GeneMedi's SOLIDEX™-ISOEx Immunocytes Cell Isolation Immunomagnetic Beads

• Introduction
• Product list
• Talk with our expert for quote
• Validation data

About GeneMedi SOLIDEX™-ISOEx

SOLIDEX™-ISOEx is GeneMedi's leading matrix of non-destructive cell separation nano-magnetic beads, based on the company's in-house GM-ExBeads™ microsphere modification development platform and high-affinity ligand evolution platform, GM-LIBRA™. The GM-ExBeads™ microsphere modification development platform utilizes 50 nm superparamagnetic iron oxide particles, delivering rapid and stable magnetic responsiveness to ensure controllable and highly reproducible separation processes. Furthermore, it incorporates GM-LIBRA™ engineered recombinant human Fc antibodies to minimize non-specific binding to cells associated with non-human antibodies.

GeneMedi's SOLIDEX™-ISOEx magnetic nanobeads technology encompasses both column-based (ISOEx-Enhanced) and column-free (ISOEx-Easy) variants, delivering versatile solutions tailored to diverse research and clinical needs.

The ISOEx-Enhanced column-based technology from GeneMedi boasts a refined design that leverages amplified magnetic fields within the column, ensuring unparalleled quality for exceptional purity, superior cell viability, and outstanding recovery rates. These magnetic nanobeads offer seamless compatibility with equipment from leading global manufacturers, enabling effortless one-step integrations and smooth transitions to maintain reliable, uninterrupted workflows.

In parallel, GeneMedi's pioneering ISOEx-Easy column-free magnetic nanobeads fuse the high purity, recovery efficiency, and viability-safeguarding advantages of nanoscale innovation with the intuitive convenience of column-free systems—marking a transformative leap in immune cell isolation and purification that drives the industry toward heightened efficiency and user-centric excellence.

Introduction and Applications of SOLIDEX™-ISOEx Immunocytes Cell Isolation Immunomagnetic Beads

GeneMedi SOLIDEX™-ISOEx Cell Isolation Nanobeads provide cutting-edge magnetic separation solutions for comprehensive immune cell isolation. The platform covers essential immune cell types including NK cells, B cells, Pan T cells, CD4+ T cells, and CD8+ T cells. Additionally, GeneMedi also offers extensive selection capabilities targeting critical cellular markers such as CD66b+ cells, CD34+ cells, CD14+ cells, TCR alpha-beta (abT)+ cells, CD138+ cells, CD45RA+ cells, CD45+ cells, CD16+ cells, and CD25+ cells, ensuring ultra-high purity isolation while maintaining cellular integrity throughout the separation process.

GeneMedi SOLIDEX™-ISOEx features three distinct isolation methodologies to meet diverse research requirements. The Positive Selection platform enables direct capture of specific cell populations using antibodies against well-defined surface markers, ideal for applications requiring precisely characterized cellular subsets. The Negative Selection system provides superior preservation of native cellular function through magnetic depletion of non-target cells, yielding completely untouched and functionally pristine target populations. For specific depletion needs, the Depletion strategy allows precise removal of defined cell types from complex mixtures. All three SOLIDEX™-ISOEx platforms are engineered to deliver outstanding performance, providing researchers with flexible solutions that balance high purity requirements with optimal cellular function preservation for both research and clinical applications.

Our magnetic bead-based isolation systems support two flexible labeling strategies to accommodate diverse experimental needs:

Direct Magnetic Activated Cell Separation (Direct MACS)	Indirect Magnetic Activated Cell Separation (Indirect MACS)
Principle: Antibodies are directly conjugated to magnetic beads, allowing for immediate cell capture and isolation. Advantages: Ideal for fast, one-step separation of target cells with minimal handling.	Principle: Antibodies are biotinylated, enabling secondary capture via streptavidin (SA)-coupled magnetic beads. Advantages: Provides enhanced flexibility, particularly when multiplex labeling or sequential isolation is required.

