Picture loading failed.

pdf downloadLentivirus protocol Download pdf downloadLentivirus User Manual Download

Lentivirus vector system - Introduction

GeneMedi's lentivirus Vector System, also named the lentivirus expression system or lentivirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi), gene editing and stable cellline development. You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System.

The Genemedi lentivirus vector system including 3 lentivirus packaging plasmids: the lentivirus expression plasmids with different promoters and tags, an envelope protein VSV-G expressing plasmid pMD2G and a packaging plasmid pSPAX2 expressing Gag-pol and Tat.

Figure 1: Workflow of lentivirus packaging

Picture loading failed.

GeneMedi has developed a variety of lentivirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's lentivirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA lentivirus expression vector.

Lentivirus vector system

Product Name Contents Price(In USD) Qty (Quantity) Sum(In USD)
Lentivirus vector system 1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors: pCDH-MSCV-MCS-EF1-copGFP-T2A-Puromycin (Cat No.: P-ALV-B01)
(Expression vector refers only to B01, if you need other expression vector, please check the table below)

PMD2G Cat No.: P-ALV-C01; size: 5ug 650
pSPAX2 Cat No.: P-ALV-C02; size: 5ug 650
Shipping Cost: 169.00

Lentivirus expression vectors (lentivirus expression plasmids)

