SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) (Cat No.:GM-Tg-hg-T56365-dep-kit)


SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) is a Indirect B Cell Depletion Kit (Column-based, Non-releasable)
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Order information


Catalog No. Product Name Size Price(In USD)
GM-Tg-hg-T56365-dep-kit-10T SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) 10 Tests 112
GM-Tg-hg-T56365-dep-kit-50T SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) 50 Tests 336
GM-Tg-hg-T56365-dep-kit-100T SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) 100 Tests 591
GM-Tg-hg-T56365-dep-kit-750T SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) 750 Tests

Description


Cat No. GM-Tg-hg-T56365-dep-kit
Product Name SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based)
Description SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) is a Indirect B Cell Depletion Kit (Column-based, Non-releasable)
Application SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) is used for B Cell Depletion, with the Key Features, such as Specific cell depletion; minimal non-specific binding; optimal for complex samples
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-Tg-hg-T56365-Ab-1-Biotin-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Anti- Human CD19+ B Cell monoclonal antibody(mAb) (Biotin)-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-100μL/0.5mL/1mL/7.5mL: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-100μL/0.5mL/1mL/7.5mL
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Specific cell depletion; minimal non-specific binding; optimal for complex samples
Cell population B Cell
Storage Store at 2°C–8°C

Validation Data

Efficient Depletion of Human B Cells Using the SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based)

Catalog No. GM-Tg-hg-T56365-dep-kit
To evaluate the depletion efficiency of the SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based), human PBMCs were incubated and labeled with Biotin-CD19 antibodies and anti-biotin nanobeads, followed by magnetic column separation. The unlabeled target cells passed through the separation column and were collected, while the magnetically labeled CD19+ B cells were retained within the column. Following the magnetic separation, both pre- and post-depletion cell samples were analyzed by flow cytometry. The flow cytometry plots demonstrated that the initial proportion of B cells in the pre-depletion PBMC sample was 10.1%, whereas the residual B cell proportion in the flow-through fraction after efficient depletion plummeted to 0.091%. The logarithmic depletion for this process reached 2.14. This indicates that the product is capable of highly efficient depletion of non-target B cells.

Figure 23. Efficient depletion of human B cells using the SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based). In this experiment, CD19+ B cells were magnetically sorted and depleted from PBMCs. The results show a significant decrease in the proportion of CD19+ B cells in the sample, dropping from 10.1% pre-depletion to 0.091% after depletion.

ISOEx Cell Isolation Kit & Nanobeads validation
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