1. Home

PurProX® AAV Purification Solution



pdf downloadGene Therapy Brochure

Product Introduction & Advantages of PurProX® AAV Purification Solution

GeneMedi's PurProX® is GeneMedi’s proprietary, end-to-end modular system for AAV Downstream Processing (DSP).

Engineered to bridge the "Process Gap" between benchtop discovery and GMP manufacturing, PurProX™ operates on a philosophy of "Unified Chemistry & Linear Scalability." The portfolio encompasses tools ranging from rapid benchtop preparation to kg-scale industrial production. Crucially, all capture products (Spin Columns, Pre-packed Columns, and Resins) are available in both U1 (Broad-Spectrum) and U2 (AAV9 and its variants) formats, delivering high-purity, high-recovery, and scalable vector solutions for every stage of gene therapy development.

PurProX® AAVPure Affinity Resins
Cat.No:
GMV-PurProX-AAVPure-U-1/2


PurProX<sup>®</sup> AAVPure Affinity Resins

GeneMedi PurProX® AAVPure Affinity Resin provide high-performance AAV purification.
U-1: resin broadly targets serotypes like AAV2/5/6/8/DJ/7m8 (with weaker binding for AAV9 compared with other AAV serotypes)
U-2: is specialized for high-affinity AAV9 and its variants binding.

Detail

PurProX® AAVPure Pre-packed Affinity Column
Cat.No:
GMV-PurProX-AAVPure-U-1/2-P


PurProX<sup>®</sup> AAVPure Pre-packed AAV affinity chromatography Column

GeneMedi's PurProX® AAVPure Pre-packed Affinity Column provide a convenient, ready-to-use solution for researchers evaluating our AAV purification resin or performing small-scale AAV vector purification.






Detail

PurProX® AAVEasy rapid-to-purify Spin Column
Cat.No:
GMV-PurProX-AAVEasy-U-1/2-10T

PurProX<sup>®</sup> AAVEasy rapid-to-purify Spin Column

Based on PurProX® AAVPure Affinity Resins, AAVEasy rapid-to-purify Spin Columns provide a rapid, equipment-free solution for small-scale AAV purification, delivering high-purity viral vectors in just 30 minutes.

Detail

PurProX® AAVFull Enrichment Kit
Cat.No:
GMV-PurProX-AAVFull-U-1


PurProX® AAVPure Pre-packed AAV affinity chromatography Column

PurProX® AAVFull empowers you to effortlessly maximize full-capsid ratio with GeneMedi’s seamless Anion Exchange (AEX) chromatography Technology.
· Convenient Syringe Format
-Rapid, hassle-free handling using everyday lab tools.
· Targeted Clearance
-Specifically eliminates empty capsids to yield premium, ultra-pure AAV vectors.
· Ultracentrifuge-Free
-Achieve maximum full-capsid ratios right on your benchtop.

Detail


Product list for PurProX® AAV Purification Solution: Resins, Spin Columns, Pre-packed Columns & Full-Capsid Enrichment

Product Type Cat No Product Name Serotype Size Products Information
AAV Affinity Resin GMV-PurProX-AAVPure-U-1 PurProX® AAVPure Affinity Resin-1 Versatile resin for high-capacity, multi-serotype binding (e.g., AAV2/5/6/8/DJ/7m8) 10 mL, 25 mL, 50 mL, 250 mL, 1 L, 5 L, 10 L Details
AAV Affinity Resin GMV-PurProX-AAVPure-U-2 PurProX® AAVPure Affinity Resin-2 Optimized for AAV9 and variants like AAV-PHP series 10 mL, 25 mL, 50 mL, 250 mL, 1 L, 5 L, 10 L Details
Pre-packed Column GMV-PurProX-AAVPure-U-1-P PurProX® AAVPure Pre-packed Column-1 Versatile resin for high-capacity, multi-serotype binding (e.g., AAV2/5/6/8/DJ/7m8) 1mL,5mL Details
Pre-packed Column GMV-PurProX-AAVPure-U-2-P PurProX® AAVPure Pre-packed Column-2 Optimized for AAV9 and variants like AAV-PHP series 1mL,5mL Details
Spin Column (10 tests) GMV-PurProX-AAVEasy-U-1-10T PurProX® AAVEasy Spin Column-1 Versatile resin for high-capacity, multi-serotype binding (e.g., AAV2/5/6/8/DJ/7m8) 10T Details
Spin Column (10 tests) GMV-PurProX-AAVEasy-U-2-10T PurProX® AAVEasy Spin Column-2 Optimized for AAV9 and variants like AAV-PHP series 10T Details


Validation Data using AAV Purification Platform

  • PurProX® AAVPure Affinity Resin
  • PurProX® AAVPure Pre-packed Column
  • PurProX® AAVEasy Spin Columns
  • PurProX® AAVFull Enrichment Filters

PurProX® AAVPure Affinity Resin

PurProX® AAVPure Affinity Resin-1 for AAV Serotype 2, 5, 6,8, DJ, and 7m8 Purification

The silver staining of purified AAVs by PurProX® AAVPure Affinity Resin-1 (GMV-PurProX-AAVPure-U-1). Company T‘s Resin is used as the control.

AAV8 purified with AAV affinity resin by AKTA
Click to Zoom

PurProX® AAVPure Affinity Resin-2 Designed for AAV9 Purification

The silver staining of purified AAV9 by PurProX® AAVPure Affinity Resin-2 (GMV-PurProX-AAVPure-U-2). Company T‘s Resin is used as the control.

AAV8 purified with AAV affinity resin by AKTA
Click to Zoom

PurProX® AAVPure Pre-packed Column

AAV8 purified with AAV affinity resin by AKTA
Click to Zoom

Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.

Silver staining of AAV8 purification
Click to Zoom

Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.

Silver staining of AAV6 purification
Click to Zoom

Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.

Table.1 Recovery of several serotypes of purified AAV

Serotype Sample Vg Recovery Rate (%)
AAV2 Load 5.72E+12 /
Elution 5.19E+12 90.73
AAV5 Load 3.36E+13 /
Elution 3.13E+13 93.15
AAV6 Load 8.28E+12 /
Elution 7.73E+12 93.36
AAV8 Load 1.33E+13 /
Elution 1.24E+13 93.67

PurProX® AAVEasy Spin Columns

Fig1. AAV5 Purification by PurProX® AAVEasy
Handling Time: 29 mins

Recovery Rate: 95.43%

AAV5 Purification by PURPROXTM AAVEasy
Click to Zoom
Click to Zoom

PurProX® AAVEasy Spin Column-1 for AAV Serotype 2, 5, 6, 8, DJ, and 7m8 Purification

The silver staining of purified AAVs by PurProX® AAVEasy Spin Column-1 (GMV-PurProX-AAVEasy-U-1).

AAV5 Purification by PURPROXTM AAVEasy
Click to Zoom
Click to Zoom

PurProX® AAVEasy Spin Column-2 Designed for AAV9 Purification

The silver staining of purified AAVs by PurProX® AAVEasy Spin Column-2 (GMV-PurProX-AAVEasy-U-2). Company T's Resin (AKTA purification) is used as the control.

AAV5 Purification by PURPROXTM AAVEasy
Click to Zoom
Click to Zoom

PurProX® AAVFull Enrichment Filter

The AAV8 chromatogram demonstrates that this enrichment kit efficiently achieves separation of empty and full capsids, ensuring a final yield of highly pure AAV vectors with a high full-capsid ratio.

AAV8 Capsid Enrichment Chromatogram
Click to Zoom




Gene Therapy
Diagnostics
Biologics
Tarmart
<