SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) (Cat No.:GM-CD8-T-Cell-iso-kit)

Achieve superior purity with the SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit. Utilizing a highly specific Column-based negative selection strategy, this kit effectively depletes non-target cells from Human PBMC using optimized Magnetic Beads.

SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is a Indirect CD8+ T Cell Isolation Kit (Column-based, Non-releasable)
Check GeneMedi's SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GM-CD8-T-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 10 Tests 190
GM-CD8-T-Cell-iso-kit-50T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 50 Tests 570
GM-CD8-T-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 100 Tests 1004
GM-CD8-T-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) 750 Tests

Description


Cat No. GM-CD8-T-Cell-iso-kit
Product Name SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is a Indirect CD8+ T Cell Isolation Kit (Column-based, Non-releasable)
Application SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based) is used for Negative CD8+ T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-CD8-T-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Cell population CD8-T Cell
Storage Store at 2°C–8°C

Application

  • T Cell Cytotoxicity Assays: Isolation of functionally active CTLs for tumor killing or viral clearance studies without pre-activation.
  • Flow Cytometry & Phenotyping: Epitopes remain unblocked for precise surface marker analysis.
  • Single-Cell Sequencing (scRNA-seq): High-quality, stress-free cells ensure accurate transcriptomic data.
  • Adoptive Cell Therapy Research: Source material for CAR-T cell engineering and expansion.

Protocol

  1. Preparation: Prepare a single-cell suspension from cryopreserved or fresh PBMCs.
  2. Labeling: Add the Biotin-Antibody Cocktail (targets non-CD8+ cells) to the cell suspension. Incubate.
  3. Bead Binding: Add Anti-Biotin Microbeads to bind the labeled non-target cells. Incubate.
  4. Separation: Apply the suspension to the separation column placed in a magnetic field.
  5. Elution: Unlabeled CD8+ T cells pass through the column freely and are collected in the flow-through fraction (Untouched).

Validation Data

Efficient Enrichment of High-Purity CD8+ T Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based)

Catalog No. GM-CD8-T-Cell-iso-kit
To evaluate the isolation efficiency of the SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based), CD8+ T cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. Non-target cells were labeled with a biotinylated antibody cocktail and depleted using magnetic beads within a separation column.
Cell fractions were stained with CD3-APC and CD8-PE for flow cytometric analysis. The frequency of CD8+ T cells demonstrated a substantial increase from 22.6% in the pre-isolation sample to 91.9% in the post-isolation fraction. These results confirm that the column-based system provides superior depletion of non-target populations, yielding highly purified "untouched" CD8+ T cells.

Figure 17. Isolation of Human CD8+ T Cells Using SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-based). Representative flow cytometry plots showing the frequency of CD8+ T cells in hPBMCs before isolation (22.6%) and after enrichment via column-based negative selection (91.9%).

ISOEx Cell Isolation Kit & Nanobeads validation
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Background

Why Choose SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit?

  • 100% Untouched Cells: Target CD8+ T cells are never bound by antibodies or beads, preventing receptor blocking and accidental activation.
  • Superior Purity: Column-based separation acts as a physical filter, significantly reducing background contamination compared to column-free methods.
  • High Viability: Gentle isolation preserves the metabolic health of cytotoxic T cells for sensitive functional assays.
  • Fast & Simple: Streamlined protocol allows for isolation in under 45 minutes.

FAQ

What are the sample requirements?
We recommend using fresh or cryopreserved Human PBMCs. For best results, ensure cell viability is >90% before starting. Leukapheresis products are also compatible.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (Negative Selection) kit, the CD8 receptors on the surface of your target cells are free and not blocked by any magnetic beads or antibodies. You can proceed directly to staining.
How does the Column-based separation work?
The column contains a ferromagnetic matrix. When placed in a magnetic field, it amplifies the magnetic force, effectively retaining the magnetically labeled non-target cells. The unlabeled CD8+ T cells simply pass through.
Are the separation columns reusable?
No. The columns are designed for single-use to ensure maximum sterility and prevent cross-contamination between patient samples.
What is the advantage of Column-based over Column-free methods?
Column-based methods typically offer higher purity. The column matrix allows for thorough washing of the retained cells, ensuring that only the specific "Untouched" cells pass through, whereas column-free methods can sometimes suffer from non-specific entrapment or lower purity in complex samples.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.