFPPuromycinNULLAcademic: 1198
Industry: 2395
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P-ALV-B02OverexpressionpGMLV-CMV-Circ-MCS-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1918
Industry: 3836
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P-ALV-B03OverexpressionpGMLV-CMV-MCS-3flag-EF1-fLUC-T2A-PuromycinCMVLuciferasePuromycin3FLAG(C-terminal)Academic: 1546
Industry: 3092
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P-ALV-B04OverexpressionpGMLV-CMV-MCS-EF1-fLUC-T2A-PuromycinCMVLuciferasePuromycinNULLAcademic: 1546
Industry: 3092
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P-ALV-B05OverexpressionpGMLV-CMV-MCS-EF1-mCherry-T2A-PuromycinCMVmCherryPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B06OverexpressionpGMLV-CMV-MCS-3flag-EF1-mCherry-T2A-PuromycinCMVmCherryPuromycin3FLAG(C-terminal)Academic: 1301
Industry: 2602
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P-ALV-B07OverexpressionpGMLV-CMV-MCS-EF1-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B08OverexpressionpGMLV-CMV-MCS-3flag-EF1-PuromycinCMVNullPuromycin3FLAG(C-terminal)Academic: 1301
Industry: 2602
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P-ALV-B09OverexpressionpGMLV-CMV-MCS-3flag-T2A-ZsGreenCMVZsGreenNull3FLAG(C-terminal)Academic: 1198
Industry: 2396
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P-ALV-B10OverexpressionpGMLV-CMV-MCS-T2A-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B11OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycin3FLAG(C-terminal)Academic: 1198
Industry: 2396
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P-ALV-B12OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B13OverexpressionpGMLV-CMV-MCS-T2A-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B14OverexpressionpGMLV-CMV-MCS-dsRed-PGK-PuromycinCMVdsRedPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B15OverexpressionpGMLV-CMV-MCS-IRES-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B16OverexpressionpGMLV-CMV-MCS-IRES-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B17OverexpressionpGMLV-CMV-MCS-PGK-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B18OverexpressionpGMLV-CMV-MCS-PGK-Puromycin-IRES-ZsGreenCMVZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B19shRNA for RNAipGMLV-U6-MCS-PGK-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B20shRNA for RNAipGMLV-U6-MCS-CMV-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B21shRNA for RNAipGMLV-U6-MCS-CMV-ZsGreen-PGK-PuromycinU6ZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B22OverexpressionpGMLV-EM7-MCS-Ubc-rtTA3-PuromycinEM7NullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B23OverexpressionpGMLV-PGK-Puromycin-Tnl-EGFPPGKEGFPPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B24OverexpressionpGMLV-CMV-MCS-PGK-RFPCMVRFPNullNULLAcademic: 1301
Industry: 2602
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P-ALV-B25OverexpressionpGMLV-CMV-MCS-PGK-Z418CMVNullZ418NULLAcademic: 1301
Industry: 2602
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P-ALV-B26shRNA for RNAipGMLV-U6-MCS-PGK-BSDU6NullBlasticidinNULLAcademic: 1301
Industry: 2602
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P-ALV-B27OverexpressionpGMLV-TetOn-SV40-Puromycin-TRE3GS-MCSTRE3GSNullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B28shRNA for RNAipGMLV-U6-MCS-hPGK-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B29OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-LucCMVRFP/LuciferaseNullNULLAcademic: 2506
Industry: 5012
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P-ALV-B30OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-PuromycinCMVRFPPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B31OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-LucCMVZsGreen/LuciferaseNullNULLAcademic: 2386
Industry: 4772
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P-ALV-B32shRNA for RNAipGMLV-U6-MCS-EF1-Luc-T2A-PuromycinU6LuciferasePuromycinNULLAcademic: 1546
Industry: 3092
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P-ALV-B33shRNA for RNAipGMLV-U6-MCS-EF1-RFP-T2A-LucU6RFP/LuciferaseNullNULLAcademic: 2506
Industry: 5012
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P-ALV-B34shRNA for RNAipGMLV-U6-MCS-EF1-RFP-T2A-PuromycinU6RFPPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B35shRNA for RNAipGMLV-U6-MCS-EF1-ZsGreen-T2A-LucU6ZsGreen/LuciferaseNullNULLAcademic: 2386
