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GeneMedi's SOLIDEX™-ISOEx L Column
GeneMedi's SOLIDEX™-ISOEx L Column is developed for the separation of cells labeled with superparamagnetic nanobeads, enabling rapid and gentle separation of flowing cells while ensuring stable cell status and maintenance of activity.
This product is compatible with all SOLIDEX™-ISOEx Nanobeads in GeneMedi's column-based (ISOEx-Enhanced) system. Due to the small size of SOLIDEX™-ISOEx Nanobeads (Column-Based), a high-intensity magnetic field is required to retain the labeled cells. The column needs to be placed in a magnetic rack, where a strong magnetic field separates the target cells. The SOLIDEX™-ISOEx L Column is compatible with all magnetic separation stands and racks on the market.
When used in combination with SOLIDEX™-ISOEx Nanobeads, it can yield target cells with high purity, high recovery rate, and minimal labeling. The separated cells maintain good biological functions and can directly proceed to downstream experimental processes, such as flow cytometry analysis, cell culture, or functional assays. Furthermore, this product ensures experimental consistency and performance stability while, furthermore, this product ensures performance stability while providing excellent cost-effectiveness, helping users control experimental costs.
Product Features
High Purity & Recovery: Purity of isolated target cells >99%, recovery >90%
Simple & Rapid Operation: Only three steps—loading, elution, and collection; magnetic separation completed in 5 minutes
Cell Safety: Sterile, non-damaging to cells, gentle isolation process
High Compatibility: Compatible with all magnetic stands on the market
Cost-Effective: Economical pricing, stable performance with batch-to-batch consistency, effectively saving experimental time and consumable costs
Important Notes:
A. Before adding the next buffer, ensure the previous buffer has completely flowed through (indicated by the absence of continuous dripping from the bottom of the separation column), but do not allow the column to run dry.
B. Column capacity may decrease when separating cells larger than lymphocytes.
C. Sterile packaging, disposable only.
D. For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Product Information
| Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|
| GMP-ISOEx-Column-1 | SOLIDEX™-ISOEx L Column | 25 piecces/box | 999 |
Product Description
| Catalog No. | GMP-ISOEx-Column-1 |
| Products Name | SOLIDEX™-ISOEx L Column |
| Description | SOLIDEX™-ISOEx L Column is developed for the separation of cells labeled with superparamagnetic nanobeads, which can realize the rapid and gentle separation of flowing cells and ensure the stability and activity maintenance of cells. |
| Format | Column-Based |
| Application | SOLIDEX™-ISOEx LS Column have been developed for positive selection, negative selection and depletion of human cells in combination with the SOLIDEX™-ISOEx Nanobeads. |
| Components | Box of 25 individually packaged SOLIDEX™-ISOEx L Column and plungers, sterile. |
| Capacity | Labeled cells: 1E5 ~ 1E8; Total cells: 1E7~2E9 with manual uses |
| Storage | Store columns dry at room temperature and protected from light. |
Validation Data
Effective Performance of GeneMedi's SOLIDEX™-ISOEx L Column for Cell isolation
Catalog No. GMP-ISOEx-Column-1
Comparative evaluation of the overall performance of GeneMedi's SOLIDEX™-ISOEx L Column and Company M's Column in the isolation of TCR alpha-beta (abT)+ cells. The Company M's Column yielded slightly higher numbers of bound cells and flow-through cells. Given the minor magnitude of this difference and the absence of coordinated advantages across multiple key performance parameters, it may arise from inherent variability in the isolation procedure.
The positive cell populations obtained from both columns exhibited comparable purity and cell viability, both meeting expected standards. In summary, the GeneMedi SOLIDEX™-ISOEx L Column demonstrates equivalent cell isolation efficacy to the Company M's Column. The SOLIDEX™-ISOEx L Column provides a reliable and effective isolation solution.
Fig. Comparison of cell isolation performance between the two columns.
As shown, both columns achieved high purity of target cells. The Company M's Column (A) yielded slightly higher absolute numbers of bound cells and flow-through cells, but showed no significant differences in cell purity or viability compared to the GeneMedi 's SOLIDEX™-ISOEx L Column (B).
Effective Performance of GeneMedi's SOLIDEX™-ISOEx L Column for Cell isolation
Catalog No. GMP-ISOEx-Column-1
GeneMedi's SOLIDEX™-ISOEx L Column exhibits excellent cell isolation performance and stable batch consistency. Taking the sorting of CD3+ T cells from cryopreserved PBMCs as an example, the A and B batches (n=2) of this column achieved post-sorting purity, viability, and recovery rates for CD3+ T cells all exceeding 90%. Experimental results demonstrate that GeneMedi's SOLIDEX™-ISOEx L Column performs comparably to the isolation column from Company M, providing a reliable and efficient solution for cell isolation.
Fig. GeneMedi's SOLIDEX™-ISOEx L Column demonstrates superior cell sorting efficacy.
GeneMedi's SOLIDEX™-ISOEx L Column shows no significant differences in cell isolation effects compared to the isolation column from Company M, where A and B represent different batches of isolation columns. Experimental data indicate that the purity, viability, and recovery rates of cells post-processing with GeneMedi's isolation column are all greater than 90%.
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