SOLIDEX®-ISOEx L Column (Cat No.:GMP-ISOEx-Column-L)

GeneMedi's SOLIDEX®-ISOEx L Column is developed for the separation of cells labeled with superparamagnetic nanobeads, enabling rapid and gentle separation of flowing cells while ensuring stable cell status and maintenance of activity.

This product is compatible with all SOLIDEX®-ISOEx Nanobeads in GeneMedi's column-based (ISOEx-Enhanced) system. Due to the small size of SOLIDEX®-ISOEx Nanobeads (Column-Based), a high-intensity magnetic field is required to retain the labeled cells. The column needs to be placed in a magnetic rack, where a strong magnetic field separates the target cells. The SOLIDEX®-ISOEx L Column is compatible with all magnetic separation stands and racks on the market.

When used in combination with SOLIDEX®-ISOEx Nanobeads, it can yield target cells with high purity, high recovery rate, and minimal labeling. The separated cells maintain good biological functions and can directly proceed to downstream experimental processes, such as flow cytometry analysis, cell culture, or functional assays. Furthermore, this product ensures experimental consistency and performance stability while, furthermore, this product ensures performance stability while providing excellent cost-effectiveness, helping users control experimental costs.

When used in conjunction with a SOLIDEX®-ISOEx Magnetic Separator (such as the MX1 or MX8), the optimized matrix within the column generates a high-gradient magnetic field powerful enough to efficiently capture cells even with minimal SOLIDEX®-ISOEx nanobead labeling. It is suitable for a diverse array of applications, ranging from small-scale research to the positive selection of rare cells and the depletion of strongly labeled cells.

Product Features

  • Time-saving & Cost-efficient

  • Quick & Easy operation

  • High compatibility: compatible with various brands of magnetic beads and magnetic racks

  • High purity, high recovery rate, high viability

  • Batch consistency

Important Notes:

A. Before adding the next buffer, ensure the previous buffer has completely flowed through (indicated by the absence of continuous dripping from the bottom of the separation column), but do not allow the column to run dry.

B. Column capacity may decrease when separating cells larger than lymphocytes.

C. Sterile packaging, disposable only.

D. For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


SOLIDEX®-ISOEx L Column is developed for the isolation of cells labeled with superparamagnetic nanobeads, which can realize the rapid and gentle isolation of flowing cells and ensure the stability and activity maintenance of cells.
Check GeneMedi's SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GMP-ISOEx-Column-L SOLIDEX®-ISOEx L Column 25 pieces 357

Description


Cat No. GMP-ISOEx-Column-L
Product Name SOLIDEX®-ISOEx L Column
Description SOLIDEX®-ISOEx L Column is developed for the isolation of cells labeled with superparamagnetic nanobeads, which can realize the rapid and gentle isolation of flowing cells and ensure the stability and activity maintenance of cells.
Application SOLIDEX®-ISOEx L Column have been developed for positive selection, negative selection and depletion of human cells in combination with the SOLIDEX®-ISOEx Nanobeads.
Component Box of 25 individually packaged SOLIDEX®-ISOEx L Column and plungers, sterile.
Format Column-based
Capacity Labeled cells: 1×105 - 1×108;
Total cells: 1×107 - 2×109 with manual uses
Storage Store columns dry at room temperature and protected from light.
Compatible Equipment SOLIDEX®-ISOEx MX1 Magnetic Separator

Validation Data

Effective Performance of GeneMedi's SOLIDEX<sup>®</sup>-ISOEx L Column for Cell isolation
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Effective Performance of GeneMedi's SOLIDEX®-ISOEx L Column for Cell isolation

Catalog No. GMP-ISOEx-Column-1

Comparative evaluation of the overall performance of GeneMedi's SOLIDEX®-ISOEx L Column and Company M's Column in the isolation of TCR alpha-beta (abT)+ cells. The Company M's Column yielded slightly higher numbers of bound cells and flow-through cells. Given the minor magnitude of this difference and the absence of coordinated advantages across multiple key performance parameters, it may arise from inherent variability in the isolation procedure.

