SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) (Cat No.:GM-NK-Cell-iso-kit)

Achieve superior research results with the SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit. Designed for high-efficiency Human NK Cell purification from PBMC, this kit utilizes a specialized Column-based method with Magnetic Beads to ensure exceptional recovery and purity (up to 94.3%)(1). Our Untouched (Negative) selection strategy leaves target NK cells completely antibody- and bead-free, preserving their native state for functional assays. Ideal for immunology and cytotoxicity studies requiring High Purity and zero activation.
SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
Check GeneMedi's SOLIDEX™-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GM-NK-Cell-iso-kit-10T SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 10 Tests 190
GM-NK-Cell-iso-kit-50T SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 50 Tests 570
GM-NK-Cell-iso-kit-100T SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 100 Tests 1004
GM-NK-Cell-iso-kit-750T SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 750 Tests

Description


Cat No. GM-NK-Cell-iso-kit
Product Name SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
Description SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
Application SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-NK-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Cell population NK Cell
Storage Store at 2℃-8℃

Application

  • Functional Assays: Ideal for cytotoxicity studies (e.g., ADCC assays) as cells remain unaltered(2).
  • Phenotyping: Flow cytometry analysis of surface markers without interference from isolation antibodies.
  • Cell Therapy Research: Isolation of NK cells for expansion or CAR-NK development.
  • Molecular Biology: RNA/DNA extraction from pure NK populations.

Protocol

  1. Preparation: Resuspend PBMCs in separation buffer.
  2. Labeling: Add the Isolation Cocktail and incubate for 15 minutes at 2-8°C(3).
  3. Washing: Wash cells with buffer to remove unbound antibodies(4).
  4. Bead Incubation: Add Anti-Biotin Nanobeads and incubate for 15 minutes at 2-8°C(5).
  5. Separation: Pass the suspension through the LS Column; collect the flow-through (enriched NK cells)(6).

Validation Data

Efficient Isolation of NK Cells Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)

Catalog No.GM-NK-Cell-iso-kit
To determine the purity of isolated NK cells, CD56+ NK cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with CD3-APC and CD56-PE antibodies and subsequently analyzed by flow cytometry. Results demonstrated a remarkable increase in CD56+ NK cell purity from 8.19% pre-isolated to 94.3% post-isolated, confirming highly effective NK cell enrichment.

Figure 5. Efficient Isolation of NK Cells Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The non-NK cells were labeled with biotinylated antibodies. After incubating with anti-biotin magnetic nanobeads, these cells were depleted by cell isolation column. The NK cells were harvested by collecting the flowthrough component. The NK cells in pre-isolated human PBMCs. NK cells with high purity were enriched.

ISOEx Cell Isolation Kit & Nanobeads validation
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High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)

Catalog No.GM-Tg-hg-NK-Cell-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications.

Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.

ISOEx Cell Isolation Kit & Nanobeads validation
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Click to get more Data / Case study about the product.


Background

Why Choose SOLIDEX™-ISOEx?

  • Untouched Purity: Delivers high purity (validated data shows increase from ~8% to >94%) while keeping NK cells free of labels.
  • Preserved Functionality: The negative selection strategy ensures zero activation, maintaining the cell's native killing capacity.
  • Column-Based Efficiency: The column amplifies the magnetic field, ensuring superior depletion of non-target cells compared to column-free methods.
  • Fast Workflow: Optimized protocol with short incubation times allowing rapid isolation.

FAQ

What are the sample requirements? Can I use frozen PBMCs?
The kit is optimized for human PBMCs. Both fresh and cryopreserved PBMCs can be used. However, for frozen samples, ensure high viability (>90%) and remove clumps/debris with a strainer before labeling to prevent column clogging.