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PurProX® AAVPure Pre-packed Column — The Foundation for Process Development
PurProX® Pre-packed Columns (0.5mL/1mL/5mL) serve as the critical link between R&D and scale-up.
Pre-filled with the exact same AAVPure Affinity Media used in bulk manufacturing, these columns are available in both U1 and U2 versions. Their "Plug-and-Play" design allows researchers to bypass tedious packing procedures, enabling direct connection to FPLC systems (e.g., ÄKTA) for serotype-specific parameter screening and small-scale vector production.
PurProX® AAV Purification Solution
COA
Order Information
| Product Type | Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|---|
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P1ml | PurProX® AAVPure Pre-packed Column-1 | 1 mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P1ml | PurProX® AAVPure Pre-packed Column-2 | 1 mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P5ml | PurProX® AAVPure Pre-packed Column-1 | 5 mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P5ml | PurProX® AAVPure Pre-packed Column-2 | 5 mL |
Related Products
| Product Type | Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|---|
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-10ml | PurProX® AAVPure Affinity Resin-1 | 10 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-25ml | PurProX® AAVPure Affinity Resin-1 | 25 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-50ml | PurProX® AAVPure Affinity Resin-1 | 50 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-250ml | PurProX® AAVPure Affinity Resin-1 | 250 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-1L | PurProX® AAVPure Affinity Resin-1 | 1 L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-5L | PurProX® AAVPure Affinity Resin-1 | 5 L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-10ml | PurProX® AAVPure Affinity Resin-2 | 10 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-25ml | PurProX® AAVPure Affinity Resin-2 | 25 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-50ml | PurProX® AAVPure Affinity Resin-2 | 50 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-250ml | PurProX® AAVPure Affinity Resin-2 | 250 mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-1L | PurProX® AAVPure Affinity Resin-2 | 1 L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-5L | PurProX® AAVPure Affinity Resin-2 | 5 L |
Technical Specifications
| Catalog number | GMV-PurProX-AAVPure-U-1-P; GMV-PurProX-AAVPure-U-2-P |
|---|---|
| Product name | PurProX® AAVPure Pre-packed Column |
| Support matrix | Polymer resin |
| Immobilized ligand | VHH |
| Serotype affinity | GMV-PurProX-AAVPure-U-1-P: Tested-Binding- AAV2, AAV5, AAV6, AAV8, AAV7m8, AAV-DJ; GMV-PurProX-AAVPure-U-2-P: Tested-Binding- AAV9 |
| Binding capacity | ~3E+14 vp per mL resin |
| Shipping solvent | 2% Benzyl Alcohol |
| Average particle size | 50 µm |
| Recommended flow rate | ≥3-minute residence time |
| Mechanical resistance | 10 bar (1 MPa) |
| pH range | 2–10 |
| Operating temperature | RT |
| Storage instruction | Do not freeze |
Validation Data
Fig 1. AAV8 purified with AAV affinity resin by ÄKTA. Chromatogram illustrating the purification of AAV8 on the ÄKTA system. The x-axis represents time; the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.
Fig 2. Silver staining of AAV8 purification. Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60–80 kDa.
Fig 3. Silver staining of AAV6 purification. Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.
Table 1. Recovery of Several Serotypes of Purified AAV
| Serotype | Sample | Vg | Recovery Rate (%) |
|---|---|---|---|
| AAV2 | Load | 5.72E+12 | / |
| Elution | 5.19E+12 | 90.73 | |
| AAV5 | Load | 3.36E+13 | / |
| Elution | 3.13E+13 | 93.15 | |
| AAV6 | Load | 8.28E+12 | / |
| Elution | 7.73E+12 | 93.36 | |
| AAV8 | Load | 1.33E+13 | / |
| Elution | 1.24E+13 | 93.67 |
Frequently Asked Questions
What is the PurProX® AAVPure Pre-packed Column?
The PurProX® AAVPure Pre-packed Column is a ready-to-use affinity chromatography column pre-filled with high-performance AAV purification resin (AAVPure-U-1 or U-2), designed for small-scale AAV vector purification or process parameter screening without manual packing.
What variants are available, and which AAV serotypes do they target?
Two variants are available:
- U-1 — broad-spectrum, effective for AAV2/5/6/8/DJ/7m8
- U-2 — optimized specifically for AAV9 and its variants (e.g., AAV-PHP series).
Is the resin chemistry identical to the bulk AAVPure affinity resin?
Yes. The pre-packed column uses the exact same affinity media as the bulk AAVPure resin, ensuring process homology and enabling linear scale-up from R&D to manufacturing.
Is the column reusable?
Limited reuse is possible with proper regeneration protocols, but single-use is recommended for consistent results in small-scale applications. Contact technical support for regeneration guidelines.
How does it differ from the AAVEasy Spin Column?
The AAVPure pre-packed column requires an FPLC system for precise flow and buffer control — ideal for process development — whereas AAVEasy is a centrifugation-based, equipment-free tool for rapid <30-minute purification. Both share identical resin chemistry but serve different workflow needs.
