SOLIDEX™ CD14+ Cell Isolation Solution
Introduction of SOLIDEX™ CD14+ Cell Isolation Solution
CD14+ cells primarily refer to monocytes, which are key immune cells in peripheral blood and bone marrow, comprising about 5-10% of the white blood cells. Monocytes play an essential role in innate immunity, differentiating into macrophages or dendritic cells, participating in pathogen phagocytosis, antigen presentation, cytokine secretion (such as IL-6 and TNF-α), and inflammation regulation.
The CD14 antigen is part of the LPS receptor complex. Antibody binding to CD14 does not trigger signal transduction, as CD14 lacks a cytoplasmic domain. CD14 is strongly expressed on most monocytes and macrophages, with weaker expression on neutrophils and some myeloid dendritic cells. GeneMedi offers magnetic beads for the positive selection or depletion of CD14+ cells, catering to different research and therapeutic needs.
Isolation method of SOLIDEX™ CD14+ Cell Isolation Solution
- Positive Selection
- Depletion
Research Applications:
Used to generate macrophages ex vivo, or for cytotoxicity studies and migration assays to assess their function in inflammatory models, such as phagocytic activity or chemotactic factor-induced migration mechanisms.
Clinical Applications:
Positive selection of CD14+ monocytes is used for differentiating into dendritic cells (DCs) for maintenance therapy in pleural mesothelioma patients. Additionally, the method is used to prepare clinical-grade DC vaccines, where autologous DCs loaded with CMV pp65 mRNA have been shown to be safe and feasible in healthy volunteers and HSCT recipients, inducing CMV-specific T cell responses, thereby supporting viral infection prevention.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T23212-iso-nanoIMB | Human CD14 Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T23212-iso-nanoIMB-CF | Human CD14 Nano-mag Beads (Column-Free) | Details |
| GM-Tg-hg-T23212-iso-rel-kit | Indirect Releasable Human CD14+ Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T23212-iso-rel-kit-CF | Indirect Releasable Human CD14+ Cell Isolation Kit (Column-Free) | Details |
| GM-Tg-hg-T23212-iso-kit | Indirect Human CD14+ Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T23212-iso-kit-CF | Indirect Human CD14+ Cell Isolation Kit (Column-Free) | Details |
Research Applications:
Used to study the key role of monocytes in regulating immune molecule expression in pathogenic responses.
Clinical Applications:
Depletion of CD14+ cells followed by positive selection of CD4+ T helper cells from PBMCs is used for efficient separation of CD4+ T helper cells with strong expression, supporting T cell function analysis or transplantation optimization. Moreover, CD14/CD25 depletion removes myeloid cells and Tregs, enhancing CAR-T cell efficacy in the treatment of refractory/relapsed non-Hodgkin lymphoma and reducing immune suppression. In cGMP large-scale production, depletion of CD14+ cells ensures successful CAR-T cell transduction and expansion, increasing production purity and activity.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T23212-dep-nanoIMB | Human CD14+ Cell Depletion Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T23212-dep-kit | Indirect Human CD14+ Cell Depletion Kit (Column-Based) | Details |