P-ALV-B01 Overexpression pCDH-MSCV-MCS-EF1-copGFP-T2A-Puromycin MSCV copGFP Puromycin NULL $998
P-ALV-B02 Overexpression pGMLV-CMV-Circ-MCS-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin NULL $1598
P-ALV-B03 Overexpression pGMLV-CMV-MCS-3flag-EF1-fLUC-T2A-Puromycin CMV Luciferase Puromycin 3FLAG(C-terminal) $1288
P-ALV-B04 Overexpression pGMLV-CMV-MCS-EF1-fLUC-T2A-Puromycin CMV Luciferase Puromycin NULL $1288
P-ALV-B05 Overexpression pGMLV-CMV-MCS-EF1-mCherry-T2A-Puromycin CMV mCherry Puromycin NULL $1084
P-ALV-B06 Overexpression pGMLV-CMV-MCS-3flag-EF1-mCherry-T2A-Puromycin CMV mCherry Puromycin 3FLAG(C-terminal) $1084
P-ALV-B07 Overexpression pGMLV-CMV-MCS-EF1-Puromycin CMV Null Puromycin NULL $1084
P-ALV-B08 Overexpression pGMLV-CMV-MCS-3flag-EF1-Puromycin CMV Null Puromycin 3FLAG(C-terminal) $1084
P-ALV-B09 Overexpression pGMLV-CMV-MCS-3flag-T2A-ZsGreen CMV ZsGreen Null 3FLAG(C-terminal) $998
P-ALV-B10 Overexpression pGMLV-CMV-MCS-T2A-ZsGreen CMV ZsGreen Null NULL $998
P-ALV-B11 Overexpression pGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin 3FLAG(C-terminal) $998
P-ALV-B12 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin NULL $998
P-ALV-B13 Overexpression pGMLV-CMV-MCS-T2A-Puromycin CMV Null Puromycin NULL $1084
P-ALV-B14 Overexpression pGMLV-CMV-MCS-dsRed-PGK-Puromycin CMV dsRed Puromycin NULL $1084
P-ALV-B15 Overexpression pGMLV-CMV-MCS-IRES-Puromycin CMV Null Puromycin NULL $1084
P-ALV-B16 Overexpression pGMLV-CMV-MCS-IRES-ZsGreen CMV ZsGreen Null NULL $998
P-ALV-B17 Overexpression pGMLV-CMV-MCS-PGK-ZsGreen CMV ZsGreen Null NULL $998
P-ALV-B18 Overexpression pGMLV-CMV-MCS-PGK-Puromycin-IRES-ZsGreen CMV ZsGreen Puromycin NULL $998
P-ALV-B19 shRNA for RNAi pGMLV-U6-MCS-PGK-Puromycin U6 Null Puromycin NULL $1084
P-ALV-B20 shRNA for RNAi pGMLV-U6-MCS-CMV-ZsGreen U6 ZsGreen Null NULL $998
P-ALV-B21 shRNA for RNAi pGMLV-U6-MCS-CMV-ZsGreen-PGK-Puromycin U6 ZsGreen Puromycin NULL $998
P-ALV-B22 Overexpression pGMLV-EM7-MCS-Ubc-rtTA3-Puromycin EM7 Null Puromycin NULL $1084
P-ALV-B23 Overexpression pGMLV-PGK-Puromycin-Tnl-EGFP PGK EGFP Puromycin NULL $998
P-ALV-B24 Overexpression pGMLV-CMV-MCS-PGK-RFP CMV RFP Null NULL $1084
P-ALV-B25 Overexpression pGMLV-CMV-MCS-PGK-Z418 CMV Null Z418 NULL $1084
P-ALV-B26 shRNA for RNAi pGMLV-U6-MCS-PGK-BSD U6 Null Blasticidin NULL $1084
P-ALV-B27 Overexpression pGMLV-TetOn-SV40-Puromycin-TRE3GS-MCS TRE3GS Null Puromycin NULL $1084
P-ALV-B28 shRNA for RNAi pGMLV-U6-MCS-hPGK-Puromycin U6 Null Puromycin NULL $1084
P-ALV-B29 Overexpression pGMLV-CMV-MCS-EF1-RFP-T2A-Luc CMV RFP/Luciferase Null NULL $2088
P-ALV-B30 Overexpression pGMLV-CMV-MCS-EF1-RFP-T2A-Puromycin CMV RFP Puromycin NULL $1084
P-ALV-B31 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-Luc CMV ZsGreen/Luciferase Null NULL $1988
P-ALV-B32 shRNA for RNAi pGMLV-U6-MCS-EF1-Luc-T2A-Puromycin U6 Luciferase Puromycin NULL $1288
P-ALV-B33 shRNA for RNAi pGMLV-U6-MCS-EF1-RFP-T2A-Luc U6 RFP/Luciferase Null NULL $2088
