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PurProX® AAVPure Affinity Resin — The Engine for Industrial Manufacturing
PurProX® Pre-packed Columns (0.5mL/1mL/5mL) serve as the critical link between R&D and scale-up.
Pre-filled with the exact same AAVPure Affinity Media used in bulk manufacturing, these columns are available in both U1 and U2 versions. Their "Plug-and-Play" design allows researchers to bypass tedious packing procedures, enabling direct connection to FPLC systems (e.g., ÄKTA) for serotype-specific parameter screening and small-scale vector production.
Serotype Specificity Options:
- AAVPure-U1 (Broad-Spectrum): Delivers broad, high-efficiency binding for AAV2, AAV5, AAV6, AAV8, AAV-DJ, and AAV-7m8.
- AAVPure-U2 (AAV9 Specialized): Engineered with a specialized ligand for high-affinity capture of AAV9 and its variants, specifically resolving the low-binding efficiency often seen with conventional resins.
COA
Key Technical Specifications
- Mass Transfer Optimization: Large-pore architecture maintains exceptional Dynamic Binding Capacity (DBC) even under high-velocity industrial flow conditions.
- Mechanical Stability: Rigid polymer matrix withstands backpressures exceeding 1 MPa, perfectly suited for large-scale columns and high-throughput systems.
- High Efficiency: Optimized ligand kinetics ensure consistent recovery rates >90%, maximizing batch yield.
| Serotype | Initial AAV (vg) | Purified AAV (vg) | Recovery Rate (%) |
|---|---|---|---|
| AAV2 | 5.72E+12 | 5.19E+12 | 90.73 |
| AAV5 | 3.36E+13 | 3.13E+13 | 93.15 |
| AAV6 | 8.28E+12 | 7.73E+12 | 93.36 |
| AAV8 | 1.33E+13 | 1.25E+13 | 93.98 |
| AAV9 | 1.45E+13 | 1.37E+13 | 94.48 |
Order Information
| Product Type | Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|---|
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-10ml | PurProX® AAVPure Affinity Resin-1 | 10 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-25ml | PurProX® AAVPure Affinity Resin-1 | 25 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-50ml | PurProX® AAVPure Affinity Resin-1 | 50 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-250ml | PurProX® AAVPure Affinity Resin-1 | 250 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-1L | PurProX® AAVPure Affinity Resin-1 | 1 L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-5L | PurProX® AAVPure Affinity Resin-1 | 5 L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-10ml | PurProX® AAVPure Affinity Resin-2 | 10 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-25ml | PurProX® AAVPure Affinity Resin-2 | 25 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-50ml | PurProX® AAVPure Affinity Resin-2 | 50 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-250ml | PurProX® AAVPure Affinity Resin-2 | 250 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-1L | PurProX® AAVPure Affinity Resin-2 | 1 L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-5L | PurProX® AAVPure Affinity Resin-2 | 5 L |
Related Products
| Product Type | Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|---|
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P1ml | PurProX® AAVPure Pre-packed Column-1 | 1mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P1ml | PurProX® AAVPure Pre-packed Column-2 | 1mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P5ml | PurProX® AAVPure Pre-packed Column-1 | 5mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P5ml | PurProX® AAVPure Pre-packed Column-2 | 5mL |
Description
| Property | Value |
|---|---|
| Catalog number | GMV-PurProX-AAVPure-U-1; GMV-PurProX-AAVPure-U-2 |
| Product name | PurProX® AAVPure Affinity Resin |
| Support matrix | Polymer resin |
| Immobilized ligand | VHH |
| Serotype affinity (U-1) | AAV2, AAV5, AAV6, AAV8, AAV7m8, AAV-DJ |
| Serotype affinity (U-2) | AAV9 |
| Binding capacity | ~3×10¹⁴ vp per mL resin |
| Shipping solvent | 2% Benzyl Alcohol |
| Average particle size | 50 µm |
| Recommended flow rate | ≥3-minute residence time |
| Mechanical resistance | 10 bar (1 MPa) |
| pH range | 2–10 |
| Operating temperature | RT |
| Storage instruction | Do not freeze |
Validation Data
PurProX® AAVPure Affinity Resin-1 for AAV Serotype 2, 5, 6,8, DJ, and 7m8 Purification
Silver staining of purified AAVs by PurProX® AAVPure Affinity Resin-1 (GMV-PurProX-AAVPure-U-1). Company T's Resin is used as the control.
PurProX® AAVPure Affinity Resin-2 Designed for AAV9 Purification
Silver staining of purified AAV9 by PurProX® AAVPure Affinity Resin-2 (GMV-PurProX-AAVPure-U-2). Company T's Resin is used as the control.
Frequently Asked Questions
Is the resin available in a pre-packed column format?
Yes. GeneMedi offers PurProX® AAVPure Pre-packed Columns, pre-filled with the exact same high-performance affinity media used in bulk manufacturing. Available in U1 and U2 versions, in 1 mL and 5 mL sizes.
What is the specificity of the resin?
The resin has a high degree of specificity for intact AAV particles, enabling effective binding of target viral particles while excluding impurities.
Is the resin cost-effective?
Yes. The resin is cost-effective, providing a more competitive price while ensuring high performance, helping to reduce the production costs of gene therapy drugs.