Industry: 4772
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P-ALV-B36shRNA for RNAipGMLV-U6-MCS-EF1-ZsGreen-T2A-BSDU6ZsGreenBlasticidinNULLAcademic: 1426
Industry: 2852
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P-ALV-B37OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-BSDCMVZsGreenBlasticidinNULLAcademic: 1426
Industry: 2852
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P-ALV-B38shRNA for RNAipGMLV-U6-MCS-EF1-fLuc-T2A-mCherryU6mCherry/LuciferaseNullNULLAcademic: 2506
Industry: 5012
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P-ALV-B39OverexpressionpGMLV-CMV-MCS-EF1-fLuc-T2A-mcherryCMVmCherry/LuciferaseNullNULLAcademic: 2506
Industry: 5012
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P-ALV-B40shRNA for RNAipGMLV-U6-MCS-EF1-fLuc-T2A-BSDU6LuciferaseBlasticidinNULLAcademic: 2386
Industry: 4772
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P-ALV-B41shRNA for RNAipGMLV-U6-MCS-PGK-Z418U6NullZ418NULLAcademic: 1301
Industry: 2602
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P-ALV-B42OverexpressionpGMLV-CMV-MCS-fLUC-EF1-ZsGreen-T2A-PuromycinCMVZsGreen/LuciferasePuromycinNULLAcademic: 3466
Industry: 6932
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P-ALV-B43OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin(I)CMVZsGreenPuromycin3FLAG(C-terminal)Academic: 1198
Industry: 2396
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P-ALV-B44OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-fLUC(I)CMVZsGreen/LuciferaseNullNULLAcademic: 2386
Industry: 4772
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P-ALV-B45shRNA for RNAipGMLV-U6-MCS-CMV-mCherry-P2A-BSDU6mCherryBlasticidinNULLAcademic: 1666
Industry: 3332
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P-ALV-B46shRNA for RNAipGMLV-U6-MCS-CMV-EGFP-P2A-BSDU6EGFPBlasticidinNULLAcademic: 1426
Industry: 2852
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P-ALV-B47OverexpressionpGMLV-CMV-DIO-ZsGreen-P2A-MCS-DIO-EF1-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B48OverexpressionpGMLV-CMV-MCS-P2A-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B49gRNApGMLV-U6-gRNA-EF1-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B50cas9/gRNApGMLV-U6-gRNA-EF1-CAS9-PuromycinU6NullPuromycin1FLAGAcademic: 1301
Industry: 2602
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P-ALV-B51shRNA for RNAipGMLV-U6-MCS-EF1-mCherry-T2A-PuromycinU6mCherryPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B52OverexpressionpGMLV-CMV-MCS-EF1-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B53OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreenCMVZsGreenNull3FLAG(C-terminal)Academic: 1198
Industry: 2396
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P-ALV-B54OverexpressionpCDH-MSCV-MCS-3flag-EF1-copGFP-T2A-PuromycinMSCVcopGFPPuromycin3FLAG(C-terminal)Academic: 1198
Industry: 2396
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P-ALV-B55gRNApGMLV-U6-gRNA-EF1-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B56OverexpressionpGMLV-CMV-MCS-EF1-fLUC-T2A-BSDCMVLuciferaseBlasticidinNULLAcademic: 2386
Industry: 4772
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P-ALV-B57OverexpressionpGMLV-CMV-MCS-EF1-Cas9-P2A-BSDCMVNullBlasticidinNULLAcademic: 1301
Industry: 2602
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P-ALV-B58OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-HygroCMVRFPhygroNULLAcademic: 1666
Industry: 3332
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P-ALV-B59shRNA for RNAipGMLV-U6-CMV-RFP-P2A-BSD-NU6RFPBlasticidinNULLAcademic: 1666
Industry: 3332
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P-ALV-B60OverexpressionpGMLV-CMV-EF1-ZsGreen-P2A-BSDCMVZsGreenBlasticidinNULLAcademic: 1426
Industry: 2852
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P-ALV-B61OverexpressionpGMLV-CMV-MCS-T2A-mCherryCMVmCherryNullNULLAcademic: 1301
Industry: 2602
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P-ALV-B62shRNA for RNAipGMLV-U6-CMV-EGFP-P2A-BSDU6EGFPBlasticidinNULLAcademic: 1426
Industry: 2852
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P-ALV-B63OverexpressionpGMLV-DIO-ZsGreen-P2A-MCS-DIO-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B64OverexpressionpGMLV-CMV-EF1-ZsGreen-P2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B65OverexpressionpGMLV-CMV-P2A-ZsGreen-P2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B66OverexpressionpGMLV-MCS-EF1-ZsGreen-T2A-PuromycinNullZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B67LC3pGMLV-CMV-EGFP-LC3-T2A-PuromycinCMVEGFPPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B68LC3pGMLV-CMV-mCherry-EGFP-LC3-T2A-PuromycinCMVEGFP/mCherryPuromycinNULLAcademic: 3466
