SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) (Cat No.:GM-Pan-T-Cell-iso-kit)

Achieve superior purity with GeneMedi’s SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit. Optimized for Human Pan-T Cell isolation from PBMC, this Column-based system utilizes a negative selection strategy to deliver truly "untouched" cells. Our biotin-conjugated antibody cocktail and Magnetic Beads remove non-T cells, ensuring the target CD3+ cells remain free of antibodies and bead residues. With High Purity (>95%) and zero activation, these cells are ideal for downstream functional assays.

SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is a Indirect CD3+ Pan-T Cell Isolation Kit (Column-based, Non-releasable)
Check GeneMedi's SOLIDEX™-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GM-Pan-T-Cell-iso-kit-10T SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 10 Tests 190
GM-Pan-T-Cell-iso-kit-50T SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 50 Tests 570
GM-Pan-T-Cell-iso-kit-100T SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 100 Tests 1004
GM-Pan-T-Cell-iso-kit-750T SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) 750 Tests

Description


Cat No. GM-Pan-T-Cell-iso-kit
Product Name SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based)
Description SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is a Indirect CD3+ Pan-T Cell Isolation Kit (Column-based, Non-releasable)
Application SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) is used for Negative CD3+ Pan-T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-Pan-T-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-200μL/1ml/2ml/15ml: SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based)-200μL/1ml/2ml/15ml
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Cell population CD3+ Pan-T Cell
Storage Store at 2℃-8℃

Application

  • In vitro T cell activation and expansion: Perfect for functional studies as cells are unstimulated.
  • Flow Cytometry analysis: Cells have no surface antibodies, allowing immediate staining with structural integrity.
  • Molecular biology assays: High purity RNA/DNA extraction for sequencing or PCR.
  • Adoptive cell therapy research: Starting material for CAR-T or TCR-T development.

Protocol

  1. Preparation: Isolate and count PBMCs; resuspend in separation buffer.
  2. Labeling: Add the Isolation Cocktail to cells and incubate for 5 minutes at room temperature.
  3. Magnetic Binding: Add Anti-biotin Nanobeads and incubate for 10 minutes at 2-8°C.
  4. Separation: Apply cell suspension to an LS Column on a magnetic separator. Collect the flow-through (this contains your enriched Pan-T cells).

Validation Data

High-purity isolation of CD3+ Pan-T Cells from human PBMC using the SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based)

Catalog No. GM-Pan-T-Cell-iso-kit
In this experiment, the SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based) was used to isolate and purify CD3+ Pan-T Cells from human peripheral blood mononuclear cells (PBMC). First, non-CD3+ Pan-T Cells were specifically labeled with a biotin-conjugated antibody cocktail (Antibody Cocktail), followed by incubation with anti-biotin nano-magnetic beads. When the cell suspension passed through the separation column, the magnetically labeled non-target cells were retained in the column, while the unlabeled, untouched CD3+ Pan-T Cells flowed out with the elution buffer. Data showed that the proportion of CD3+ Pan-T Cells increased significantly from 58.9% before isolation to 96.1% after isolation. The experimental results demonstrate that this kit effectively removes various types of non-CD3+ Pan-T Cells, yielding high-purity CD3+ Pan-T Cells that retain their original biological activity.

Figure 12. High-purity isolation of CD3+ Pan-T Cells from human PBMC using the SOLIDEX™-ISOEx Untouched Human CD3+ Pan-T Cell Isolation Kit (Column-based). Flow cytometry analysis was performed on the cell populations before and after isolation. The results confirm that this method successfully yields high-purity CD3+ Pan-T Cells.

ISOEx Cell Isolation Kit & Nanobeads validation
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Click to get more Data / Case study about the product.


Background

Why Choose SOLIDEX™-ISOEx Untouched Human Pan-T Cell Isolation Kit?

  • Truly Untouched: Uses negative selection to leave target T cells completely free of antibodies and magnetic particles, preserving their native function.
  • High Purity: Validated to achieve >98% purity (compared to ~58% in PBMCs).
  • Fast Workflow: Optimized rapid protocol with only 15 minutes of total incubation time.
  • High Compatibility: Compatible with standard LS columns and magnetic separators.

FAQ

What are the sample requirements?
This kit is optimized for Peripheral Blood Mononuclear Cells (PBMC). Best results are obtained with fresh PBMCs, but carefully cryopreserved PBMCs can also be used.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (negative) selection kit, the CD3+ T cells have no antibodies or beads attached to their surface. All surface epitopes are available for downstream antibody staining.
How does the column-based negative selection work?
Non-T cells (B cells, NK cells, Monocytes, etc.) are magnetically labeled and retained inside the column by the magnetic field. The Pan-T cells pass through the column freely and are collected in the flow-through.
Can the columns be reused?
No. The columns are designed for single-use to ensure sterility and prevent cross-contamination between samples.
What is the advantage of this column-based method over column-free methods?
Column-based separation typically offers higher purity due to the stronger magnetic gradient generated within the column matrix, which more effectively retains the magnetically labeled non-target cells compared to simple tube magnets.
Is this workflow compatible with automation?
Yes, the magnetic separation steps can be adapted to automated liquid handling systems that support magnetic bead separation or automated column separators.