SOLIDEX™-ISOEx NK Cell Isolation Solution
Introduction of SOLIDEX™-ISOEx NK Cell Isolation Solution
Natural Killer (NK) Cells are core effector cells of the innate immune system, capable of killing virus-infected cells or cancer cells without prior sensitization. They play a crucial role in antiviral defense, antitumor immunity, and transplant immunity. Key surface markers of NK cells include CD56 (Neural Cell Adhesion Molecule, NCAM) and CD16 (FcγRIII, low-affinity Fc receptor).
CD56 promotes the adhesion and recognition of target cells, enhancing cytotoxic activity. Magnetic bead sorting is a commonly used technique for isolating NK cells, utilizing beads coated with antibodies to achieve efficient separation. GeneMedi provides three types of magnetic bead selection: Negative Selection/Untouched, Positive Selection, and Depletion, to meet various research and therapeutic needs.
Isolation method of SOLIDEX™-ISOEx NK Cell Isolation Solution
- Positive Selection
- Negative Selection
- Depletion
Research Applications:
Positive selection is used to enrich CD56+ NK cells and is widely applied in research on the development, proliferation, cytotoxic activity, and immune functions of NK cells. Using CD56 magnetic beads, researchers can deeply explore the key roles of NK cells in immune surveillance, particularly in cancer immunotherapy.
Clinical Applications:
In immunotherapy, positive selection is used for the enrichment of clinical-grade NK cells. After removing CD3+ T cells, CD56 magnetic beads are employed to isolate high-purity NK cells. Additionally, positive selection is also used in the preparation of CAR NK cell strategies. Studies have shown that CAR-NK cells (expressing anti-CD123 CAR) specifically kill CD123-positive tumor cells.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T86323-iso-nanoIMB | SOLIDEX™-ISOEx Human NCAM1 (CD56)+ NK Cell Isolation Nanobeads (Column-based) | Details |
| GM-Tg-hg-T86323-iso-nanoIMB-CF | SOLIDEX™-ISOEx Human NCAM1 (CD56)+ NK Cell Isolation Nanobeads (Column-free) | Details |
| GM-Tg-hg-T86323-iso-rel-kit | SOLIDEX™-ISOEx Indirect Releasable Human NCAM1 (CD56)+ NK Cell Isolation Kit (Column-based) | Details |
| GM-Tg-hg-T86323-iso-rel-kit-CF | SOLIDEX™-ISOEx Indirect Releasable Human NCAM1 (CD56)+ NK Cell Isolation Kit (Column-free) | Details |
| GM-Tg-hg-T86323-iso-kit | SOLIDEX™-ISOEx Indirect Human NCAM1 (CD56)+ NK Cell Isolation Kit (Column-based) | Details |
| GM-Tg-hg-T86323-iso-kit-CF | SOLIDEX™-ISOEx Indirect Human NCAM1 (CD56)+ NK Cell Isolation Kit (Column-free) | Details |
Research Applications:
Negative selection enriches pure NK cells by removing non-target cells. This technique is used to study NK cell proliferation, cytotoxicity, and their role in tumor immunity. It is also used to analyze the function of NK cell surface receptors and cytokine secretion, helping to elucidate the mechanisms of NK cells in immune responses.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-NK-Cell-iso-kit | SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) | Details |
| GM-NK-Cell-iso-kit-CF | SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) | Details |
Research Applications:
The depletion method involves the removal of NK cells to study their role in immune responses, especially in the context of tumor immune evasion mechanisms. This method aids in exploring the critical functions and regulation of NK cells in immune responses.
Clinical Applications:
Depletion technology is used in clinical research to assess the impact of NK cells on disease progression, particularly in hematological diseases and hematopoietic stem cell transplantation. By depleting NK cells, the function of stem cells can be improved, enhancing the success of transplant treatments.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T86323-dep-nanoIMB | SOLIDEX™-ISOEx Human NCAM1 (CD56)+ NK Cell Depletion Nanobeads (Column-based) | Details |
| GM-Tg-hg-T86323-dep-kit | SOLIDEX™-ISOEx Indirect Human NCAM1 (CD56)+ NK Cell Depletion Kit (Column-based) | Details |
Validation Data
Efficient Isolation of NK Cells Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)
Catalog No.GM-Tg-hg-NK-Cell-iso-kit
To determine the purity of isolated NK cells, CD56+ NK cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with CD3-APC and CD56-PE antibodies and subsequently analyzed by flow cytometry. Results demonstrated a remarkable increase in CD56+ NK cell purity from 8.19% pre-isolated to 94.3% post-isolated, confirming highly effective NK cell enrichment.
Figure 5. Efficient Isolation of NK Cells Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The non-NK cells were labeled with biotinylated antibodies. After incubating with anti-biotin magnetic nanobeads, these cells were depleted by cell isolation column. The NK cells were harvested by collecting the flowthrough component. The NK cells in pre-isolated human PBMCs. NK cells with high purity were enriched.
High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)
Catalog No.GM-Tg-hg-NK-Cell-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications.
Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX™-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.
