SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) (Cat No.:GM-Tg-hg-T23212-iso-nanoIMB)

Efficiently isolate Human CD14+ Cells (Monocytes) with SOLIDEX™-ISOEx Nanobeads. Utilizing a robust Positive Selection strategy, this kit specifically targets CD14 antigens to maximize recovery from PBMC or Leukopaks. The Column-based format combined with advanced Magnetic Beads allows for rigorous washing, ensuring High Purity and minimal background contamination. The biodegradable nanobeads are gentle on cells, making them ideal for downstream differentiation into Dendritic Cells (Mo-DCs) or Macrophages without artificial activation.

SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) is a Direct CD14+ Cell Isolation Nanobeads (Column-based, Non-releasable)
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Order information


Catalog No. Product Name Size Price(In USD)
GM-Tg-hg-T23212-iso-nanoIMB-0.1mL SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) 0.1 mL 112
GM-Tg-hg-T23212-iso-nanoIMB-0.5mL SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) 0.5 mL 336
GM-Tg-hg-T23212-iso-nanoIMB-1mL SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) 1 mL 591
GM-Tg-hg-T23212-iso-nanoIMB-7.5mL SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) 7.5 mL

Description


Cat No. GM-Tg-hg-T23212-iso-nanoIMB
Product Name SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based)
Description SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) is a Direct CD14+ Cell Isolation Nanobeads (Column-based, Non-releasable)
Application SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) is used for Positive CD14+ Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Labeling Type Direct
Bead Type Non-releasable
Format Column-based
Key feature Fast isolation; high purity; ideal for automated workflows
Cell population CD14+ Cell
Storage Store at 2℃-8℃

Application

  • Differentiation Studies: Generation of Monocyte-derived Dendritic Cells (Mo-DCs) or Macrophages (M1/M2 polarization).
  • Osteoclastogenesis: Differentiation of monocytes into osteoclasts for bone biology research.
  • Innate Immunity Research: Analysis of cytokine production (e.g., IL-6, TNF-alpha) upon stimulation.
  • Virology: Study of viral entry and replication (e.g., HIV, CMV) in monocytes.

Protocol

  1. Preparation: Prepare a single-cell suspension from Human PBMCs (fresh or frozen) or Leukopaks.
  2. Labeling: Add CD14 Microbeads directly to the cell suspension. Incubate to bind CD14+ Monocytes.
  3. Separation: Place a separation column in the magnetic field and apply the cell suspension.
  4. Washing: Magnetically labeled CD14+ cells are retained in the column; unlabeled cells (T cells, B cells, NK cells) are washed away.
  5. Elution: Remove the column from the magnet and elute the purified CD14+ Cells.

Validation Data

High-purity isolation of classical monocytes from human peripheral blood mononuclear cells (PBMC) using the SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based)

Catalog No. GM-Tg-hg-T23212-iso-nanoIMB
The SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based) was used to isolate and purify CD14+ monocytes from cryopreserved PBMCs. The purity of the cell populations before and after isolation was analyzed by flow cytometry. This experiment achieved a significant increase in CD14+ monocyte purity from 15.2% to 93.7% with a recovery rate of 78.66% as verified by flow cytometry.

Figure 13. High-Purity Isolation of CD14+ Cells from Human PBMCs Using SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads (Column-based). The flow cytometry analysis of the isolated cells from PBMCs.

ISOEx Cell Isolation Kit & Nanobeads validation
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Background

Why Choose SOLIDEX™-ISOEx Human CD14 Isolation Nanobeads?

  • High Recovery: Positive selection efficiently captures monocytes, ensuring high yields even from donors with lower cell counts.
  • Superior Purity: The column matrix acts as a filter, allowing thorough washing of non-specific cells, resulting in higher purity compared to simple tube-based magnetic capture.
  • Differentiation Ready: The biodegradable nanobeads do not interfere with downstream differentiation into Dendritic Cells or Macrophages.
  • Minimal Activation: Optimized bead size and chemistry prevent artificial activation of monocytes during the isolation process.

FAQ

What are the sample requirements?
This kit is optimized for fresh or cryopreserved Human PBMCs. It is also highly effective with Leukapheresis products (Leukopaks). When using frozen PBMCs, ensure cells are treated with DNase to prevent clumping, which is common with thawing monocytes.
Do the magnetic beads affect cell activation or differentiation?
No. The SOLIDEX™ nanobeads are biodegradable and nano-sized. They are engineered to minimize receptor cross-linking. Validated data shows they do not interfere with the differentiation of monocytes into DCs or Macrophages, nor do they induce artificial cytokine secretion.
How does the Column-based separation work?
The column contains a matrix of ferromagnetic spheres. When placed in a magnetic field, these spheres amplify the magnetic gradient, securely trapping the bead-labeled CD14+ cells. This allows for vigorous washing of contaminants before the target cells are released.
Are the separation columns reusable?
No. To maintain sterility and absolutely prevent cross-contamination between donor samples, the columns are designed for single-use only.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection generally delivers higher purity. The physical column matrix allows for the washing away of non-specific cells that might get trapped in the "clumps" formed by column-free tube methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX™-ISOEx column format is designed to be compatible with most standard automated magnetic cell separators using column technology.