TrueX®-ONE Universal AAV Titration Kit (ELISA, No Standard)

GeneMedi's TrueX® - ONE Universal AAV Titration Kit (ELISA, No Standard) represents a breakthrough in AAV vector quantification, offering researchers unparalleled convenience for multi-serotype detection in a single ELISA. This innovative kit is specifically designed to simultaneously detect and quantify major AAV serotypes (AAV2, 5, 6, 8, and 9) without the need for serotype-specific reagents.

TrueX® AAV Titration & Quantification Solution

Order Information

Product TypeCatalog No.Product NameSizePrice(In USD)
UniversalGMV-TrueX-ELISA-U-1-96-NS
TrueX®-ONE Universal AAV Titration Kit (ELISA, No Standard)
1 plate(96 wells)
StandardGMV-TrueX-ELISA-AAV2-STDAAV2 Standard, 1.0E+11 capsids/ml120ul$130
StandardGMV-TrueX-ELISA-AAV5-STDAAV5 Standard, 1.0E+11 capsids/ml120ul$130
StandardGMV-TrueX-ELISA-AAV6-STDAAV6 Standard, 1.0E+11 capsids/ml120ul$130
StandardGMV-TrueX-ELISA-AAV8-STDAAV8 Standard, 1.0E+11 capsids/ml120ul$130
StandardGMV-TrueX-ELISA-AAV9-STDAAV9 Standard, 1.0E+11 capsids/ml120ul$130

Description

Catalog numberGMV-TrueX-ELISA-U-1-96-1p-NS
Product name
TrueX®-ONE Universal AAV Titration Kit (ELISA, No Standard)
Contents12 × 8 Determinations
Storage2–8 °C

Key Components

ComponentsSizeFormatStorage
MTP (Microtiter Plate)12 × 8-well stripsVacuum packing with desiccant bag2–8 °C
Assay Buffer (10x)50 mLTube, dilute before use2–8 °C
Biotin conjugated anti-AAV antibody (100x)150 μLTube, dilute before use2–8 °C
HRP-SA (HRP labelled streptavidin, 1000x×)15 μLTube, dilute before use2–8 °C
TMB15 mLTube, ready to use2–8 °C
Stop Solution15 mLTube, ready to use2–8 °C

Validation Data

Figure 1. AAV2 capsid titration curve. The table shows the standard concentrations of AAV2 capsids (vp/mL) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9993) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.

AAV2 capsid titration curve

Figure 2. AAV5 capsid titration curve. Standard concentrations of AAV5 capsids (vp/mL) vs. OD450 readings, with R² = 0.9995 confirming the assay's sensitivity and accuracy across the tested range.

AAV5 capsid titration curve

Figure 3. AAV6 capsid titration curve. Standard concentrations of AAV6 capsids (vp/mL) vs. OD450 readings, with R² = 0.9999 confirming the assay's sensitivity and accuracy.

AAV6 capsid titration curve

Figure 4. AAV8 capsid titration curve. Standard concentrations of AAV8 capsids (vp/mL) vs. OD450 readings, with R² = 0.9998 confirming the assay's sensitivity and accuracy.

AAV8 capsid titration curve

Figure 5. AAV9 capsid titration curve. Standard concentrations of AAV9 capsids (vp/mL) vs. OD450 readings, with R² = 0.9998 confirming the assay's sensitivity and accuracy.

AAV9 capsid titration curve