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PurProX® AAVPure Pre-packed Column — The Foundation for Process Development
PurProX® Pre-packed Columns (0.5mL/1mL/5mL) serve as the critical link between R&D and scale-up.
Pre-filled with the exact same AAVPure Affinity Media used in bulk manufacturing, these columns are available in both U1 and U2 versions. Their "Plug-and-Play" design allows researchers to bypass tedious packing procedures, enabling direct connection to FPLC systems (e.g., ÄKTA) for serotype-specific parameter screening and small-scale vector production.
PurProX® AAV Purification Solution
COAOrder PurProX® Pre-packed Columns
| Product Type | Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|---|
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P1ml | PurProX® AAVPure Pre-packed Column-1 | 1mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P1ml | PurProX® AAVPure Pre-packed Column-2 | 1mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P5ml | PurProX® AAVPure Pre-packed Column-1 | 5mL | |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P5ml | PurProX® AAVPure Pre-packed Column-2 | 5mL |
Related Products of PurProX® Pre-packed Columns
| Product Type | Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|---|
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-10ml | PurProX® AAVPure Affinity Resin-1 | 10mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-25ml | PurProX® AAVPure Affinity Resin-1 | 25mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-50ml | PurProX® AAVPure Affinity Resin-1 | 50mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-250ml | PurProX® AAVPure Affinity Resin-1 | 250mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-1L | PurProX® AAVPure Affinity Resin-1 | 1L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1-5L | PurProX® AAVPure Affinity Resin-1 | 5L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-10ml | PurProX® AAVPure Affinity Resin-2 | 10mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-25ml | PurProX® AAVPure Affinity Resin-2 | 25mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-50ml | PurProX® AAVPure Affinity Resin-2 | 50mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-250ml | PurProX® AAVPure Affinity Resin-2 | 250mL | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-1L | PurProX® AAVPure Affinity Resin-2 | 1L | |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2-5L | PurProX® AAVPure Affinity Resin-2 | 5L |
Technical Specifications
| Catalog number | GMV-PurProX-AAVPure-U-1-P;GMV-PurProX-AAVPure-U-2-P |
| Product name | PurProX® AAVPure Pre-packed Column |
| Support matrix | Polymer resin |
| Immobilized ligand | VHH |
| Serotype affinity | GMV-PurProX-AAVPure-U-1-P: Tested-Binding- AAV2, AAV5, AAV6, AAV8, AAV7m8, AAV-DJ; GMV-PurProX-AAVPure-U-2-P: Tested-Binding- AAV9 |
| Binding capacity | ~3E+14 vp per mL resin |
| Shipping solvent | 2% Benzyl Alcohol |
| Average particle size | 50 µm |
| Recommended flow rate | ≥3-minute residence time |
| Mechanical resistance | 10bar (1Mpa) |
| pH Range (all ligands) | 2-10 |
| Operating temperature | RT |
| Storage instruction | Do not freeze |
Validation Data of PurProX® AAVPure Pre-packed Column for different AAV serotypes
Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.
Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.
Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.
Table.1 Recovery of several serotypes of purified AAV
| Serotype | Sample | Vg | Recovery Rate (%) |
|---|---|---|---|
| AAV2 | Load | 5.72E+12 | / |
| Elution | 5.19E+12 | 90.73 | |
| AAV5 | Load | 3.36E+13 | / |
| Elution | 3.13E+13 | 93.15 | |
| AAV6 | Load | 8.28E+12 | / |
| Elution | 7.73E+12 | 93.36 | |
| AAV8 | Load | 1.33E+13 | / |
| Elution | 1.24E+13 | 93.67 |
Frequently Asked Questions (FAQ)
Q1: What is the PurProX® AAVPure Pre-packed Column?
A: The PurProX® AAVPure Pre-packed Column is a ready-to-use affinity chromatography column pre-filled with high-performance AAV purification resin (AAVPure-U-1 or U-2), designed for small-scale AAV vector purification or process parameter screening without manual packing.
Q2: What variants are available, and which AAV serotypes do they target?
A: Two variants are available:
- U-1: Broad-spectrum, effective for AAV2/5/6/8/DJ/7m8;
- U-2: Optimized specifically for AAV9 and its variants (e.g., AAV-PHP series).
Q4: Is the resin chemistry identical to the bulk AAVPure affinity resin?
A: Yes. The pre-packed column uses the exact same affinity media as the bulk AAVPure resin, ensuring process homology and enabling linear scale-up from R&D to manufacturing.
Q8: Is the column reusable?
A: Limited reuse is possible with proper regeneration protocols, but single-use is recommended for consistent results in small-scale applications. Contact technical support for regeneration guidelines.
Q9: How does it differ from the AAVEasy Spin Column?
A: The AAVPure pre-packed column requires an FPLC system for precise flow and buffer control—ideal for process development—whereas AAVEasy is a centrifugation-based, equipment-free tool for rapid <30-minute purification. Both share identical resin chemistry but serve different workflow needs.