P-ALV-B34 shRNA for RNAi pGMLV-U6-MCS-EF1-RFP-T2A-Puromycin U6 RFP Puromycin NULL $1084
P-ALV-B35 shRNA for RNAi pGMLV-U6-MCS-EF1-ZsGreen-T2A-Luc U6 ZsGreen/Luciferase Null NULL $1988
P-ALV-B36 shRNA for RNAi pGMLV-U6-MCS-EF1-ZsGreen-T2A-BSD U6 ZsGreen Blasticidin NULL $1188
P-ALV-B37 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-BSD CMV ZsGreen Blasticidin NULL $1188
P-ALV-B38 shRNA for RNAi pGMLV-U6-MCS-EF1-fLuc-T2A-mCherry U6 mCherry/Luciferase Null NULL $2088
P-ALV-B39 Overexpression pGMLV-CMV-MCS-EF1-fLuc-T2A-mcherry CMV mCherry/Luciferase Null NULL $2088
P-ALV-B40 shRNA for RNAi pGMLV-U6-MCS-EF1-fLuc-T2A-BSD U6 Luciferase Blasticidin NULL $1988
P-ALV-B41 shRNA for RNAi pGMLV-U6-MCS-PGK-Z418 U6 Null Z418 NULL $1084
P-ALV-B42 Overexpression pGMLV-CMV-MCS-fLUC-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen/Luciferase Puromycin NULL $2888
P-ALV-B43 Overexpression pGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin(I) CMV ZsGreen Puromycin 3FLAG(C-terminal) $998
P-ALV-B44 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-fLUC(I) CMV ZsGreen/Luciferase Null NULL $1988
P-ALV-B45 shRNA for RNAi pGMLV-U6-MCS-CMV-mCherry-P2A-BSD U6 mCherry Blasticidin NULL $1388
P-ALV-B46 shRNA for RNAi pGMLV-U6-MCS-CMV-EGFP-P2A-BSD U6 EGFP Blasticidin NULL $1188
P-ALV-B47 Overexpression pGMLV-CMV-DIO-ZsGreen-P2A-MCS-DIO-EF1-Puromycin CMV ZsGreen Puromycin NULL $998
P-ALV-B48 Overexpression pGMLV-CMV-MCS-P2A-ZsGreen CMV ZsGreen Null NULL $998
P-ALV-B49 gRNA pGMLV-U6-gRNA-EF1-Puromycin U6 Null Puromycin NULL $1084
P-ALV-B50 cas9/gRNA pGMLV-U6-gRNA-EF1-CAS9-Puromycin U6 Null Puromycin 1FLAG $1084
P-ALV-B51 shRNA for RNAi pGMLV-U6-MCS-EF1-mCherry-T2A-Puromycin U6 mCherry Puromycin NULL $1084
P-ALV-B52 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen CMV ZsGreen Null NULL $998
P-ALV-B53 Overexpression pGMLV-CMV-MCS-3flag-EF1-ZsGreen CMV ZsGreen Null 3FLAG(C-terminal) $998
P-ALV-B54 Overexpression pCDH-MSCV-MCS-3flag-EF1-copGFP-T2A-Puromycin MSCV copGFP Puromycin 3FLAG(C-terminal) $998
P-ALV-B55 gRNA pGMLV-U6-gRNA-EF1-ZsGreen U6 ZsGreen Null NULL $998
P-ALV-B56 Overexpression pGMLV-CMV-MCS-EF1-fLUC-T2A-BSD CMV Luciferase Blasticidin NULL $1988
P-ALV-B57 Overexpression pGMLV-CMV-MCS-EF1-Cas9-P2A-BSD CMV Null Blasticidin NULL $1084
P-ALV-B58 Overexpression pGMLV-CMV-MCS-EF1-RFP-T2A-Hygro CMV RFP hygro NULL $1388
P-ALV-B59 shRNA for RNAi pGMLV-U6-CMV-RFP-P2A-BSD-N U6 RFP Blasticidin NULL $1388
P-ALV-B60 Overexpression pGMLV-CMV-EF1-ZsGreen-P2A-BSD CMV ZsGreen Blasticidin NULL $1188
P-ALV-B61 Overexpression pGMLV-CMV-MCS-T2A-mCherry CMV mCherry Null NULL $1084
P-ALV-B62 shRNA for RNAi pGMLV-U6-CMV-EGFP-P2A-BSD U6 EGFP Blasticidin NULL $1188
P-ALV-B63 Overexpression pGMLV-DIO-ZsGreen-P2A-MCS-DIO-Puromycin CMV ZsGreen Puromycin NULL $998
P-ALV-B64 Overexpression pGMLV-CMV-EF1-ZsGreen-P2A-Puromycin CMV ZsGreen Puromycin NULL $998
P-ALV-B65 Overexpression pGMLV-CMV-P2A-ZsGreen-P2A-Puromycin CMV ZsGreen Puromycin NULL $998