The positive cell populations obtained from both columns exhibited comparable purity and cell viability, both meeting expected standards. In summary, the GeneMedi SOLIDEX®-ISOEx L Column demonstrates equivalent cell isolation efficacy to the Company M's Column. The SOLIDEX®-ISOEx L Column provides a reliable and effective isolation solution.

Fig. Comparison of cell isolation performance between the two columns.
As shown, both columns achieved high purity of target cells. The Company M's Column (A) yielded slightly higher absolute numbers of bound cells and flow-through cells, but showed no significant differences in cell purity or viability compared to the GeneMedi 's SOLIDEX®-ISOEx L Column (B).

GeneMedi SOLIDEX<sup>®</sup>-ISOEx L Column: High-Efficiency Cell isolation Performance and Batch Stability
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Effective Performance of GeneMedi's SOLIDEX®-ISOEx L Column for Cell isolation

Catalog No. GMP-ISOEx-Column-1

GeneMedi's SOLIDEX®-ISOEx L Column exhibits excellent cell isolation performance and stable batch consistency. Taking the sorting of CD3+ T cells from cryopreserved PBMCs as an example, the A and B batches (n=2) of this column achieved post-sorting purity, viability, and recovery rates for CD3+ T cells all exceeding 90%. Experimental results demonstrate that GeneMedi's SOLIDEX®-ISOEx L Column performs comparably to the isolation column from Company M, providing a reliable and efficient solution for cell isolation.

Fig. GeneMedi's SOLIDEX®-ISOEx L Column demonstrates superior cell sorting efficacy.
GeneMedi's SOLIDEX®-ISOEx L Column shows no significant differences in cell isolation effects compared to the isolation column from Company M, where A and B represent different batches of isolation columns. Experimental data indicate that the purity, viability, and recovery rates of cells post-processing with GeneMedi's isolation column are all greater than 90%.


Click to get more Data / Case study about the product.


FAQ

Q1: What is the main difference between the SOLIDEX®-ISOEx M Column and the SOLIDEX®-ISOEx L Column? How should I choose?
The primary difference lies in their maximum cell loading capacity.
  • SOLIDEX®-ISOEx M Column: Designed for small-scale isolations (up to 2×108 total cells and 2×107 labeled target cells).
  • SOLIDEX®-ISOEx L Column: Designed for large-scale isolations (up to 2×109 total cells and 2×108 labeled target cells).
Select the column based on your starting cell number and expected target cell yield.


Q2: What isolation strategies are compatible with these columns?
When used with the SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads, both columns fully support:
  • Positive Selection
  • Negative Selection
  • Depletion
The high-gradient magnetic field ensures optimal retention of labeled cells while allowing unlabeled cells to flow through smoothly.


Q3: Can the SOLIDEX®-ISOEx columns be washed and reused?
No, they are intended for single use only.

Reusing columns poses a high risk of cross-contamination. Additionally, repeated washing alters the column's internal matrix, significantly compromising purity, recovery rate, and flow rate—leading to unreliable results.


Q4: Are the columns sterile? Do I need to sterilize them before use?
Yes, the columns are strictly sterilized and individually packaged. They are ready to use directly in a laminar flow hood or biosafety cabinet without additional sterilization.

However, always equilibrate (rinse) the column with the recommended buffer according to the protocol before applying your cell suspension.


Q5: What should I do if the column flow rate slows down or gets clogged?
Clogging is usually caused by cell clumps or exceeding capacity. We recommend these preventative measures:
  1. Filtration: Always pass your cell suspension through a 30 µm or 70 µm cell strainer before loading.
  2. Nuclease Treatment: If viscosity is high due to free DNA from dead cells, treat the suspension with DNase I.
  3. Capacity Check: Ensure neither the total cell number nor the labeled target cell number exceeds the column's specified limits.


Q6: Are these columns compatible with standard magnetic separators currently on the market?
Yes. The external dimensions of both the M and L Columns are highly compatible with industry standards. They fit perfectly into mainstream magnetic separators of corresponding sizes, allowing seamless integration into your existing workflow without purchasing new equipment.