TrueX™ AAV Titration & Quantification Solution
Product Introduction & Advantages of TrueX™ AAV Titration & Quantification Solution
GeneMedi offers two precision solutions for AAV titer quantification: The TrueX™ -ONE Universal AAV Titration Kit (ELISA) 
for simultaneous detection of AAV2/5/6/8/9 in a single ELISA, and TrueX™ -UNIQ Serotype-Specific Titration Kit (ELISA) (AAV2 / AAV5 / AAV6 / AAV8 / AAV9 ) for targeted serotype analysis. Both deliver high sensitivity (2-3×10⁸ vg/mL) in ready-to-use 96-well formats.
The choice between GeneMedi's universal and serotype-specific kits depends on your experimental needs. The TrueX™ -ONE Universal Kit provides exceptional convenience for screening and multi-serotype studies, while the TrueX™ -UNIQ Specific Kits deliver the highest sensitivity for targeted serotype quantification. Both product lines maintain GeneMedi's commitment to accuracy, stability, reliability, and performance excellence in AAV vector characterization.
TrueX™ - ONE Universal AAV Titration Kit (ELISA)
GeneMedi's TrueX™ - ONE Universal AAV Titration Kit represents a breakthrough in AAV vector quantification, offering researchers unparalleled convenience for multi-serotype detection in a single ELISA. This innovative kit is specifically designed to simultaneously detect and quantify major AAV serotypes (AAV2, 5, 6, 8, and 9) without the need for serotype-specific reagents.
Key Advantages:
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· True Universal Detection - Single kit format detects all five serotypes (AAV2/5/6/8/9) with equal efficiency, eliminating the need to maintain multiple serotype-specific kits
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· Streamlined Workflow - Simplified experimental design reduces setup time and minimizes potential errors associated with running multiple assays
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· Cost-Effective Solution - Lower per-sample costs compared to purchasing individual serotype kits, especially for labs working with multiple AAV variants
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· Reduced Hands-On Time - Unified protocol for all serotypes decreases technician workload and increases throughput
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· Ideal for Screening - Perfect for applications requiring rapid serotype identification or when the AAV serotype is unknown
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· Standardized Results - Eliminates inter-kit variability when comparing different serotypes
Detail
TrueX™ - UNIQ AAV Serotype Specific Titration Kit (ELISA)
For applications demanding the highest specificity, GeneMedi's TrueX™ -UNIQ Serotype Specific Titration Kits provide antibody-matched detection systems for each individual AAV serotype (available for AAV2, 5, 6, 8, and 9). These kits offer the most accurate quantification for researchers requiring precise serotype-specific measurements.
Key Advantages:
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· Ultra-Specific Detection - Each kit contains antibodies optimized for a single serotype, minimizing cross-reactivity and false positives
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· Enhanced Sensitivity - Serotype-specific optimization achieves superior detection limits (around 1×107 vg/mL) for each variant
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· Reduced Background Noise - Specialized antibodies decrease non-specific binding, improving signal-to-noise ratios
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· Flexible Purchasing Options - Available as individual serotype kits or as a complete set covering all five serotypes
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· Consistent Performance - Lot-to-lot consistency ensures reproducible results for long-term studies
Product list for TrueX™ AAV Titration & Quantification Solution
| Product Type | Cat No | Product Name | Size | Products Information |
|---|---|---|---|---|
| Universal | GMV-TrueX-ELISA-U-1-96 | TrueX™-ONE Universal AAV Titration | 1 plate/2 plate/5 plate/10 plates (96 wells) | Details |
| AAV2 Specific | GMV-TrueX-ELISA-2-1-96 | TrueX™-UNIQ AAV2 Serotype Specific Titration Kit (ELISA) | 1 plate/2 plate/5 plate/10 plates (96 wells) | Details |
| AAV5 Specific | GMV-TrueX-ELISA-5-1-96 | TrueX™-UNIQ AAV5 Serotype Specific Titration Kit (ELISA) | 1 plate/2 plate/5 plate/10 plates (96 wells) | Details |
| AAV6 Specific | GMV-TrueX-ELISA-6-1-96 | TrueX™-UNIQ AAV6 Serotype Specific Titration Kit (ELISA) | 1 plate/2 plate/5 plate/10 plates (96 wells) | Details |
| AAV8 Specific | GMV-TrueX-ELISA-8-1-96 | TrueX™-UNIQ AAV8 Serotype Specific Titration Kit (ELISA) | 1 plate/2 plate/5 plate/10 plates (96 wells) | Details |
| AAV9 Specific | GMV-TrueX-ELISA-9-1-96 | TrueX™-UNIQ AAV9 Serotype Specific Titration Kit (ELISA) | 1 plate/2 plate/5 plate/10 plates (96 wells) | Details |
Validation Data
- TrueX™ - ONE Universal AAV Titration Kit (ELISA)
- TrueX™ - UNIQ AAV Serotype Specific Titration Kit (ELISA)
Figure 1. AAV2 capsid titration curve generated using the GenMedi AAV Titration ELISA kit
The table shows the standard concentrations of AAV 2 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9998) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Figure 2. AAV5 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 5 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 1.000) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy
Figure 3. AAV6 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 6 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9997) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Figure 4. AAV8 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 8 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9997) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Figure 5. AAV9 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 9 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 1.000) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
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