PurProX™ AAVPure Affinity Resins

GeneMedi PurProX™ AAVPure Affinity Resins are designed for high-capacity binding of multiple AAV serotypes, including AAV2, AAV5, AAV6, AAV8, AAV-DJ, AAV-7m8, (with weaker binding for AAV9 compared with other AAV serotypes).

PurProX™ AAV Purification Solution transfer


PurProX™ AAVPure Affinity Resins
PurProX™ AAVPure Pre-packed AAV affinity chromatography Column
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Order information


Product Type Catalog No. Product Name Size Price(In USD)
AAV Affinity Resin GMV-PurProX-AAVPure-U-1-10ml PurProX™ AAV Affinity Resin 10mL
AAV Affinity Resin GMV-PurProX-AAVPure-U-1-25ml PurProX™ AAV Affinity Resin 25mL
AAV Affinity Resin GMV-PurProX-AAVPure-U-1-50ml PurProX™ AAV Affinity Resin 50mL
AAV Affinity Resin GMV-PurProX-AAVPure-U-1-250ml PurProX™ AAV Affinity Resin 250mL
AAV Affinity Resin GMV-PurProX-AAVPure-U-1-1L PurProX™ AAV Affinity Resin 1L
AAV Affinity Resin GMV-PurProX-AAVPure-U-1-5L PurProX™ AAV Affinity Resin 5L

Related Products

Product Type Catalog No. Product Name Size Price(In USD)
Pre-packed Column GMV-PurProX-AAVPure-U-1-P0.5ml PurProX™ AAVPure Pre-packed Column 0.5mL

Description



Validation Data


AAV8 purified with AAV affinity resin by AKTA
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Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.

Silver staining of AAV8 purification
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Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.

Silver staining of AAV6 purification
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Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.

Table.1 Recovery of several serotypes of purified AAV

Serotype Sample Vg Recovery Rate (%)
AAV2 Load 5.72E+12 /
Elution 5.19E+12 90.73
AAV5 Load 3.36E+13 /
Elution 3.13E+13 93.15
AAV6 Load 8.28E+12 /
Elution 7.73E+12 93.36
AAV8 Load 1.33E+13 /
Elution 1.24E+13 93.67

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