P-ALV-B66 Overexpression pGMLV-MCS-EF1-ZsGreen-T2A-Puromycin Null ZsGreen Puromycin NULL $998
P-ALV-B67 LC3 pGMLV-CMV-EGFP-LC3-T2A-Puromycin CMV EGFP Puromycin NULL $998
P-ALV-B68 LC3 pGMLV-CMV-mCherry-EGFP-LC3-T2A-Puromycin CMV EGFP/mCherry Puromycin NULL $2888
P-ALV-B69 cas9/gRNA pGMLV-U6-scramble-EF1-cas9-Puromycin U6 Null Puromycin NULL $1084
P-ALV-B70 cas9/gRNA pGMLV-U6-scramble-CMV-Cas9-T2A-ZsGreen U6 ZsGreen Null NULL $998
P-ALV-B71 cas9/gRNA pGMLV-U6-scramble-EF1-Cas9-P2A-ZsGreen U6 ZsGreen Null NULL $998
P-ALV-B72 Overexpression pGMLV-CMV-MCS-EF1-Z418 CMV Null Z418 NULL $1084
P-ALV-B73 Overexpression pGMLV-CMV-MCS-3flag-EF1-mCherry-P2A-BSD CMV mCherry Blasticidin 3FLAG(C-terminal) $1388
P-ALV-B74 shRNA for RNAi pGMLV-U6-MCS-PGK-Z418(E) U6 Null Z418 NULL $1084
P-ALV-B75 shRNA for RNAi pGMLV-U6-gRNA-PGK-BSD U6 Null Blasticidin NULL $1084
P-ALV-B76 Overexpression pGMLV-EF1-T2A-MCS-ZsGreen EF1 ZsGreen Null NULL $998
P-ALV-B77 cas9/gRNA pGMLV-U6-gRNA-EF1-mCherry-BSD U6 mCherry Blasticidin NULL $1388
P-ALV-B78 Overexpression pGMLV-CMV-DIO-EF1-Puromycin CMV Null Puromycin NULL $1084
P-ALV-B79 Overexpression pGMLV-CMV-Circ-MCS-EF1-ZsGreen CMV ZsGreen Null NULL $1598
P-ALV-B80 Overexpression pGMLV-CMV-cre-3flag-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin 3FLAG(C-terminal) $998
P-ALV-B81 Overexpression pGMLV-EF1-MCS-CMV-Puromycin EF1 Null Puromycin NULL $1084
P-ALV-B82 Overexpression pGMLV-EF1-MCS-CMV-ZsGreen EF1 ZsGreen Null NULL $998
P-ALV-B83 Overexpression pGMLV-EF1-MCS-CMV-ZsGreen-T2A-Puromycin EF1 ZsGreen Puromycin NULL $998
P-ALV-B84 Overexpression pGMLV-EF1-MCS-P2A-ZsGreen-P2A-Puromycin EF1 ZsGreen Puromycin NULL $998
P-ALV-B85 Overexpression pGMLV-CMV-MCS-3flag-EF1-mcherry CMV mCherry Null 3FLAG(C-terminal) $1084
P-ALV-B86 Overexpression pGMLV-CMV-MCS-3flag-EF1-eRFP-T2A-Puromycin CMV eRFP Puromycin 3FLAG(C-terminal) $2880
P-ALV-B87 shRNA for RNAi pGMLV-U6-MCS-EF1-eRFP-T2A-Puromycin U6 eRFP Puromycin NULL $2880
P-ALV-B88 Overexpression pGMLV-CMV-eRFP-EGFP-LC3-T2A -Puromycin CMV eRFP/EGFP Puromycin NULL $2888
P-ALV-B89 Overexpression pGMLV-CMV-circ-EF1-copGFP-Puromycin CMV copGFP Puromycin NULL $1598
P-ALV-B90 Overexpression pGMLV-CMV-LUC-EF1-eRFP-T2A-Puromycin CMV eRFP/Luciferase Puromycin NULL $2888
P-ALV-B91 Overexpression pGMLV-CMV-circ-EF1-ZsGreen-T2A-Puromycin-N CMV ZsGreen Puromycin NULL $1598

Helper plasmids of lentivirus packaging

PMD2G: an envelope protein VSV-G expressing plasmid

Figure 1: Map and sequence of pMD2G

Picture loading failed.

Picture loading failed. Download the detail information of pMD2G here and read in Snapgene Software.

Click here to download SnapGene Viewer

pSPAX2: lentivirus packaging plasmids expressing Gag-pol and Tat

Figure 2: Map and sequence of pSPAX2

Picture loading failed.

Picture loading failed. Download the detail information of pSPAX2 here and read in Snapgene Software.

Click here to download SnapGene Viewer