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TrueX® - AAV8 Serotype Titration Kit (ELISA)
GeneMedi's TrueX® - AAV8 Serotype Titration Kit (ELISA) provides precise and reliable quantification of AAV8 viral particles through a specialized sandwich ELISA format.
This kit utilizes a recombinant antibody that recognizes a conformational epitope present on assembled AAV8 capsids, ensuring the accurate detection of the AAV8 serotype. Designed for exceptional performance in research settings, it delivers reproducible results with high sensitivity for AAV8 analysis. The kit includes all necessary components for efficient titration and is optimized for ease of use while maintaining strict quality standards for AAV-based gene and cell therapy development.
TrueX® AAV Titration & Quantification Solution
COAOrder information
| Product Type | Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|---|
| AAV8 | GMV-TrueX-ELISA-8-1-96-1p | TrueX® - AAV8 Serotype Titration Kit (ELISA) | 1 plate(96 wells) |
Description
| Catalog number | GMV-TrueX-ELISA-8-1-96-1p |
| Product name | TrueX® - AAV8 Serotype Titration Kit (ELISA) |
| Contents | 12 x 8 Determinations |
| Storage | 2-8 °C |
Key components
| Components | Size | Format | Storage |
|---|---|---|---|
| MTP (Microtiter Plate) | 12 x 8-well strips | Vacuum packing with desiccant bag | 2-8°C |
| Assay Buffer (10x) | 50 mL | Tube, dilute before use | 2-8°C |
| Biotin conjugated anti-AAV antibody (100x) | 150 μL | Tube, dilute before use | 2-8°C |
| HRP-SA (HRP labelled streptavidin, 1000x) | 15 μL | Tube, dilute before use | 2-8°C |
| TMB | 15 mL | Tube, ready to use | 2-8°C |
| Stop Solution | 15 mL | Tube, ready to use | 2-8°C |
Validation Data
Figure 4. AAV8 capsid titration curve generated using the GenMedi AAV Titration ELISA kit.
The table shows the standard concentrations of AAV 8 capsids (vp/ml) and their corresponding optical density (OD450) readings. The graph plots OD450 values against capsid concentrations, demonstrating a strong correlation (R² = 0.9998) between capsid quantity and absorbance, confirming the assay's sensitivity and accuracy.
