AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) vector system (retina-validated AAV expression and packaging plasmid system)

GeneMedi (GM) AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Vector system is including: 1) AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap plasmid (AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-RC plasmid) 2) AAV Helper plasmid 3) AAV expression vectors (overexpression or shRNA).

You can produce AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) particle in 293T cell line in high titer using GeneMedi's AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap plasmid with GM's AAV expression vector (overexpression or shRNA) and AAV helper plasmid.

The tissue tropism of AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) vector has been validated in retina (retinal ganglion cells) by both intravitreal injection and sub-retinal injection., with potential applications in retina gene therapy.

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Product Description

Cat.NO Product Name Organization Type Price(In USD)
P-PK05 AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Vector System
1. AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
Academic 3216
P-PK05 AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Vector System
1. AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
Industry R&D
(excluding
CRO&CDMO&CXO)
P-PK05 AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Vector System
1. AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
CRO&CDMO&
CXO&CMC&
Manufacturing company
P-PK05-Kan AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Vector System(Kan resistance)
1. AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid(Kan resistance), 10mg
2. AAV Helper Plasmid(Kan resistance), 10mg
3. pGMAAV-CMV-MCS-T2A-ZsGreen (Kan resistance) (P-AAVV-B01-Kan), 10mg
All
Currently, GeneMedi only offers amplification services for the Kan-resistant AAV Vector System and do not provide seed stocks.
P-RC05 AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid
Academic 1956
P-RC05 AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid
Industry R&D
(excluding
CRO&CDMO&CXO)
P-RC05 AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid
CRO&CDMO&
CXO&CMC&
Manufacturing company
P-RC05-Kan AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap Plasmid(Kan resistance)
All
Currently, GeneMedi only offers amplification services for the Kan-resistant AAV Rep-Cap Plasmids and do not provide seed stocks.
If you want to obtain higher quantities of plasmids (mg), please visit GM TransExcellent Plasmid DNA Rapid Preparation Service.
Terms of sale:
For Seed (5ug):
The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for any other party.

For plasmid production&lification (≥10mg):
The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for themselves or any other party.

Citation

Title Publication Date
Pituitary hormone α-MSH promotes tumor-induced myelopoiesis and immunosuppression Science 2022/8/4
3’untranslated region of Ckip-1 inhibits cardiac hypertrophy independently of its cognate protein European Heart Journal 2021/5/15
Faecalibacterium Prausnitzii Attenuates CKD via Butyrate-Renal GPR43 Axis CIRCULATION RESEARCH 2022/9/27
Inhibition of METTL3 attenuates renal injury and inflammation by alleviating TAB3 m6A modifications via IGF2BP2-dependent mechanisms Science Translational Medicine 2022/4/13
SIRT3 Acts As a Negative Regulator of Autophagy Dictating Hepatocyte Susceptibility to Lipotoxicity HEPATOLOGY 2017/7/20
TNFR2 Stimulation Promotes Mitochondrial Fusion via Stat3- and NF-kB–Dependent Activation of OPA1 Expression CIRCULATION RESEARCH 2017/8/3
The aberrant upregulation of exon 10-inclusive SREK1 through SRSF10 acts as an oncogenic driver in human hepatocellular carcinoma Nature Communications 2022/3/16
VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes Journal of Hazardous Materials 2023/8/7
NR3C1/Glucocorticoid receptor activation promotes pancreatic β-cell autophagy overload in response to glucolipotoxicity Autophagy 2023/4/11
Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m6A-dependent recognition of Nrf2 mRNA mediated by SND1 JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 2023/5/19


Validation data

transduction of AAV2 variant (Y444F) vector system in mouse retina
Figure. Selected transduction results of AAV2 variant(Y444F) vector system in mouse retina.

Virus and titer: AAV2 variant(Y444F) vector system, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: retina
Gene delivery method: subretinal cavity injection, 3μl
Determine assay: 3 weeks post infection, wholemount, immunofluorescence microscopy


Associated products and services


Products & Service Products & Service Information
AAV Packaging service Detail
Promise-ORF™ viral CDNA library Detail
Plasmid DNA Rapid Preparation Service Detail