Lentivirus vector system (lentivirus expression system, lentivirus packaging plasmid system)

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Lentivirus vector system - Introduction

GeneMedi's lentivirus Vector System, also named the lentivirus expression system or lentivirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi), gene editing and stable cellline development. You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System.

The Genemedi lentivirus vector system including 3 lentivirus packaging plasmids: the lentivirus expression plasmids with different promoters and tags, an envelope protein VSV-G expressing plasmid pMD2G and a packaging plasmid pSPAX2 expressing Gag-pol and Tat.

Figure 1: Workflow of lentivirus packaging

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GeneMedi has developed a variety of lentivirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's lentivirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA lentivirus expression vector.



Lentivirus vector system


Product Name Organization Type Price(In USD) Qty (Quantity) Sum(In USD)
Lentivirus vector system
Cat.No.:P-PK23
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52)
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
Academic 2430
Lentivirus vector system
Cat.No.:P-PK23
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52)
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
Industry R&D
(excluding
CRO&CDMO&CXO)
Inquiry
Lentivirus vector system
Cat.No.:P-PK23
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52)
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
CRO&CDMO&
CXO&CMC&
Manufacturing company
Inquiry
Shipping Cost: 169.00
Total:

Lentivirus Expressing Plasmid



Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company

Cat.No Product Name Price (in USD) Order
P-ALV-C01 pMD2G Academic: 780; Industry R&D: Inquiry; Others: Inquiry Picture loading failed.
P-ALV-C02 pSPAX2 Academic: 780; Industry R&D: Inquiry; Others: InquiryPicture loading failed.

Lentivirus expression vectors (lentivirus expression plasmids)



Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company

Cat.No Expression Name of Vectors Promoter Reporter Cre-dependent TAG (unfusion) Price (in USD) Order
P-ALV-B01OverexpressionpCDH-MSCV-MCS-EF1-copGFP-T2A-PuromycinMSCVcopGFPPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B02OverexpressionpGMLV-CMV-Circ-MCS-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1918
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B03OverexpressionpGMLV-CMV-MCS-3flag-EF1-fLUC-T2A-PuromycinCMVLuciferasePuromycin3FLAG(C-terminal)Academic: 1546
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B04OverexpressionpGMLV-CMV-MCS-EF1-fLUC-T2A-PuromycinCMVLuciferasePuromycinNULLAcademic: 1546
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B05OverexpressionpGMLV-CMV-MCS-EF1-mCherry-T2A-PuromycinCMVmCherryPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B06OverexpressionpGMLV-CMV-MCS-3flag-EF1-mCherry-T2A-PuromycinCMVmCherryPuromycin3FLAG(C-terminal)Academic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B07OverexpressionpGMLV-CMV-MCS-EF1-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B08OverexpressionpGMLV-CMV-MCS-3flag-EF1-PuromycinCMVNullPuromycin3FLAG(C-terminal)Academic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B09OverexpressionpGMLV-CMV-MCS-3flag-T2A-ZsGreenCMVZsGreenNull3FLAG(C-terminal)Academic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B10OverexpressionpGMLV-CMV-MCS-T2A-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B11OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycin3FLAG(C-terminal)Academic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B12OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B13OverexpressionpGMLV-CMV-MCS-T2A-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B14OverexpressionpGMLV-CMV-MCS-dsRed-PGK-PuromycinCMVdsRedPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B15OverexpressionpGMLV-CMV-MCS-IRES-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B16OverexpressionpGMLV-CMV-MCS-IRES-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B17OverexpressionpGMLV-CMV-MCS-PGK-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B18OverexpressionpGMLV-CMV-MCS-PGK-Puromycin-IRES-ZsGreenCMVZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B19shRNA for RNAipGMLV-U6-MCS-PGK-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B20shRNA for RNAipGMLV-U6-MCS-CMV-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B21shRNA for RNAipGMLV-U6-MCS-CMV-ZsGreen-PGK-PuromycinU6ZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B22OverexpressionpGMLV-EM7-MCS-Ubc-rtTA3-PuromycinEM7NullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B23OverexpressionpGMLV-PGK-Puromycin-Tnl-EGFPPGKEGFPPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B24OverexpressionpGMLV-CMV-MCS-PGK-RFPCMVRFPNullNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B25OverexpressionpGMLV-CMV-MCS-PGK-Z418CMVNullZ418NULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B26shRNA for RNAipGMLV-U6-MCS-PGK-BSDU6NullBlasticidinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B27OverexpressionpGMLV-TetOn-SV40-Puromycin-TRE3GS-MCSTRE3GSNullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B28shRNA for RNAipGMLV-U6-MCS-hPGK-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B29OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-LucCMVRFP/LuciferaseNullNULLAcademic: 2506
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B30OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-PuromycinCMVRFPPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B31OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-LucCMVZsGreen
/Luciferase
NullNULLAcademic: 2386
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B32shRNA for RNAipGMLV-U6-MCS-EF1-Luc-T2A-PuromycinU6LuciferasePuromycinNULLAcademic: 1546
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B33shRNA for RNAipGMLV-U6-MCS-EF1-RFP-T2A-LucU6RFP
/Luciferase
NullNULLAcademic: 2506
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B34shRNA for RNAipGMLV-U6-MCS-EF1-RFP-T2A-PuromycinU6RFPPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B35shRNA for RNAipGMLV-U6-MCS-EF1-ZsGreen-T2A-LucU6ZsGreen
/Luciferase
NullNULLAcademic: 2386
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B36shRNA for RNAipGMLV-U6-MCS-EF1-ZsGreen-T2A-BSDU6ZsGreenBlasticidinNULLAcademic: 1426
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B37OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-BSDCMVZsGreenBlasticidinNULLAcademic: 1426
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B38shRNA for RNAipGMLV-U6-MCS-EF1-fLuc-T2A-mCherryU6mCherry
/Luciferase
NullNULLAcademic: 2506
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B39OverexpressionpGMLV-CMV-MCS-EF1-fLuc-T2A-mcherryCMVmCherry
/Luciferase
NullNULLAcademic: 2506
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B40shRNA for RNAipGMLV-U6-MCS-EF1-fLuc-T2A-BSDU6LuciferaseBlasticidinNULLAcademic: 2386
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B41shRNA for RNAipGMLV-U6-MCS-PGK-Z418U6NullZ418NULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B42OverexpressionpGMLV-CMV-MCS-fLUC-EF1-ZsGreen-T2A-PuromycinCMVZsGreen
/Luciferase
PuromycinNULLAcademic: 3466
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B43OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin(I)CMVZsGreenPuromycin3FLAG(C-terminal)Academic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B44OverexpressionpGMLV-CMV-MCS-EF1-ZsGreen-T2A-fLUC(I)CMVZsGreen
/Luciferase
NullNULLAcademic: 2386
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B45shRNA for RNAipGMLV-U6-MCS-CMV-mCherry-P2A-BSDU6mCherryBlasticidinNULLAcademic: 1666
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B46shRNA for RNAipGMLV-U6-MCS-CMV-EGFP-P2A-BSDU6EGFPBlasticidinNULLAcademic: 1426
