TrueX™-ONE Universal AAV Titration Kit (ELISA)

GeneMedi's TrueX™ - ONE Universal AAV Titration Kit represents a breakthrough in AAV vector quantification, offering researchers unparalleled convenience for multi-serotype detection in a single ELISA. This innovative kit is specifically designed to simultaneously detect and quantify major AAV serotypes (AAV2, 5, 6, 8, and 9) without the need for serotype-specific reagents.

TrueX™ AAV Titration & Quantification Solution transfer


TrueX™-ONE Universal AAV Titration
pdfCOA pdfAAV Titration Kit Universal Protocol

Order information


Product Type Catalog No. Product Name Size Price(In USD)
Universal GMV-TrueX-ELISA-U-1-96-1p TrueX™-ONE Universal AAV Titration 1 plate(96 wells)
Universal GMV-TrueX-ELISA-U-1-96-2p TrueX™-ONE Universal AAV Titration 2 plate(96 wells)
Universal GMV-TrueX-ELISA-U-1-96-5p TrueX™-ONE Universal AAV Titration 5 plate(96 wells)
Universal GMV-TrueX-ELISA-U-1-96-10p TrueX™-ONE Universal AAV Titration 10 plate(96 wells)

Description


Catalog number GMV-TrueX-ELISA-U-1-96-1p/2p/5p/10p
Product name TrueX™-ONE Universal AAV Titration Kit (ELISA)
Contents 12 x 8 Determinations
Storage 2-8 °C

Key components

Components Size Format Storage
MTP (Microtiter Plate) 12 x 8-well strips Vacuum packing with desiccant bag 2-8℃
Assay Buffer (20x) 50 mL Tube, dilute before use 2-8℃
Biotin conjugated anti-AAV antibody (370x) 50 μL Tube, dilute before use 2-8℃
HRP-SA (HRP labelled streptavidin, 5000x) 5 μL Tube, dilute before use 2-8℃
TMB 15 mL Tube, ready to use 2-8℃
Stop Solution 15 mL Tube, ready to use 2-8℃

Validation Data


Figure 1. AAV2 capsid titration curve generated using the GenMedi AAV Titration ELISA kit

The table shows the standard concentrations of AAV 2 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9998) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.

AAV2 capsid titration curve generated using the GenMedi AAV Titration ELISA kit
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Figure 2. AAV5 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.

The table shows the standard concentrations of AAV 5 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 1.000) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy

AAV5 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
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Figure 3. AAV6 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.

The table shows the standard concentrations of AAV 6 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9997) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.

AAV2 capsid titration curve generated using the GenMedi AAV Titration ELISA kit
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Figure 4. AAV8 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.

The table shows the standard concentrations of AAV 8 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9997) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.

AAV8 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
Click to Zoom
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Figure 5. AAV9 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.

The table shows the standard concentrations of AAV 9 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 1.000) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.

AAV9 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
Click to Zoom
Click to Zoom

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