For autophagy study, Genemedi supply autophagy flux biosensors encoded by AAV, Adenovirus and lentivirus, which can be used to observe autophagy flux and monitor the intensity of autophagy flux in real-time in vivo or in vitro. Welcome to email [email protected] for consultation.
Taking advantage of RFP-GFP-LC3 and GFP-LC3 labeling system, in which GFP and/or RFP tags are fused at the C-termini of the autophagosome marker LC3, allowing to detect the intensity of autophagy flux in real-time with more accuracy, clarity and intuitiveness. These biosensors provide an enhanced dissection of the maturation of the autophagosome to the autolysosome, which capitalizes on the pH difference between the acidic autolysosome and the neutral autophagosome. The acid-sensitive GFP will be degraded in autolysosome whereas the acid-insensitive RFP will not. Therefore, the change from autophagosome to autolysosome can be visualized by imaging the specific loss of the GFP fluorescence, leaving only red fluorescence.