AAV vector system (AAV expression system, AAV packaging plasmid system)
AAV vector system (AAV expression system, AAV packaging plasmid system)-Introduction
GeneMedi's AAV Vector System, also named AAV expression system or AAV packaging plasmid system, is powerful tool for in-vivo gene delivery, gene editing and gene therapy. You can easily produce recombinant AAV (rAAV) paticle in 293T cell line in high titer using GeneMedi's AAV Vector System. The Genemedi AAV vector system including multiple AAV expression vector plasmids, AAV helper plasmid and the serotypes-specific AAV Rep-Cap plamids.
GeneMedi's AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA AAV expression vector.
The serotypes-specific AAV Rep-Cap plamid (AAV-RC plasmid, or called AAV-RC plasmid) contain the AAV2-Rep gene with different serotypes of AAV's Cap gene(also called AAV capsids gene).
GeneMedi's AAV Rep-Cap plasmids is including AAV2, AAV5, AAV6, AAV8, AAV9, AAV-PHP.B, AAV-PHP.eB, AAV PHP.s, AAV-Retro (Retrograde), AAV-Anc80 (L65), AAV-DJ, AAV-DJ8. GeneMedi also supplies capsid optimized AAV variant including AAV2 variant(Y444F), AAV2 variant (Y272F, Y444F, Y500F, Y730F), AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F), AAV2.7m8, AAV8 variant (Y733F), AAV8 variant(Y733F, Y447F), AAV8 variant(Y733F,Y447F,Y275F) and some other engineering AAV serotypes not mentioned.