SOLIDEX™-ISOEx Pan-T Cell Isolation Solution
Introduction of SOLIDEX™-ISOEx Pan-T Cell Isolation Solution
Pan-T Cells encompass all T cell subtypes, including helper T cells, cytotoxic T cells, and regulatory T cells. They are core to adaptive immunity, coordinating cell-mediated immunity and humoral immune regulation, playing a crucial role in infection defense, tumor immunity, and autoimmune diseases.
CD3E (CD3 epsilon) is a key subunit of the T cell receptor (TCR) complex, part of the CD3 molecule family (which includes CD3ε, CD3δ, CD3γ, and CD3ζ subunits), and is widely expressed on the surface of all mature T cells, making it a universal marker for Pan-T cells. immunomagnetic cell separation is a commonly used technique for isolating Pan-T cells, utilizing beads coated with antibodies for efficient separation. GeneMedi provides three types of immunomagnetic cell separation: Negative Selection/Untouched, Positive Selection, and Depletion, to meet diverse research and therapeutic needs.
Isolation method of SOLIDEX™-ISOEx Pan-T Cell Isolation Solution
- Positive Selection
- Negative Selection
- Depletion
Research Applications:
Through CD3 immunomagnetic cell separation, T cell cytotoxicity, activation processes, HIV infectivity, signaling mechanisms, and surface marker expression can be studied. This method is also used for isolating specific T cell populations, such as T cells secreting specific cytokines, or for analyzing antigen-specific TCR repertoires, helping to further understand immune responses and T cell-mediated immune evasion mechanisms.
Clinical Applications:
In clinical settings, positive selection optimizes CD3+ T cell isolation and culture techniques, particularly providing essential support for clinical research in CAR-T cell therapy. These studies have laid the foundation for the implementation of CAR-T cell treatments.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T87075-iso-nanoIMB | SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-based) | Details |
| GM-Tg-hg-T87075-iso-nanoIMB-CF | SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-free) | Details |
| GM-Tg-hg-T87075-iso-rel-kit | SOLIDEX™-ISOEx Indirect Releasable Human CD3+ Pan-T Cell Isolation Kit (Column-based) | Details |
| GM-Tg-hg-T87075-iso-rel-kit-CF | SOLIDEX™-ISOEx Indirect Releasable Human CD3+ Pan-T Cell Isolation Kit (Column-free) | Details |
| GM-Tg-hg-T87075-iso-kit | SOLIDEX™-ISOEx Indirect Human CD3+ Pan-T Cell Isolation Kit (Column-based) | Details |
| GM-Tg-hg-T87075-iso-kit-CF | SOLIDEX™-ISOEx Indirect Human CD3+ Pan-T Cell Isolation Kit (Column-free) | Details |
Research Applications:
Negative selection removes non-T cell populations, enriching for pure T cells, suitable for more precise T cell subset isolation. Researchers can further isolate specific T cell subsets, such as naive T cells, memory T cells, or activated T cells, which is crucial for studying T cell development, immune memory, and dynamic immune responses.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Pan-T-Cell-iso-kit | SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-based) | Details |
| GM-Pan-T-Cell-iso-kit-CF | SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free) | Details |
Research Applications:
The depletion method, by depleting T cells, allows for the study of the function of other immune cells, especially innate immune cells (such as NK cells) and their anti-infective roles without T cell interference. Additionally, by removing T cells, researchers can focus on studying the immune functions of non-T cell populations and their roles in disease.
Clinical Applications:
Studies show that using CD3/CD19 depletion grafts with RIC treatment leads to delayed reconstitution of T cells and B cells, while NK cell reconstitution occurs earlier and more rapidly. Moreover, when comparing CD34+ selection grafts with CD3/CD19 depletion grafts for immune reconstitution in pediatric acute leukemia patients undergoing haploidentical stem cell transplantation, CD3/CD19 depletion grafts were found to better promote immune reconstitution. In clinical applications involving NK cells, selecting CD56+ cells after depleting CD3+ cells effectively enriches clinical-grade NK cells, providing support for implementing CAR-NK cell strategies.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T87075-dep-nanoIMB | SOLIDEX™-ISOEx Human CD3+ Pan-T Cell Depletion Nanobeads (Column-based) | Details |
| GM-Tg-hg-T87075-dep-kit | SOLIDEX™-ISOEx Indirect Human CD3+ Pan-T Cell Depletion Kit (Column-based) | Details |
Validation Data
Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Based)
Catalog No.GM-Tg-hg-T87075-iso-nanoIMB
To assess the purity of isolated T cells, CD3+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with APC-conjugated CD3 antibody and propidium iodide (PI), followed by flow cytometric analysis. The results demonstrated a significant increase in CD3+ T cell purity from 52.6% pre-isolated to 95.0% post-isolated, confirming successful T cell isolation.
Figure 7. Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Based). The T cells were labeled with SOLIDEX™-ISOEx Nanobeads conjugated with anti-CD3 antibody. And then these cells were enriched by using cell isolation column. The T cells in pre-isolated human PBMCs. T cells with high purity and motility were enriched.
Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Free)
Catalog No.GM-Tg-hg-T87075-iso-nanoIMB-CF
To evaluate the purity of isolated T cells, CD3+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with APC-conjugated CD3 antibody and analyzed by flow cytometry. The CD3+ T cell purity demonstrated a substantial increase from 65.9% pre-separation to 98.6% post-separation, confirming highly effective T cell isolation.
Figure 8. Efficient Isolation of Pan-T Cells Using SOLIDEX™-ISOEx Human CD3 Isolation Nanobeads (Column-Free). The T cells were labeled with SOLIDEX™-ISOEx Nanobeads conjugated with anti-CD3 antibody. And then these cells were enriched by using magnetic separator. The T cells in pre-isolated human PBMCs. T cells with high purity and motility were enriched.
High-purity isolation of Pan-T cells from human PBMC using the SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-based)
Catalog No. GM-Pan-T-Cell-iso-kit
In this experiment, the SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-based) was used to isolate and purify Pan-T cells from human peripheral blood mononuclear cells (PBMC). First, non-Pan-T cells were specifically labeled with a biotin-conjugated antibody cocktail (Antibody Cocktail), followed by incubation with anti-biotin nano-magnetic beads. When the cell suspension passed through the separation column, the magnetically labeled non-target cells were retained in the column, while the unlabeled, untouched Pan-T cells flowed out with the elution buffer. Data showed that the proportion of Pan-T cells increased significantly from 58.9% before isolation to 96.1% after isolation. The experimental results demonstrate that this kit effectively removes various types of non-Pan-T cells, yielding high-purity Pan-T cells that retain their original biological activity.
Figure 12. High-purity isolation of Pan-T cells from human PBMC using the SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-based). Flow cytometry analysis was performed on the cell populations before and after isolation. The results confirm that this method successfully yields high-purity Pan-T cells.
