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AAV serotype DJ (AAV capsid DJ) system - Introduction

GeneMedi's AAV-DJ Rep-Cap plasmid (serotype -DJ-specific AAV RC plasmid), also named AAV-DJ-RC plasmid, or AAV-RC-DJ plasmid, is part of AAV-DJ packaging system. AAV-DJ Rep-Cap plamid supplies the AAV-DJ Rep (replication) proteins and the AAV-DJ capsid protein.

You can produce AAV-DJ paticle in 293T cell line in high titer using GeneMedi's AAV-DJ Rep-Cap plasmid with GeneMedi's AAV expression vector (overexpression or shRNA) and AAV helper plasmid.

AAV-DJ Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV-DJ capsid protein.

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

Product Name Contents Price(In USD) Qty Sum(In USD)
AAV-DJ Rep-Cap Plasmid Serotype: AAV-DJ
Size: 5ug
490
Shipping Cost: 120.00
Total:

AAV-DJ vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

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Figure. Selected transduction results of AAV9-GFP in mouse muscle.

Virus and titer: AAV-DJ-GFP, AAV9-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Kidney
Gene delivery method: Multiple sites injection in kidney 10μl/site, 6 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy


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Figure. Selected transduction results of AAV in vitro (in 293T cell line)

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×1012 vg/ml
Cells: HEK-293T
MOI: MOI=1×104
Determine assay: 36 hours post infection, immunofluorescence microscopy


Other AAV Rep-Cap Plasmid


AAV1 Rep-Cap Plasmid

AAV2 Rep-Cap Plasmid

AAV2 variant (Y444F) Rep-Cap plasmid

AAV2 variant (Y272F, Y444F, Y500F, Y730F) Rep-Cap plasmid

AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap plasmid

AAV2 variant(AAV2.7m8) Rep-Cap plasmid

AAV5 Rep-Cap Plasmid

AAV-6 Rep-Cap Plasmid

AAV8 Rep-Cap Plasmid

AAV8-1m Rep-Cap plasmid



AAV8-2m Rep-Cap plasmid

AAV8 variant (Y733F, Y447F, Y275) Rep-Cap plasmid

AAV9 Rep-Cap Plasmid

AAV-Rh.10 Rep-Cap Plasmid

AAV-Dj8 Rep-Cap Plasmid

AAV-Retro (Retrograde) Rep-Cap plasmid

AAV9-PHP.B Rep-Cap plasmid

AAV9-PHP.eB Rep-Cap plasmid

AAV9-PHP.S Rep-Cap plasmid