Column-Based Magnetic Separation	Column-Free Magnetic Separation
Principle: This method utilizes a column packed with a ferromagnetic matrix placed within a strong external magnetic field. Cells labeled with 50nm magnetic bead-conjugated antibodies are passed through the column. The powerful magnetic field gradient generated by the matrix traps the labeled target cells, while unlabeled cells flow through. After washing, the isolated target cells are eluted by removing the column from the magnet. Advantages: The key advantage is the high purity of the isolated cell population. The column system creates a high-gradient magnetic field that ensures efficient capture of cells, even those weakly labeled or present in low numbers. This makes it ideal for applications requiring the highest possible purity.	Principle: This streamlined method uses specialized magnetic beads that can be separated directly in standard tubes or other vessels. After incubating the cell sample with the bead-conjugated antibodies, the tube is placed on a suitable magnet (e.g., a magnetic stand). The labeled target cells are attracted to the wall of the tube by the magnetic force, while the unlabeled cells remain in the suspension. The supernatant containing undesired cells is simply decanted or pipetted away. Advantages:The primary advantages are speed, simplicity, and scalability. It is a faster process that does not require specialized columns, making it perfect for quick experiments, processing large sample volumes, or simultaneous isolation of multiple samples. This format is highly suitable for routine isolation and high-throughput workflows.

Key Features & Benefits

1. High-purity isolation of immune and stem cell populations (e.g., T cells, B cells, NK cells, monocytes).

2. Optimized for reproducibility in cell therapy, immunology research, and functional studies.

3. Available in two formats (direct magnetic bead conjugation or biotin-SA magnetic bead coupling) for protocol versatility.

Available Antibody-Magnetic Bead Conjugates

GeneMedi provides a comprehensive selection of monoclonal antibody (mAb) conjugates, including:

1. Anti-Human CD3E, CD4, CD8, CD14, CD19, CD45, CD34, CD138

2. Anti-Human NCAM1 (CD56), TCR alpha-beta (abT), CD45RA

These products are designed to streamline cell separation workflows, ensuring high recovery rates and sample integrity for advanced research applications.

GeneMedi's SOLIDEX™-ISOEx Immunocytes Cell Isolation Immunomagnetic Beads - Product list

Our magnetic bead-based cell isolation portfolio now offers six tailored configurations, combining three critical parameters to match diverse experimental needs: Labeling Type (Direct/Indirect) x Bead Type (Non-releasable/Releasable) x Format (Column/Column-free).

GeneMedi's SOLIDEX™-ISOEx anti-Biotin Nanobeads & L Column-Product list

To meet the diverse requirements of scientific researchers in cell separation, GeneMedi has launched universal capture tools based on the biotin-streptavidin system: SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) and SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Free). These products seamlessly integrate with our optimized SOLIDEX™-ISOEx Nanobeads series, allowing users to select existing SOLIDEX™-ISOEx Nanobeads or utilize anti-Biotin Nanobeads in combination with their own biotinylated antibodies to achieve in vitro enrichment or depletion of cells labeled with one or more biotinylated antibodies/ligands. In practical operations, SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) can be used in combination with SOLIDEX™-ISOEx L Column to provide precise control for column-based separation. After removing the column from the magnetic field, the positively selected cell fraction can be eluted, achieving separation with high purity and high recovery rate.

Product list

Cat.No	Product Name	Description	Products Link
GMP-SMT-219-Ab01-nanoIMB	SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based)	SOLIDEX™ anti-Biotin Nanobeads (Column-Based) are used to enrich or deplete cells labeled with one or more biotinylated antibodies for Column-Based method	Details
GMP-SMT-219-Ab01-nanoIMB-CF	SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Free)	SOLIDEX™ anti-Biotin Nanobeads (Column-Free) are used to enrich or deplete cells labeled with one or more biotinylated antibodies for Column-Free method	Details
GMP-ISOEx-Column-1	SOLIDEX™-ISOEx L Column	SOLIDEX™-ISOEx L Column is developed for the separation of cells labeled with superparamagnetic nanobeads, which can realize the rapid and gentle separation of flowing cells and ensure the stability and activity maintenance of cells.	Details

Validation Data

High-Purity Isolation of TCRα/β+ T Cells from Human PBMCs Using SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Isolation Kit (Column-Based).

Catalog No. GM-Tg-hg-MP2610-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to TCRα/β+ T cell isolation using GeneMedi’s Indirect TCRα/β T Cell Isolation Kit and Company M’s TCRα/β T Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and efficiency. SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Isolation Kit (Column-Based) provides superior performance in isolating TCRα/β+ T cells from human PBMCs, delivering high purity and yield for downstream applications.

Figure 3. High-Purity Isolation of TCRα/β+ T Cells from Human PBMCs Using SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Isolation Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.

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High-Purity Isolation of CD14+ Cells from Human PBMCs Using SOLIDEX™-ISOEx Indirect Human CD14+ Cell Depletion Kit (Column-Based).

