Picture loading failed.

pdf downloadAdenovirus protocol Download pdf downloadAdenovirus User Manual Download

Adenovirus vector system - Introduction

GeneMedi's adenovirus Vector System, also named the adenovirus expression system or adenovirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi) and gene editing. You can easily recombine produce a recombinant adenovirus (r-AdV) particle in HEK293 or HEK293A cell line in high titer using GeneMedi's adenovirus Vector System.

The Genemedi adenovirus vector system including 2 types of adenovirus packaging plasmids: the adenovirus expression plasmids (adenovirus shuttle vectors) with different promoters and tags, and an adenovirus genome backbone plasmid with E1 region deletion.

Figure 1: Workflow of adenovirus packaging

Picture loading failed.

GeneMedi has developed a variety of adenovirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's adenovirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA adenovirus expression vector.

Adenovirus vector system

Adenovirus expression vectors (adenovirus expression plasmids)

Cat.No Expression Name of Vectors Promoter Reporter Selection TAG (unfusion) Price
P-AADV-B01 overexpression pGMAd-EF1a-MCS-CMV-EGFP EF1a EGFP Null NULL $966 
P-AADV-B02 overexpression pGMAd-EF1a-MCS-3flag-CMV-EGFP EF1a EGFP Null 3FLAG(C-terminal) $966 
P-AADV-B03 overexpression pGMAd-EF1a-MCS-CMV-RFP EF1a RFP Null NULL $966 
P-AADV-B04 overexpression/shRNA for RNAi pGMAd-U6-MCS-CMV-MCS U6/CMV Null Null NULL $966 
P-AADV-B05 overexpression pGMAd-U6-MCS-CMV-MCS-3flag U6/CMV Null Null 3FLAG(C-terminal) $966 
P-AADV-B06 overexpression pGMAd-EF1-MCS-CMV-Puromycin EF1a Null Puromycin NULL $966 
P-AADV-B07 overexpression pGMAd-MCMV-MCS-CMV-EGFP MCMV EGFP Null NULL $966 
P-AADV-B08 overexpression pGMAd-MCMV-MCS-3Flag-CMV-EGFP MCMV EGFP Null 3FLAG(C-terminal) $966 
P-AADV-B09 overexpression pGMAd-MCMV-MCS-CMV-RFP MCMV RFP Null NULL $966 
P-AADV-B10 overexpression pGMAd-MCMV-MCS-3flag-CMV-RFP MCMV RFP Null 3FLAG(C-terminal) $966 
P-AADV-B11 overexpression pGMAd-MCMV-MCS-CMV-Luc MCMV luciferaseferase Null NULL $966 
P-AADV-B12 circRNAoverexpression pGMAd-EF1a-circRNA-CMV-EGFP EF1a EGFP Null NULL $966 
P-AADV-B14 shRNA for RNAi pGMAd-U6-MCS-CMV-RFP U6 RFP Null NULL $966 
P-AADV-B15 shRNA for RNAi pGMAd-U6-MCS-CMV-Luc U6 luciferaseferase Null NULL $966 
P-AADV-B16 shRNA for RNAi pGMAd-U6-MCS-CMV-Puromycin U6 Null Puromycin NULL $966 
P-AADV-B17 Cas9/gRNAfor Konckout pGMAd-U6-gRNA-CMV-Cas9-T2A-ZsGreen U6 ZsGreen Null NULL $966 
P-AADV-B18 shRNA for RNAi pGMAd-U6-MCS-CMV-EGFP-△loxp U6 EGFP Null NULL $966 
P-AADV-B19 overexpression pGMAd-EF1-MCS-3flag-CMV-EGFP-△loxp EF1a EGFP Null 3FLAG(C-terminal) $966 
P-AADV-B20 overexpression pGMAd-EF1-MCS-CMV-Puromycin-△loxp EF1a Null Puromycin NULL $966 
P-AADV-B21 circRNAoverexpression pGMAd-EF1-CircRNA-CMV-EGFP-△loxp EF1a EGFP Null NULL $966 
P-AADV-B22 overexpression pGMAd-EF1-MCS-CMV-EGFP-△loxp EF1a EGFP Null NULL $966 
P-AADV-B23 overexpression pGMAd-EF1-MCS-3flag-CMV-mCherry-△loxp EF1a mCherry Null 3FLAG(C-terminal) $966 
P-AADV-B24 overexpression pGMAd-EF1-MCS-3flag-CMV-Luc-△loxp EF1a Luc Null 3FLAG(C-terminal) $966 
P-AADV-B25 overexpression PGMAd-EF1-MCS-3flag-CMV-EYFP EF1a EYFP Null 3FLAG(C-terminal) $966 
P-AADV-B26 shRNA for RNAi pGMAd-U6-MCS-CMV-mCherry U6 mCherry Null NULL $966 
P-AADV-B27 shRNA for RNAi pGMAd-U6-gRNA-CMV-Cas9 U6 Null Null NULL $966 
P-AADV-B28 gRNAfor Konckout pGMAd-U6-gRNA-CMV-mCherry U6 mCherry Null NULL $966 
P-AADV-B29 gRNAfor Konckout pGMAd-U6-gRNA-CMV-EGFP U6 EGFP Null NULL $966 
P-AADV-B30 overexpression pGMAd-EF1-CircRNA EF1a Null Null NULL $966 
P-AADV-B31 shRNA for RNAi pGMAD-U6-MCS-CMV-LUC U6 luciferaseferase Null NULL $966 
P-AADV-B32 overexpression pGMAd-GFAP-T2A-EGFP GFAP EGFP Null NULL $966 
P-AADV-B33 gRNAfor Konckout pGMAd-U6-gRNA-CMV-mCherry(突变) U6 mCherry Null NULL $966 
P-AADV-B34 gRNAfor Konckout pGMAd-U6-gRNA-CMV-Cas9-T2A-ZsGreen(△NLS) U6 ZsGreen Null NULL $966 
P-AADV-B35 gRNAfor Konckout pGMAd-U6-gRNA-CMV-Cas9(△NLS) U6 Null Null NULL $966 
P-AADV-B36 overexpression pGMAD-EF1-MCS-3flag-CMV-eRFP-△loxp EF1a eRFP Null 3FLAG(C-terminal) $966 
P-AADV-B37 shRNA for RNAi pGMAd-U6-MCS-CMV-eRFP U6 eRFP Null NULL $966 

Adenovirus genome backbone plasmid

Product Name Contents Price(In USD) Qty (Quantity) Sum(In USD)
Adenovirus genome backbone plasmid Cat No.: P-AADV5-GB-1;
Size: 5ug
Shipping Cost: 169.00