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AAV serotype DJ (AAV capsid DJ) system - Introduction

GeneMedi's AAV-DJ Vector System (AAV serotype-DJ helper-free packaging plasmids system) is including AAV-DJ Rep-Cap plamid (AAV-DJ-RC plasmid, or called AAV-RC-DJ plasmid), AAV helper plasmid and AAV expression vectors (overexpression or shRNA).

GeneMedi's AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas-DJ-gRNA AAV expression vector.

AAV-DJ Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV-DJ capsid protein.

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

Product Name Contents Price(In USD) Qty Sum(In USD)
AAV-DJ vector system 1. AAV-DJ Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. AAV CMV Expression Vector, 5ug
1060
Shipping Cost: 120.00
Total:

AAV-DJ vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV-DJ vector has been validated in retina, lung, liver and kidney, with potential applications in tissue-specific gene therapy.

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Figure. Selected transduction results of AAV9-GFP in mouse muscle.

Virus and titer: AAV-DJ-GFP, AAV9-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Kidney
Gene delivery method: Multiple sites injection in kidney 10μl/site, 6 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy


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Figure. Selected transduction results of AAV in vitro (in 293T cell line)

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×1012 vg/ml
Cells: HEK-293T
MOI: MOI=1×104
Determine assay: 36 hours post infection, immunofluorescence microscopy


Other AAV vector system


AAV1 vector system

AAV2 vector system

AAV1-mu1 Packaging System

AAV2 variant (Y272F,Y444F,Y500F,Y730F) vector system

AAV2 variant(Y444F,Y730F,Y500F,Y272F,Y704F,Y252F) vector system

AAV2 variant(AAV2.7m8) vector system

AAV5 vector system

AAV6 vector system

AAV8 vector system

AAV8-1m vector system



AAV8-2m vector system

AAV8-3m vector system

AAV9 vector system

AAV-Rh.10 Packaging System

AAV-Dj8 vector system

AAV2-Retro (Retrograde) vector system

AAV9-PHP.B vector system

AAV9-PHP.eB vector system

AAV9-PHP.S vector system