Catalog No. GM-Tg-hg-T23212-dep-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to CD14+ cell isolation using GeneMedi’s Indirect Human CD14+ Cell Depletion Kit (Column-Based) and Company M’s CD14+ Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and efficiency. SOLIDEX™-ISOEx Indirect Human CD14+ Cell Depletion Kit (Column-Based) provides superior performance in isolating CD14+ T cells from human PBMCs, delivering high purity and yield for downstream applications.

Figure 4. High-Purity Isolation of CD14+ Cells from Human PBMCs Using SOLIDEX™-ISOEx Indirect Human CD14+ Cell Depletion Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.

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Efficient Isolation of NK Cells Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)

Catalog No.GM-Tg-hg-NK-Cell-iso-kit
To determine the purity of isolated NK cells, CD56+ NK cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with CD3-APC and CD56-PE antibodies and subsequently analyzed by flow cytometry. Results demonstrated a remarkable increase in CD56+ NK cell purity from 8.19% pre-isolated to 94.3% post-isolated, confirming highly effective NK cell enrichment.

Figure 5. Efficient Isolation of NK Cells Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The non-NK cells were labeled with biotinylated antibodies. After incubating with anti-biotin magnetic nanobeads, these cells were depleted by cell isolation column. The NK cells were harvested by collecting the flowthrough component. The NK cells in pre-isolated human PBMCs. NK cells with high purity were enriched.

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High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)

Catalog No.GM-Tg-hg-NK-Cell-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications.

Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.

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Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Based)

Catalog No.GM-Tg-hg-T87075-iso-nanoIMB
To assess the purity of isolated T cells, CD3+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with APC-conjugated CD3 antibody and propidium iodide (PI), followed by flow cytometric analysis. The results demonstrated a significant increase in CD3+ T cell purity from 52.6% pre-isolated to 95.0% post-isolated, confirming successful T cell isolation.

Figure 7. Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Based). The T cells were labeled with SOLIDEX™-ISOEx Nanobeads conjugated with anti-CD3 antibody. And then these cells were enriched by using cell isolation column. The T cells in pre-isolated human PBMCs. T cells with high purity and motility were enriched.

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Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Free)

Catalog No.GM-Tg-hg-T87075-iso-nanoIMB-CF
To evaluate the purity of isolated T cells, CD3+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with APC-conjugated CD3 antibody and analyzed by flow cytometry. The CD3+ T cell purity demonstrated a substantial increase from 65.9% pre-separation to 98.6% post-separation, confirming highly effective T cell isolation.

Figure 8. Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Free). The T cells were labeled with SOLIDEX™-ISOEx Nanobeads conjugated with anti-CD3 antibody. And then these cells were enriched by using magnetic separator. The T cells in pre-isolated human PBMCs. T cells with high purity and motility were enriched.

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Efficient Isolation of CD4+ T Cells Using SOLIDEX™-ISOEx Human CD4 Isolation Nanobeads (Column-Based)

Catalog No. GM-Tg-hg-T10191-iso-nanoIMB
To assess the purity of isolated CD4+ T cells, magnetic separation was performed on human peripheral blood mononuclear cells (PBMCs). Both pre- and post-isolation cells were labeled with CD3-APC and CD4-PE antibodies and subjected to flow cytometric analysis. The purity of CD4+ T cells was significantly enhanced, rising from 28.1% before isolation to 97.0% after isolation, indicating highly efficient enrichment of CD4+ T cells.

Figure 9. High-Efficiency Enrichment of CD4+ Cells with SOLIDEX™-ISOEx Human CD4 Isolation Nanobeads (Column-Based). Cells were incubated with anti-CD4 antibody-conjugated magnetic nanobeads and passed through an isolation column for positive selection. Flow cytometry shows CD4+ T cells in the input PBMC population and the resulting highly purified fraction.

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Efficient Isolation of CD8+ T Cells Using SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-Based)

Catalog No. GM-Tg-hg-TA040-iso-nanoIMB
To determine the purity of isolated CD8+ T cell, CD8+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with CD3-APC and CD8-PE antibodies and subsequently analyzed by flow cytometry. Results demonstrated a remarkable increase in CD8+ T cell purity from 16.6% pre-isolated to 94.6% post-isolated, confirming highly effective CD8+ T cell enrichment.

Figure 10. Efficient Isolation of CD8+ Cells Using SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-Based). The T cells were labeled with magnetic nanobeads conjugated with anti-CD8 antibody. And then these cells were enriched by using cell isolation column. The T cells in pre-isolated human PBMCs . T cells with high purity were enriched.

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