AAV9 Rep-Cap plasmid (serotype 9-specific AAV RC9 plasmids)

GeneMedi's AAV9 Rep-Cap plasmid supplies the AAV2 Rep (replication) proteins and the AAV9 capsid protein.

You can produce AAV9 particle in 293T cell line in high titer using GeneMedi's AAV9 Rep-Cap plasmid with GM's AAV expression vector (overexpression or shRNA) and AAV helper plasmid.

Target products collectionGo to AAV vector system >>



Product Description

Cat.NO Product Name Organization Type Price(In USD) Qty (Quantity) Sum(In USD)
P-RC13 AAV9 Rep-Cap Plasmid
Academic 935
P-RC13 AAV9 Rep-Cap Plasmid
Industry R&D
(excluding
CRO&CDMO&CXO)
P-RC13 AAV9 Rep-Cap Plasmid
CRO&CDMO&
CXO&CMC&
Manufacturing company
P-PK13 AAV9 Vector System
1. AAV9 Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
Academic 2028
P-PK13 AAV9 Vector System
1. AAV9 Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
Industry R&D
(excluding
CRO&CDMO&CXO)
P-PK13 AAV9 Vector System
1. AAV9 Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
CRO&CDMO&
CXO&CMC&
Manufacturing company
If you want to obtain higher quantities of plasmids (mg), please visit GM TransExcellent Plasmid DNA Rapid Preparation Service.
Shipping Cost: 169.00
Total:

Citation

Title Publication Date
LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer Molecular Cancer 2022/7/16
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma Molecular Cancer 2022/5/10
Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice Nature Metabolism 2023/11/20
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice Nature Communications 2023/5/2
Ferritinophagy Mediated by Oxidative Stress-Driven Mitochondrial Damage Is Involved in the Polystyrene Nanoparticles-Induced Ferroptosis of Lung Injury ACS nano 2023/12/12
Endothelial response to type I interferon contributes to vasculopathy and fibrosis and predicts disease progression of systemic sclerosis. Arthritis & Rheumatology 2023/7/24
HuR regulates telomerase activity through TERC methylation NATURE COMMUNICATIONS 2018/6/7
Splicing factor YBX1 regulates bone marrow stromal cell fate during aging The EMBO Journal 2023/3/21
Hexavalent chromium induces γH2AX and RAD51 involved in DNA damage repair in BEAS-2B cells by modulating LNC-DHFR-4: 1 Environment International 2023/3/21
Nanoprodrug ratiometrically integrating autophagy inhibitor and genotoxic agent for treatment of triple-negative breast cancer Biomaterials 2022/3/10


Validation data

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Figure. Selected transduction results of AAV9 in mouse liver.

Virus and titer: AAV9-GFP, AAV9-pTBG-Luciferase (AAV-Luc), 1.4×1012 vg/ml
Animal: mouse, C57, 2 months
Gene delivery method: tail vein, 100μl
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy, in vivo imaging
Conclusion: intravenous injection of AAV9-pTBG-Luciferase (AAV-Luc) only infects liver cells and no non-specific infection

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Figure. Selected transduction results of AAV9-GFP in rat heart (A, B, C) and mouse heart (D).

Virus and titer: AAV9-GFP, 1×1012 vg/ml
Animal: Rat, SD, 2 months (Figure 8A, B, C); mouse, C57, 8 months (Figure 8D)
Infection site: heart
Gene delivery method: myocardial in situ injection, 10μl/site, 5 sites in total (Figure 8A, B, C);intraorbital intravenous injection (Figure 8D)
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

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Figure. Selected transduction results of AAV9-GFP in mouse brain.

Virus and titer: AAV9-GFP, 1×1012 vg/ml
Animal: Mouse, C57, 2 months
Infection site: Brain
Gene delivery method: Brain localization injection, 1μl
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

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Figure. Selected transduction results of AAV in vitro (in 293T cell line)

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×1012 vg/ml
Cells: HEK-293T
MOI: MOI=1×104
Determine assay: 36 hours post infection, immunofluorescence microscopy

transduction results of AAV2 (left) /AAV-DJ (middle) / AAV9 (right) in tumor
Figure. Selected transduction results of AAV2 (left) /AAV-DJ (middle) / AAV9 (right) in tumor.

Virus and titer: AAV2 (left) /AAV-DJ (middle) / AAV9 (right), 1×1012 vg/ml
Animal: Nude mouse, 2 months
Infection site: Subcutaneous transplant of bowel cancer cells
Gene delivery method: Tumor injection, 10μl/site, 4 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

transduction results of AAV9-GFP in mouse hippocampus
Figure. Selected transduction results of AAV9-GFP in mouse hippocampus.

Virus and titer: AAV9-GFAP-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Hippocampus
Gene delivery method: Brain localization injection, 1μl
Determine assay: 3 weeks post infection, wholemount, immunofluorescence microscopy

transduction of AAV9 in mouse skeletal muscle
Figure. Selected transduction results of AAV9 in mouse skeletal muscle.

Virus and titer: AAV9-Luciferase (AAV-Luc), AAV9-GFP, 1×1012 vg/ml
Animal: Mouse, C57, 2 months
Infection site: Skeletal muscles
Gene delivery method: Muscle in situ injection, 10μl/site, 4 sites in total
Determine assay: 4 weeks post infection, in vivo imaging, frozen section, immunofluorescence microscopy

transduction of AAV9-GFP in mouse muscle
Figure. Selected transduction results of AAV9-GFP in mouse muscle.

Virus and titer: AAV-DJ-GFP, AAV9-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Kidney
Gene delivery method: Multiple sites injection in kidney 10μl/site, 6 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

transduction of AAV9-GFP in mouse fat tissue
Figure. Selected transduction results of AAV9-GFP in mouse fat tissue.

Virus and titer: AAV9-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Mammary fat pad
Gene delivery method: Breast injection, 10μl/site, 4 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy


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