SOLIDEX™-ISOEx CD14+ Cell Isolation Solution
Introduction of SOLIDEX™-ISOEx CD14+ Cell Isolation Solution
CD14+ cells primarily refer to monocytes, which are key immune cells in peripheral blood and bone marrow, comprising about 5-10% of the white blood cells. Monocytes play an essential role in innate immunity, differentiating into macrophages or dendritic cells, participating in pathogen phagocytosis, antigen presentation, cytokine secretion (such as IL-6 and TNF-α), and inflammation regulation.
The CD14 antigen is part of the LPS receptor complex. Antibody binding to CD14 does not trigger signal transduction, as CD14 lacks a cytoplasmic domain. CD14 is strongly expressed on most monocytes and macrophages, with weaker expression on neutrophils and some myeloid dendritic cells. GeneMedi offers magnetic beads for the positive selection or depletion of CD14+ cells, catering to different research and therapeutic needs.
Isolation method of SOLIDEX™-ISOEx CD14+ Cell Isolation Solution
- Positive Selection
- Depletion
Research Applications:
Used to generate macrophages ex vivo, or for cytotoxicity studies and migration assays to assess their function in inflammatory models, such as phagocytic activity or chemotactic factor-induced migration mechanisms.
Clinical Applications:
Positive selection of CD14+ monocytes is used for differentiating into dendritic cells (DCs) for maintenance therapy in pleural mesothelioma patients. Additionally, the method is used to prepare clinical-grade DC vaccines, where autologous DCs loaded with CMV pp65 mRNA have been shown to be safe and feasible in healthy volunteers and HSCT recipients, inducing CMV-specific T cell responses, thereby supporting viral infection prevention.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T23212-iso-nanoIMB | SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) | Details |
| GM-Tg-hg-T23212-iso-nanoIMB-CF | SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-free) | Details |
| GM-Tg-hg-T23212-iso-rel-kit | SOLIDEX™-ISOEx Indirect Releasable Human CD14+ Cell Isolation Kit (Column-based) | Details |
| GM-Tg-hg-T23212-iso-rel-kit-CF | SOLIDEX™-ISOEx Indirect Releasable Human CD14+ Cell Isolation Kit (Column-free) | Details |
| GM-Tg-hg-T23212-iso-kit | SOLIDEX™-ISOEx Indirect Human CD14+ Cell Isolation Kit (Column-based) | Details |
| GM-Tg-hg-T23212-iso-kit-CF | SOLIDEX™-ISOEx Indirect Human CD14+ Cell Isolation Kit (Column-free) | Details |
Research Applications:
Used to study the key role of monocytes in regulating immune molecule expression in pathogenic responses.
Clinical Applications:
Depletion of CD14+ cells followed by positive selection of CD4+ T helper cells from PBMCs is used for efficient separation of CD4+ T helper cells with strong expression, supporting T cell function analysis or transplantation optimization. Moreover, CD14/CD25 depletion removes myeloid cells and Tregs, enhancing CAR-T cell efficacy in the treatment of refractory/relapsed non-Hodgkin lymphoma and reducing immune suppression. In cGMP large-scale production, depletion of CD14+ cells ensures successful CAR-T cell transduction and expansion, increasing production purity and activity.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T23212-dep-nanoIMB | SOLIDEX™-ISOEx Human CD14+ Cell Depletion Nanobeads (Column-based) | Details |
| GM-Tg-hg-T23212-dep-kit | SOLIDEX™-ISOEx Indirect Human CD14+ Cell Depletion Kit (Column-based) | Details |
Validation Data
High-purity isolation of classical monocytes from human peripheral blood mononuclear cells (PBMC) using the SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based)
Catalog No. GM-Tg-hg-T23212-iso-nanoIMB
The SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) was used to isolate and purify CD14+ monocytes from cryopreserved PBMCs. The purity of the cell populations before and after isolation was analyzed by flow cytometry. This experiment achieved a significant increase in CD14+ monocyte purity from 15.2% to 93.7% with a recovery rate of 78.66% as verified by flow cytometry.
Figure 13. High-Purity Isolation of CD14+ Cells from Human PBMCs Using SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based). The flow cytometry analysis of the isolated cells from PBMCs.
High-purity isolation of classical monocytes from human peripheral blood mononuclear cells (PBMC) using the SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Catalog No. GM-CD14-Cell-iso-kit
In this experiment, the SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) was used to isolate and purify classical monocytes from cryopreserved PBMCs. First, non-monocytic cells were specifically labeled with a biotin-conjugated antibody cocktail (Antibody Cocktail), followed by incubation with anti-biotin nano-magnetic beads. The purity of the cell populations before and after isolation was analyzed by flow cytometry. The purity of CD14+ classical monocytes in the flow-through fraction increased from 21.7% to 92.1%. The experimental results demonstrate that this kit effectively removes contaminating cells, yielding high-purity, untouched (antibody-unbound) classical monocytes.
Figure 14. High-purity isolation of classical monocytes from human peripheral blood mononuclear cells (PBMC) using the SOLIDEX™-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based). Flow cytometry results show that the purity of CD14+ classical monocytes increased from 21.7% to 92.1%.
