PurProX^® AAV Purification Solution

Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.

Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.

Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.

Fig1. AAV5 Purification by PurProX^® AAVEasy
Handling Time: 29 mins

Recovery Rate: 95.43%

PurProX^® AAVEasy Spin Column-1 for AAV Serotype 2, 5, 6, 8, DJ, and 7m8 Purification

The PAGE of purified AAVs by PurProX® AAVEasy Spin Column-1 (GMV-PurProX-AAVEasy-U-1).

PurProX^® AAVEasy Spin Column-2 Designed for AAV9 Purification

The PAGE of purified AAVs by PurProX® AAVEasy Spin Column-2 (GMV-PurProX-AAVEasy-U-2). Company T's Resin (AKTA purification) is used as the control.

Exceptional AAV5 Purification Performance of PurProX® AAVEasy Spin Column-1 Over 5 Consecutive Regeneration Cycles

Results: Silver staining results demonstrate that the AAV5 sample purified during the 5th use still exhibits distinct and intact VP1/VP2/VP3 capsid protein bands with minimal host cell protein impurities.
Conclusion: The PurProx AAVeasy U1 spin column maintains robust purification performance even after 5 consecutive cycles of purification and regeneration.

High Consistency and Stability Analysis of AAV9 Purification Across 5 Regeneration Cycles Using PurProX® AAVEasy Spin Column-2

Results: The high purity of the eluted products and the sharp capsid protein bands indicate that the U2 spin column can stably and efficiently recover high-quality AAV9 vectors across multiple reuses.
Conclusion: The PurProx AAVeasy U2 spin column exhibits exceptional reusability. After 5 consecutive regeneration cycles, the AAV9 purification efficacy of the U2 column shows no noticeable decline.

Accelerated Stability Test (37°C) of PurProx® AAVeasy Spin Columns: Sustained High Recovery and Capsid Purity

Overview: To evaluate the long-term reliability and shelf-life of PurProX® AAVEasy rapid-to-purify Spin Column, an accelerated stability test was conducted. The spin columns (U1 for AAV5 and U2 for AAV9) were incubated at 37°C for a continuous period of 4 weeks. Purification performance was evaluated at Week 1, Week 2, and Week 4.

Results:
Robust Viral Genome Recovery: Quantitative analysis demonstrates that the binding capacity of the resin remains exceptionally stable under thermal stress. After 1, 2, and 4 weeks at 37°C, the columns maintained a consistently high recovery rate for AAV5 (ranging from ~70% to >80%). There was no significant degradation in column performance over time.

Preserved Capsid Integrity & High Purity: SDS-PAGE analysis of the purified AAV5 and AAV9 vectors showed highly distinct VP1, VP2, and VP3 bands across all time points. The results indicate that the spin columns consistently yield high-purity rAAV particles without inducing aggregation or degradation, even after the columns were subjected to prolonged 37°C exposure.

Conclusion: GeneMedi's PurProX® AAVEasy rapid-to-purify Spin Column exhibit exceptional thermal stability. The accelerated test at 37°C for 4 weeks confirms that the resin matrix is highly robust, ensuring an extended shelf-life and consistent, reproducible AAV purification. Researchers can rely on these columns for maximum recovery and purity, even under fluctuating shipping or room-temperature storage conditions.