SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) (Cat No.:GM-NK-Cell-iso-kit)

Achieve superior research results with the SOLIDEX<sup>®</sup>-ISOEx Untouched Human NK Cell Isolation Kit. Designed for high-efficiency Human NK Cell purification from PBMC, this kit utilizes a specialized Column-based method with Magnetic Beads to ensure exceptional recovery and purity (up to 94.3%)(1). Our Untouched (Negative) selection strategy leaves target NK cells completely antibody- and bead-free, preserving their native state for functional assays. Ideal for immunology and cytotoxicity studies requiring High Purity and zero activation.


SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
Check GeneMedi's SOLIDEX™-ISOEx Cell Isolation Kit & Nanobeads


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SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)

Order information


Catalog No. Product Name Size Price(In USD)
GM-NK-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 10 Tests 190
GM-NK-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 100 Tests 1004
GM-NK-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) 750 Tests

Description


Cat No. GM-NK-Cell-iso-kit
Product Name SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
Application SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-NK-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Cell population NK Cell
Storage Store at 2°C-8°C

Application

<ul> <li><strong>Functional Assays:</strong> Ideal for cytotoxicity studies (e.g., ADCC assays) as cells remain unaltered(2).</li> <li><strong>Phenotyping:</strong> Flow cytometry analysis of surface markers without interference from isolation antibodies.</li> <li><strong>Cell Therapy Research:</strong> Isolation of NK cells for expansion or CAR-NK development.</li> <li><strong>Molecular Biology:</strong> RNA/DNA extraction from pure NK populations.</li> </ul>


Protocol

<ol> <li><strong>Preparation:</strong> Resuspend PBMCs in separation buffer.</li> <li><strong>Labeling:</strong> Add the Isolation Cocktail and incubate for 15 minutes at 2-8°C(3).</li> <li><strong>Washing:</strong> Wash cells with buffer to remove unbound antibodies(4).</li> <li><strong>Bead Incubation:</strong> Add Anti-Biotin Nanobeads and incubate for 15 minutes at 2-8°C(5).</li> <li><strong>Separation:</strong> Pass the suspension through the LS Column; collect the flow-through (enriched NK cells)(6).</li> </ol>


Validation Data

<div class="container"> <div class="layui-row"> <div class="layui-col-xs6 layui-col-md6"> <p style="font-size: 13px;margin-right: 40px;"><strong>High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX<sup>®</sup>-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)</strong> </p> <p style="font-size: 13px;margin-right: 40px;"><strong>Catalog No.GM-Tg-hg-NK-Cell-iso-kit</strong><br>Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX<sup>®</sup>-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX<sup>®</sup>-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications. </p> <p style="font-size: 13px;margin-right: 40px;"><strong>Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX<sup>®</sup>-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). </strong> The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.</p> </div> <div class="layui-col-xs12 layui-col-md6"> <div class="img-wrapper"> <div class="img-wrapper"><img src="https://www.genemedi.net/images/solidex-antibodies-6.webp&quot; style="max-width: 95%; margin-right: 40px;" alt="ISOEx Cell Isolation Kit & Nanobeads validation" loading="lazy" class="image-zoomable"> </div> <div class="tooltip">Click to Zoom</div> </div> </div> </div> </div>


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Background

Why Choose SOLIDEX®-ISOEx?

  • Untouched Purity: Delivers high purity (validated data shows increase from ~8% to >94%) while keeping NK cells free of labels.
  • Preserved Functionality: The negative selection strategy ensures zero activation, maintaining the cell's native killing capacity.
  • Column-Based Efficiency: The column amplifies the magnetic field, ensuring superior depletion of non-target cells compared to column-free methods.
  • Fast Workflow: Optimized protocol with short incubation times allowing rapid isolation.

FAQ

<details> <summary>What are the sample requirements? Can I use frozen PBMCs?</summary> <div class="faq-content">The kit is optimized for human PBMCs. Both fresh and cryopreserved PBMCs can be used. However, for frozen samples, ensure <strong>high viability (&gt;90%)</strong> and remove clumps/debris with a strainer before labeling to prevent column clogging.</div> </details>