PurProX™ AAV Purification Solution
Product Introduction & Advantages of PurProX™ AAV Purification Solution
GeneMedi's PurProX™ AAV Purification Products high-performance solutions for the
isolation and purification of adeno-associated virus (AAV) vectors. Our portfolio includes
PurProX™ AAVPure AAV affinity resins 
and pre-packed AAV affinity chromatography columns , PurProX™ AAVEasy rapid-to-purify spin columns , designed to deliver high purity, excellent
recovery rate, and scalability in different phase of AAV-based gene therapy drug discovery
and development.
GeneMedi PurProX™ offers flexable AAV purification solutions to meet different needs including early discovery, preclinical research, CMC process development and clinical applications.
PurProX™ AAVPure Affinity Resins
Cat.No:
GMV-PurProX-AAVPure-U-1/2
GeneMedi PurProX™ AAVPure Affinity Resins provide high-performance AAV purification. The U-1 resin broadly targets serotypes like AAV2/5/6/8/DJ/7m8 (with weaker binding for AAV9 compared with other AAV serotypes), while the U-2 is specialized for high-affinity AAV9 binding.
PurProX™ AAVPure Pre-packed AAV affinity
chromatography Column
Cat.No:
GMV-PurProX-AAVPure-U-1/2-P0.5ml
GeneMedi's 0.5mL PurProX™ AAVPure
Pre-packed AAV affinity chromatography columns provide a
convenient,
ready-to-use solution for researchers evaluating our AAV
purification resin or performing small-scale AAV vector
purification.
These columns are pre-packed with our
high-performance PurProX™ AAVPure Affinity Resin, offering the
same
exceptional binding capacity and serotype specificity as bulk
resin,
but in an optimized, plug-and-play format.
GeneMedi's PurProX™ AAVPure affinity purification filler redefines the technical prowess of domestic products with its exceptional performance:
-
· Broad-Spectrum Affinity Purification Capability: Compatible with AAV serotypes and various mutants, ensuring flexibility in research and production.
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· High Recovery Efficiency: Achieves a recovery rate exceeding 90%, minimizing waste and boosting yield.
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· Regenerable Use: Supports multiple regeneration cycles, significantly reducing production costs.
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· High Pressure and High Flow Rate: Withstands pressures above 5MPa, perfectly suited for industrial-scale production.
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· Batch-to-Batch Stability: Delivers consistent quality with no ligand shedding, ensuring reliable production outcomes.
With performance rivaling top global brands and a distinct cost-control advantage, GeneMedi PurProX™ AAVPure offers a stable, efficient, and cost-effective solution for the AAV gene therapy industry.
PurProX™ AAVEasy rapid-to-purify Spin Columns
Cat.No:
GMV-PurProX-AAVEasy-U-1/2-10T
Based on PurProX™ AAVPure Affinity Resins,
AAVEasy rapid-to-purify Spin Columns provide a rapid, equipment-free
solution for small-scale AAV purification, delivering high-purity viral
vectors in just 30 minutes.
Designed for simplicity and consistency, these
disposable spin columns eliminate the need for complex chromatography
systems while maintaining excellent recovery
rates (>90%) and purity.
The AAVEasy system features serotype-specific binding for a broad range of AAV serotypes. The U-1 resin targets AAV2, AAV5, AAV6, AAV8, AAV-DJ, and AAV-7m8, while the U-2 resin is specially optimized for high-affinity binding of AAV9. This system requires only standard centrifugation, providing a streamlined and efficient alternative to Iodixanol ultracentrifugation for AAV purification.
For rapid, equipment-free purification, GeneMedi's PurProX™ AAVEasy Purification Spin Columns offer:
· Fast & Simple Protocol -
Purification in <30 minutes, no HPLC/FPLC required
· Single-Use Convenience - Ideal for
small-scale AAV prep
· Consistent Recovery - Reliable
yields for downstream applications
· Ultracentrifuge-free operation - Achieve high-purity AAV prep with just a standard benchtop centrifuge
Product list for PurProX™ AAV Purification Solution
| Product Type | Cat No | Product Name | Serotype | Size | Products Information |
|---|---|---|---|---|---|
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1 | PurProX™ AAVPure Affinity Resin | AAV2, AAV5, AAV6, AAV8, AAV-DJ, and AAV-7m8 | 10 mL, 25 mL, 50 mL, 250 mL, 1 L, 5 L, 10 L | Details |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2 | PurProX™ AAVPure Affinity Resin | AAV9 | 10 mL, 25 mL, 50 mL, 250 mL, 1 L, 5 L, 10 L | Details |
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P0.5ml | PurProX™ AAVPure Pre-packed Column | AAV2, AAV5, AAV6, AAV8, AAV-DJ, and AAV-7m8 | 0.5 mL | Details |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P0.5ml | PurProX™ AAVPure Pre-packed Column | AAV9 | 0.5 mL | Details |
| Spin Column (10 tests) | GMV-PurProX-AAVEasy-U-1-10T | PurProX™ AAVEasy Spin Column | AAV2, AAV5, AAV6, AAV8, AAV-DJ, and AAV-7m8 | 10T | Details |
| Spin Column (10 tests) | GMV-PurProX-AAVEasy-U-2-10T | PurProX™ AAVEasy Spin Column | AAV9 | 10T | Details |
Validation Data
Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.
Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.
Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.
Table.1 Recovery of several serotypes of purified AAV
| Serotype | Sample | Vg | Recovery Rate (%) |
|---|---|---|---|
| AAV2 | Load | 5.72E+12 | / |
| Elution | 5.19E+12 | 90.73 | |
| AAV5 | Load | 3.36E+13 | / |
| Elution | 3.13E+13 | 93.15 | |
| AAV6 | Load | 8.28E+12 | / |
| Elution | 7.73E+12 | 93.36 | |
| AAV8 | Load | 1.33E+13 | / |
| Elution | 1.24E+13 | 93.67 |
