SOLIDEX™ Pan-T Cell Isolation Solution
Introduction of SOLIDEX™ Pan-T Cell Isolation Solution
Pan-T Cells encompass all T cell subtypes, including helper T cells, cytotoxic T cells, and regulatory T cells. They are core to adaptive immunity, coordinating cell-mediated immunity and humoral immune regulation, playing a crucial role in infection defense, tumor immunity, and autoimmune diseases.
CD3E (CD3 epsilon) is a key subunit of the T cell receptor (TCR) complex, part of the CD3 molecule family (which includes CD3ε, CD3δ, CD3γ, and CD3ζ subunits), and is widely expressed on the surface of all mature T cells, making it a universal marker for Pan-T cells. immunomagnetic cell separation is a commonly used technique for isolating Pan-T cells, utilizing beads coated with antibodies for efficient separation. GeneMedi provides three types of immunomagnetic cell separation: Negative Selection/Untouched, Positive Selection, and Depletion, to meet diverse research and therapeutic needs.
Isolation method of SOLIDEX™ Pan-T Cell Isolation Solution
- Positive Selection
- Negative Selection
- Depletion
Research Applications:
Through CD3 immunomagnetic cell separation, T cell cytotoxicity, activation processes, HIV infectivity, signaling mechanisms, and surface marker expression can be studied. This method is also used for isolating specific T cell populations, such as T cells secreting specific cytokines, or for analyzing antigen-specific TCR repertoires, helping to further understand immune responses and T cell-mediated immune evasion mechanisms.
Clinical Applications:
In clinical settings, positive selection optimizes CD3+ T cell isolation and culture techniques, particularly providing essential support for clinical research in CAR-T cell therapy. These studies have laid the foundation for the implementation of CAR-T cell treatments.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T87075-iso-nanoIMB | Human CD3E Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T87075-iso-nanoIMB-CF | Human CD3E Nano-mag Beads (Column-Free) | Details |
| GM-Tg-hg-T87075-iso-rel-kit | Indirect Releasable Human CD3E+ Pan-T Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T87075-iso-rel-kit-CF | Indirect Releasable Human CD3E+ Pan-T Cell Isolation Kit (Column-Free) | Details |
| GM-Tg-hg-T87075-iso-kit | Indirect Human CD3E+ Pan-T Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T87075-iso-kit-CF | Indirect Human CD3E+ Pan-T Cell Isolation Kit (Column-Free) | Details |
Research Applications:
Negative selection removes non-T cell populations, enriching for pure T cells, suitable for more precise T cell subset isolation. Researchers can further isolate specific T cell subsets, such as naive T cells, memory T cells, or activated T cells, which is crucial for studying T cell development, immune memory, and dynamic immune responses.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Pan-T-Cell-iso-kit | Untouched Pan-T Cell Isolation Kit (Column-Based) | Details |
| GM-Pan-T-Cell-iso-kit-CF | Untouched Pan-T Cell Isolation Kit (Column-Free) | Details |
Research Applications:
The depletion method, by depleting T cells, allows for the study of the function of other immune cells, especially innate immune cells (such as NK cells) and their anti-infective roles without T cell interference. Additionally, by removing T cells, researchers can focus on studying the immune functions of non-T cell populations and their roles in disease.
Clinical Applications:
Studies show that using CD3/CD19 depletion grafts with RIC treatment leads to delayed reconstitution of T cells and B cells, while NK cell reconstitution occurs earlier and more rapidly. Moreover, when comparing CD34+ selection grafts with CD3/CD19 depletion grafts for immune reconstitution in pediatric acute leukemia patients undergoing haploidentical stem cell transplantation, CD3/CD19 depletion grafts were found to better promote immune reconstitution. In clinical applications involving NK cells, selecting CD56+ cells after depleting CD3+ cells effectively enriches clinical-grade NK cells, providing support for implementing CAR-NK cell strategies.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T87075-dep-nanoIMB | Human CD3E+ Pan-T Cell Depletion Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T87075-dep-kit | Indirect Human CD3E+ Pan-T Cell Depletion Kit (Column-Based) | Details |