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B47OverexpressionpGMLV-CMV-DIO-ZsGreen-P2A-MCS-DIO-EF1-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B48OverexpressionpGMLV-CMV-MCS-P2A-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B49gRNApGMLV-U6-gRNA-EF1-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B50cas9/gRNApGMLV-U6-gRNA-EF1-CAS9-PuromycinU6NullPuromycin1FLAGAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B51shRNA for RNAipGMLV-U6-MCS-EF1-mCherry-T2A-PuromycinU6mCherryPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B52OverexpressionpGMLV-CMV-MCS-EF1-ZsGreenCMVZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B53OverexpressionpGMLV-CMV-MCS-3flag-EF1-ZsGreenCMVZsGreenNull3FLAG(C-terminal)Academic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B54OverexpressionpCDH-MSCV-MCS-3flag-EF1-copGFP-T2A-PuromycinMSCVcopGFPPuromycin3FLAG(C-terminal)Academic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B55gRNApGMLV-U6-gRNA-EF1-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B56OverexpressionpGMLV-CMV-MCS-EF1-fLUC-T2A-BSDCMVLuciferaseBlasticidinNULLAcademic: 2386
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B57OverexpressionpGMLV-CMV-MCS-EF1-Cas9-P2A-BSDCMVNullBlasticidinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B58OverexpressionpGMLV-CMV-MCS-EF1-RFP-T2A-HygroCMVRFPhygroNULLAcademic: 1666
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B59shRNA for RNAipGMLV-U6-CMV-RFP-P2A-BSD-NU6RFPBlasticidinNULLAcademic: 1666
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B60OverexpressionpGMLV-CMV-EF1-ZsGreen-P2A-BSDCMVZsGreenBlasticidinNULLAcademic: 1426
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B61OverexpressionpGMLV-CMV-MCS-T2A-mCherryCMVmCherryNullNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B62shRNA for RNAipGMLV-U6-CMV-EGFP-P2A-BSDU6EGFPBlasticidinNULLAcademic: 1426
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B63OverexpressionpGMLV-DIO-ZsGreen-P2A-MCS-DIO-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B64OverexpressionpGMLV-CMV-EF1-ZsGreen-P2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B65OverexpressionpGMLV-CMV-P2A-ZsGreen-P2A-PuromycinCMVZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B66OverexpressionpGMLV-MCS-EF1-ZsGreen-T2A-PuromycinNullZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B67LC3pGMLV-CMV-EGFP-LC3-T2A-PuromycinCMVEGFPPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B68LC3pGMLV-CMV-mCherry-EGFP-LC3-T2A-PuromycinCMVEGFP
/mCherry
PuromycinNULLAcademic: 3466
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B69cas9/gRNApGMLV-U6-scramble-EF1-cas9-PuromycinU6NullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B70cas9/gRNApGMLV-U6-scramble-CMV-Cas9-T2A-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B71cas9/gRNApGMLV-U6-scramble-EF1-Cas9-P2A-ZsGreenU6ZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B72OverexpressionpGMLV-CMV-MCS-EF1-Z418CMVNullZ418NULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B73OverexpressionpGMLV-CMV-MCS-3flag-EF1-mCherry-P2A-BSDCMVmCherryBlasticidin3FLAG(C-terminal)Academic: 1666
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B74shRNA for RNAipGMLV-U6-MCS-PGK-Z418(E)U6NullZ418NULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B75shRNA for RNAipGMLV-U6-gRNA-PGK-BSDU6NullBlasticidinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B76OverexpressionpGMLV-EF1-T2A-MCS-ZsGreenEF1ZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B77cas9/gRNApGMLV-U6-gRNA-EF1-mCherry-BSDU6mCherryBlasticidinNULLAcademic: 1666
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B78OverexpressionpGMLV-CMV-DIO-EF1-PuromycinCMVNullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B79OverexpressionpGMLV-CMV-Circ-MCS-EF1-ZsGreenCMVZsGreenNullNULLAcademic: 1918
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B80OverexpressionpGMLV-CMV-cre-3flag-EF1-ZsGreen-T2A-PuromycinCMVZsGreenPuromycin3FLAG(C-terminal)Academic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B81OverexpressionpGMLV-EF1-MCS-CMV-PuromycinEF1NullPuromycinNULLAcademic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B82OverexpressionpGMLV-EF1-MCS-CMV-ZsGreenEF1ZsGreenNullNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B83OverexpressionpGMLV-EF1-MCS-CMV-ZsGreen-T2A-PuromycinEF1ZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B84OverexpressionpGMLV-EF1-MCS-P2A-ZsGreen-P2A-PuromycinEF1ZsGreenPuromycinNULLAcademic: 1198
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B85OverexpressionpGMLV-CMV-MCS-3flag-EF1-mcherryCMVmCherryNull3FLAG(C-terminal)Academic: 1301
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B86OverexpressionpGMLV-CMV-MCS-3flag-EF1-eRFP-T2A-PuromycinCMVeRFPPuromycin3FLAG(C-terminal)Academic: 3456
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B87shRNA for RNAipGMLV-U6-MCS-EF1-eRFP-T2A-PuromycinU6eRFPPuromycinNULLAcademic: 3456
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B88OverexpressionpGMLV-CMV-eRFP-EGFP-LC3-T2A-PuromycinCMVeRFP/EGFPPuromycinNULLAcademic: 3466
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B89OverexpressionpGMLV-CMV-circ-EF1-copGFP-PuromycinCMVcopGFPPuromycinNULLAcademic: 1918
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B90OverexpressionpGMLV-CMV-LUC-EF1-eRFP-T2A-PuromycinCMVeRFP
/Luciferase
PuromycinNULLAcademic: 3466
Industry R&D: Inquiry
Others: Inquiry
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P-ALV-B91OverexpressionpGMLV-CMV-circ-EF1-ZsGreen-T2A-Puromycin-NCMVZsGreenPuromycinNULLAcademic: 1918
Industry R&D: Inquiry
Others: Inquiry
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Helper plasmids of lentivirus packaging

PMD2G: an envelope protein VSV-G expressing plasmid

Figure 1: Map and sequence of pMD2G

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Picture loading failed. Download the detail information of pMD2G here and read in Snapgene Software.

Click here to download SnapGene Viewer



pSPAX2: lentivirus packaging plasmids expressing Gag-pol and Tat

Figure 2: Map and sequence of pSPAX2

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Picture loading failed. Download the detail information of pSPAX2 here and read in Snapgene Software.

Click here to download SnapGene Viewer




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