PurProX™ AAVPure Pre-packed Column

GeneMedi's 0.5mL PurProX™ AAVPure Pre-packed AAV affinity chromatography columns provide a convenient, ready-to-use solution for researchers evaluating our AAV purification resin or performing small-scale AAV vector purification. These columns are pre-packed with our high-performance PurProX™ AAVPure Affinity Resin, offering the same exceptional binding capacity and serotype specificity as bulk resin, but in an optimized, plug-and-play format.

PurProX™ AAV Purification Solution transfer


PurProX™ AAVPure Pre-packed AAV affinity chromatography Column
pdfCOA pdfPre-packed Column Protocol

Order information


Product Type Catalog No. Product Name Size Price(In USD)
Pre-packed Column GMV-PurProX-AAVPure-U-1-P0.5ml PurProX™ AAVPure Pre-packed Column 0.5mL

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Description


Catalog number GMV-PurProX-AAVPure-U-1-P0.5ml
Product name PurProX™ AAVPure Pre-packed Column
Support matrix Polymer resin
Immobilized ligand VHH
Serotype affinity Tested-Binding: AAV2, AAV5, AAV6, AAV8, AAV7m8, AAV-DJ
Binding capacity ~3E+14 vp per mL resin
Shipping solvent 20% ethanol
Average particle size 50 µm
Recommended flow rate ≥3-minute residence time
Mechanical resistance 100 bar (10 MPa)
pH Range (all ligands) 2-10
Operating temperature RT
Storage instruction Do not freeze

Validation Data


AAV8 purified with AAV affinity resin by AKTA
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Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.

Silver staining of AAV8 purification
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Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.

Silver staining of AAV6 purification
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Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.

Table.1 Recovery of several serotypes of purified AAV

Serotype Sample Vg Recovery Rate (%)
AAV2 Load 5.72E+12 /
Elution 5.19E+12 90.73
AAV5 Load 3.36E+13 /
Elution 3.13E+13 93.15
AAV6 Load 8.28E+12 /
Elution 7.73E+12 93.36
AAV8 Load 1.33E+13 /
Elution 1.24E+13 93.67

Click to get more Data / Case study about the product.