TrueX® AAV Titration & Quantification Solution
Product Introduction & Advantages of TrueX® AAV Titration & Quantification Solution
GeneMedi's TrueX® AAV Titration & Quantification Solution offers a highly sensitive and robust ELISA-based platform designed for the precise measurement of intact Adeno-Associated Virus (AAV) capsids. Accurate titration is a critical quality attribute in gene and cell therapy development. The TrueX® series provides researchers and biomanufacturers with a reliable, standardized method to quantify AAV viral physical titers, ensuring consistency from early-stage discovery through to large-scale biomanufacturing.
TrueX® - ONE Universal AAV Titration Kit (ELISA)
GeneMedi's TrueX® - ONE Universal AAV Titration Kit represents a breakthrough in AAV vector quantification, offering researchers unparalleled convenience for multi-serotype detection in a single ELISA. This innovative kit is specifically designed to simultaneously detect and quantify major AAV serotypes (AAV2, 5, 6, 8, and 9) without the need for serotype-specific reagents.
Key Advantages:
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· True Universal Detection - Single kit format detects all five serotypes (AAV2/5/6/8/9) with equal efficiency, eliminating the need to maintain multiple serotype-specific kits
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· Streamlined Workflow - Simplified experimental design reduces setup time and minimizes potential errors associated with running multiple assays
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· Cost-Effective Solution - Lower per-sample costs compared to purchasing individual serotype kits, especially for labs working with multiple AAV variants
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· Reduced Hands-On Time - Unified protocol for all serotypes decreases technician workload and increases throughput
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· Ideal for Screening - Perfect for applications requiring rapid serotype identification or when the AAV serotype is unknown
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· Standardized Results - Eliminates inter-kit variability when comparing different serotypes
Detail
TrueX® AAV Titration Solution
For applications demanding the highest specificity, GeneMedi's TrueX® -UNIQ Serotype Specific Titration Kits provide antibody-matched detection systems for each individual AAV serotype (available for AAV2, 5, 6, 8, and 9). These kits offer the most accurate quantification for researchers requiring precise serotype-specific measurements.
Key Advantages:
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· Serotype Precision — Engineered for distinct AAV serotypes (AAV2, AAV5, AAV6, AAV8, and AAV9) to deliver highly accurate intact capsid quantification.
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· Stable & Reliable Components — Reference standards and key reagents are supplied in a rigorously format to guarantee long-term stability, ensuring consistent lot-to-lot performance and reliable data.
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· High Sensitivity & Broad Dynamic Range — Optimized to detect viral particles efficiently, providing a robust analytical window suitable for both highly purified preparations and complex sample matrices.
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· Exceptional Reproducibility — Validated to minimize assay-to-assay variability, ensuring that your crucial analytical workflows remain stable and highly reproducible over time.
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· Streamlined Protocol — Features a user-friendly, standardized ELISA workflow that integrates seamlessly into existing laboratory operations, significantly reducing hands-on time and accelerating overall time-to-result.
Product list for TrueX® AAV Titration & Quantification Solution
| Product Type | Cat No | Product Name | Size | Products Information |
|---|---|---|---|---|
| Universal | GMV-TrueX-ELISA-U-1-96 | TrueX®-ONE Universal AAV Titration (ELISA) | 1 plate (96 wells) | Details |
| Standard | GMV-TrueX-ELISA-AAV2-STD | AAV2 Standard, 1.0E+11 capsids/ml | 120ul | Details |
| Standard | GMV-TrueX-ELISA-AAV5-STD | AAV5 Standard, 1.0E+11 capsids/ml | 120ul | Details |
| Standard | GMV-TrueX-ELISA-AAV6-STD | AAV6 Standard, 1.0E+11 capsids/ml | 120ul | Details |
| Standard | GMV-TrueX-ELISA-AAV8-STD | AAV8 Standard, 1.0E+11 capsids/ml | 120ul | Details |
| Standard | GMV-TrueX-ELISA-AAV9-STD | AAV9 Standard, 1.0E+11 capsids/ml | 120ul | Details |
| AAV2 | GMV-TrueX-ELISA-2-1-96 | TrueX®-AAV2 Serotype Titration Kit (ELISA) | 1 plate (96 wells) | Details |
| AAV5 | GMV-TrueX-ELISA-5-1-96 | TrueX®-AAV5 Serotype Titration Kit (ELISA) | 1 plate (96 wells) | Details |
| AAV6 | GMV-TrueX-ELISA-6-1-96 | TrueX®- AAV6 Serotype Titration Kit (ELISA) | 1 plate (96 wells) | Details |
| AAV8 | GMV-TrueX-ELISA-8-1-96 | TrueX®- AAV8 Serotype Titration Kit (ELISA) | 1 plate (96 wells) | Details |
| AAV9 | GMV-TrueX-ELISA-9-1-96 | TrueX®-AAV9 Serotype Titration Kit (ELISA) | 1 plate (96 wells) | Details |
Validation Data
- TrueX® - AAV Titration Kit (ELISA)
Figure 1. AAV2 capsid titration curve generated using the GenMedi AAV Titration ELISA kit
The table shows the standard concentrations of AAV 2 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9993) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Figure 2. AAV5 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 5 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9995) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy
Figure 3. AAV6 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 6 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9999) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Figure 4. AAV8 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 8 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9998) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Figure 5. AAV9 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 9 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9998) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
