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Capsid mutant-AAV serotype 8 variant (AAV8-(Y733F) system - Introduction

GeneMedi's AAV8-1m Rep-Cap plasmid (retina-validated serotype AAV-RC plasmid), also named AAV8-1m-RC plasmid, is part of AAV8-1m packaging system. AAV8-1m Rep-Cap plamid supplies the AAV8 Rep(replication) proteins and the AAV8-1m capsid protein.

You can produce AAV8-1m paticle in 293T cell line in high titer using GeneMedi's AAV8-1m Rep-Cap plasmid with GeneMedi's AAV expression vector (overexpression or shRNA) and AAV helper plasmid.

The tissue tropism of AAV8 variant(Y733F) vector has been validated in retina with higher transduction efficiency and more rapid onset of expression in photoreceptor cells by both intravitreal injection and sub-retinal injection with potential applications in retina gene therapy.

Product Name Contents Price(In USD) Qty (Quantity) Sum(In USD)
AAV8-1m Rep-Cap plasmid Serotype: AAV8-1m
Size: 5ug
1085
Shipping Cost: 360.00
Total:

AAV8 capsid variant (Y733F) tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV8-1m vector has been validated in retina with higher transduction efficiency and more rapid onset of expression in photoreceptor cells by both intravitreal injection and sub-retinal injection with potential applications in retina gene therapy.

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Figure. Selected transduction results of AAV in vitro (in 293T cell line)

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×1012 vg/ml
Cells: HEK-293T
MOI: MOI=1×104
Determine assay: 36 hours post infection, immunofluorescence microscopy


Other AAV Rep-Cap Plasmid


AAV1 Rep-Cap Plasmid

AAV2 Rep-Cap Plasmid

AAV2 variant (Y444F) Rep-Cap plasmid

AAV2 variant (Y272F, Y444F, Y500F, Y730F) Rep-Cap plasmid

AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) Rep-Cap plasmid

AAV2 variant(AAV2.7m8) Rep-Cap plasmid

AAV5 Rep-Cap Plasmid

AAV6 Rep-Cap Plasmid

AAV8 Rep-Cap Plasmid

AAV8-2m Rep-Cap plasmid



AAV8 variant (Y733F, Y447F, Y275) Rep-Cap plasmid

AAV9 Rep-Cap Plasmid

AAV-Rh.10 Rep-Cap Plasmid

AAV-DJ Rep-Cap Plasmid

AAV-DJ/8 Rep-Cap Plasmid

AAV-Retro (Retrograde) Rep-Cap plasmid

AAV9-PHP.B Rep-Cap plasmid

AAV9-PHP.eB Rep-Cap plasmid

AAV9-PHP.S Rep-Cap plasmid