Industry: 6932
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P-ALV-B69cas9/gRNApGMLV-U6-scramble-EF1-cas9-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B70cas9/gRNApGMLV-U6-scramble-CMV-Cas9-T2A-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B71cas9/gRNApGMLV-U6-scramble-EF1-Cas9-P2A-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B72OverexpressionpGMLV-CMV-MCS-EF1-Z418CMVNullZ418NULLAcademic: 1301
Industry: 2602
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P-ALV-B73OverexpressionpGMLV-CMV-MCS-3flag-EF1-mCherry-P2A-BSDCMVmCherryBlasticidin3FLAG(C-terminal)Academic: 1666
Industry: 3332
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P-ALV-B74shRNA for RNAipGMLV-U6-MCS-PGK-Z418(E)U6NullZ418NULLAcademic: 1301
Industry: 2602
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P-ALV-B75shRNA for RNAipGMLV-U6-gRNA-PGK-BSDU6NullBlasticidinNULLAcademic: 1301
Industry: 2602
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P-ALV-B76OverexpressionpGMLV-EF1-T2A-MCS-ZsGreenEF1ZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B77cas9/gRNApGMLV-U6-gRNA-EF1-mCherry-BSDU6mCherryBlasticidinNULLAcademic: 1666
Industry: 3332
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P-ALV-B78OverexpressionpGMLV-CMV-DIO-EF1-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B79OverexpressionpGMLV-CMV-Circ-MCS-EF1-ZsGreenCMVZsGreenNullNULLAcademic: 1918
Industry: 3836
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P-ALV-B80OverexpressionpGMLV-CMV-cre-3flag-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycin3FLAG(C-terminal)Academic: 1198
Industry: 2396
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P-ALV-B81OverexpressionpGMLV-EF1-MCS-CMV-PuromycinEF1NullPuromycinNULLAcademic: 1301
Industry: 2602
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P-ALV-B82OverexpressionpGMLV-EF1-MCS-CMV-ZsGreenEF1ZsGreenNullNULLAcademic: 1198
Industry: 2396
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P-ALV-B83OverexpressionpGMLV-EF1-MCS-CMV-ZsGreen-T2A-PuromycinEF1ZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B84OverexpressionpGMLV-EF1-MCS-P2A-ZsGreen-P2A-PuromycinEF1ZsGreenPuromycinNULLAcademic: 1198
Industry: 2396
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P-ALV-B85OverexpressionpGMLV-CMV-MCS-3flag-EF1-mcherryCMVmCherryNull3FLAG(C-terminal)Academic: 1301
Industry: 2602
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P-ALV-B86OverexpressionpGMLV-CMV-MCS-3flag-EF1-eRFP-T2A-PuromycinCMVeRFPPuromycin3FLAG(C-terminal)Academic: 3456
Industry: 6912
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P-ALV-B87shRNA for RNAipGMLV-U6-MCS-EF1-eRFP-T2A-PuromycinU6eRFPPuromycinNULLAcademic: 3456
Industry: 6912
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P-ALV-B88OverexpressionpGMLV-CMV-eRFP-EGFP-LC3-T2A -PuromycinCMVeRFP/EGFPPuromycinNULLAcademic: 3466
Industry: 6932
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P-ALV-B89OverexpressionpGMLV-CMV-circ-EF1-copGFP-PuromycinCMVcopGFPPuromycinNULLAcademic: 1918
Industry: 3836
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P-ALV-B90OverexpressionpGMLV-CMV-LUC-EF1-eRFP-T2A-PuromycinCMVeRFP/LuciferasePuromycinNULLAcademic: 3466
Industry: 6932
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P-ALV-B91OverexpressionpGMLV-CMV-circ-EF1-ZsGreen-T2A-Puromycin-NCMVZsGreenPuromycinNULLAcademic: 1918
Industry: 3836
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Helper plasmids of lentivirus packaging

PMD2G: an envelope protein VSV-G expressing plasmid

Figure 1: Map and sequence of pMD2G

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Picture loading failed. Download the detail information of pMD2G here and read in Snapgene Software.

Click here to download SnapGene Viewer



pSPAX2: lentivirus packaging plasmids expressing Gag-pol and Tat

Figure 2: Map and sequence of pSPAX2

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Picture loading failed. Download the detail information of pSPAX2 here and read in Snapgene Software.

Click here to download SnapGene Viewer