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TrueX® AAV2 Titration ELISA Kit for Capsid Quantification
GeneMedi's TrueX®-UNIQ AAV2 titration ELISA kit provides precise and reliable AAV2 particle quantification through a specialized sandwich ELISA format.
This AAV2 capsid ELISA uses a recombinant antibody that recognizes a conformational epitope on assembled AAV2 capsids, ensuring accurate detection of the AAV2 serotype. Designed for exceptional performance in research settings, the AAV2 ELISA kit delivers reproducible results with high sensitivity for AAV2 capsid quantification. The kit includes all necessary components for efficient AAV2 capsid titration and is optimized for ease of use while maintaining strict quality standards for AAV-based gene and cell therapy development.
TrueX® AAV Titration & Quantification Solution 
COA
TrueX® AAV2 ELISA Kit — Order Information
| Product Type | Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|---|
| AAV2 | GMV-TrueX-ELISA-2-1-96 | TrueX® - AAV2 Serotype Titration Kit (ELISA) | 1 plate(96 wells) |
TrueX® AAV2 Capsid Titration Kit — Specifications
| Catalog number | GMV-TrueX-ELISA-2-1-96 |
| Product name | TrueX® - AAV2 Serotype Titration Kit (ELISA) |
| Contents | 12 x 8 Determinations |
| Storage | 2-8 °C |
TrueX® AAV2 Capsid Titer Kit — Components
| Components | Size | Format | Storage |
|---|---|---|---|
| MTP (Microtiter Plate) | 12 x 8-well strips | Vacuum packing with desiccant bag | 2-8°C |
| Assay Buffer (10x) | 50 mL | Tube, dilute before use | 2-8°C |
| Biotin conjugated anti-AAV antibody (100x) | 150 μL | Tube, dilute before use | 2-8°C |
| HRP-SA (HRP labelled streptavidin, 1000x) | 15 μL | Tube, dilute before use | 2-8°C |
| TMB | 15 mL | Tube, ready to use | 2-8°C |
| Stop Solution | 15 mL | Tube, ready to use | 2-8°C |
TrueX® AAV2 Capsid Quantification — Validation Data
Figure 1. AAV2 capsid titration curve generated using the GenMedi AAV Titration ELISA kit
The table shows the standard concentrations of AAV 2 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9993) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
Click to get more Data / Case study about the product.
TrueX® AAV2 Titration by ELISA — FAQs
What does the TrueX AAV2 capsid ELISA measure?
GMV-TrueX-ELISA-2-1-96 is the AAV2 serotype titration ELISA kit. The assay quantifies AAV2 capsids, with results expressed as capsids/mL.
What sample types can be used for TrueX AAV2 particle quantification?
The TrueX AAV Titration ELISA Kit is designed for quantitative determination of AAV particles in cell culture supernatants and purified virus preparations. The kit is intended for research use only.
How is AAV2 capsid concentration calculated with the TrueX AAV2 ELISA kit?
The AAV2 kit includes lyophilized AAV2 standards. Prepare the standards according to the lot-specific QC sheet and generate a standard curve. Determine the sample concentration by interpolating its optical density against the standard curve, then multiply by the sample dilution factor to obtain the original sample capsid titer in capsids/mL. Samples above the highest standard must be further diluted and reassayed.
How long does the TrueX AAV2 sandwich ELISA take?
The total assay time is approximately 3.5 hours. The assay uses antibody capture of AAV particles, a biotin-conjugated anti-AAV detection antibody, HRP-conjugated streptavidin, and TMB for colorimetric signal generation.
