SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) (Cat No.:GM-CD4-T-Cell-iso-kit-CF)


SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) is a Indirect CD4+ T Cell Isolation Kit (Column-free, Non-releasable)
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Order information


Catalog No. Product Name Size Price(In USD)
GM-CD4-T-Cell-iso-kit-CF-10T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) 10 Tests 190
GM-CD4-T-Cell-iso-kit-CF-50T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) 50 Tests 570
GM-CD4-T-Cell-iso-kit-CF-100T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) 100 Tests 1004
GM-CD4-T-Cell-iso-kit-CF-750T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) 750 Tests

Description


Cat No. GM-CD4-T-Cell-iso-kit-CF
Product Name SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free)
Description SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) is a Indirect CD4+ T Cell Isolation Kit (Column-free, Non-releasable)
Application SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free) is used for Negative CD4+ T Cell Isolation (Untouched), with the Key Features, such as Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-CD4-T-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-CF-100μL/0.5mL/1mL/7.5mL: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Free)-100μL/0.5mL/1mL/7.5mL
Labeling Type Indirect
Bead Type Non-releasable
Format Column-free
Key feature Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications
Cell population CD4-T Cell
Storage Store at 2°C–8°C

Validation Data

Superior Isolation of High-Purity CD4+ T Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free)

Catalog No. GM-CD4-T-Cell-iso-kit-CF
To evaluate the isolation efficiency of the SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free), CD4+ T cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. In this column-free protocol, non-target cells were labeled with a biotinylated antibody cocktail followed by magnetic beads. The labeled cells were then sequestered by a magnetic separator, allowing the highly purified, untouched CD4+ T cells to be recovered directly from the supernatant. Cell fractions were stained with CD3-APC and CD4-PE for flow cytometric analysis. The frequency of CD4+ T cells demonstrated a substantial increase from 18.4% in the pre-isolation sample to 95.1% in the post-isolation fraction. These results confirm that the column-free system provides effective depletion of non-target populations, yielding high-purity CD4+ T cells while maintaining their native state and maximal viability.

Figure 18. Rapid isolation and high-purity enrichment of Human CD4+ T Cells Using SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-free). Representative flow cytometry plots showing the frequency of CD4+ T cells in hPBMCs before isolation (18.4%) and after enrichment via column-free negative selection (95.1%).

ISOEx Cell Isolation Kit & Nanobeads validation
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