SOLIDEX®-ISOEx M Column (Cat No.:GMP-ISOEx-Column-M)

The SOLIDEX®-ISOEx M Column by GeneMedi is specifically developed for the isolation of cells labeled with superparamagnetic nanobeads. It enables the rapid and gentle separation of cells passing through the column, ensuring the stability of cell conditions and the preservation of cell viability. This product is fully compatible with all SOLIDEX®-ISOEx Nanobeads within GeneMedi's column-based (ISOEx-Enhanced) portfolio.

Due to the exceptionally small size of the SOLIDEX®-ISOEx Nanobeads (Column-based), a high-gradient magnetic field is required to retain the magnetically labeled cells. Target cells are isolated via a strong magnetic field when the column is placed within a magnetic stand; notably, the SOLIDEX®-ISOEx M Column is highly compatible with all commercially available magnetic separators.

When used in conjunction with a SOLIDEX®-ISOEx Magnetic Separator (such as the MX1 or MX8), the optimized matrix within the column generates a high-gradient magnetic field powerful enough to efficiently capture cells even with minimal SOLIDEX®-ISOEx nanobead labeling. It is suitable for a diverse array of applications, ranging from small-scale research to the positive selection of rare cells and the depletion of strongly labeled cells.

Product Features

  • Time-saving & Cost-efficient

  • Quick & Easy operation

  • High compatibility: compatible with various brands of magnetic beads and magnetic racks

  • High purity, high recovery rate, high viability

  • Batch consistency

Important Notes

  • Before adding the next buffer, ensure the previous buffer has completely flowed through (indicated by the absence of continuous dripping from the bottom of the isolation column), but do not allow the column to run dry.

  • Column capacity may decrease when separating cells larger than lymphocytes.

  • Sterile packaging, disposable only.

  • For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


SOLIDEX®-ISOEx M Column is developed for the isolation of cells labeled with superparamagnetic nanobeads, which can realize the rapid and gentle isolation of flowing cells and ensure the stability and activity maintenance of cells.
Check GeneMedi's SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GMP-ISOEx-Column-M SOLIDEX®-ISOEx M Column 25 pieces 321

Description


Cat No. GMP-ISOEx-Column-M
Product Name SOLIDEX®-ISOEx M Column
Description SOLIDEX®-ISOEx M Column is developed for the isolation of cells labeled with superparamagnetic nanobeads, which can realize the rapid and gentle isolation of flowing cells and ensure the stability and activity maintenance of cells.
Application SOLIDEX®-ISOEx M Column have been developed for positive selection, negative selection and depletion of human cells in combination with the SOLIDEX®-ISOEx Nanobeads.
Component Box of 25 individually packaged SOLIDEX®-ISOEx M Column and plungers, sterile.
Format Column-based
Capacity Labeled cells: 1×104 - 1×107;
Total cells: 1×106 - 2×108 with manual uses
Storage Store columns dry at room temperature and protected from light.
Compatible Equipment SOLIDEX®-ISOEx LX4 Magnetic Separator

FAQ

Q1: What is the main difference between the SOLIDEX®-ISOEx M Column and the SOLIDEX®-ISOEx L Column? How should I choose?
The primary difference lies in their maximum cell loading capacity.
  • SOLIDEX®-ISOEx M Column: Designed for small-scale isolations (up to 2×108 total cells and 2×107 labeled target cells).
  • SOLIDEX®-ISOEx L Column: Designed for large-scale isolations (up to 2×109 total cells and 2×108 labeled target cells).
Select the column based on your starting cell number and expected target cell yield.


Q2: What isolation strategies are compatible with these columns?
When used with the SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads, both columns fully support:
  • Positive Selection
  • Negative Selection
  • Depletion
The high-gradient magnetic field ensures optimal retention of labeled cells while allowing unlabeled cells to flow through smoothly.


Q3: Can the SOLIDEX®-ISOEx columns be washed and reused?
No, they are intended for single use only.

Reusing columns poses a high risk of cross-contamination. Additionally, repeated washing alters the column's internal matrix, significantly compromising purity, recovery rate, and flow rate—leading to unreliable results.


Q4: Are the columns sterile? Do I need to sterilize them before use?
Yes, the columns are strictly sterilized and individually packaged. They are ready to use directly in a laminar flow hood or biosafety cabinet without additional sterilization.

However, always equilibrate (rinse) the column with the recommended buffer according to the protocol before applying your cell suspension.


Q5: What should I do if the column flow rate slows down or gets clogged?
Clogging is usually caused by cell clumps or exceeding capacity. We recommend these preventative measures:
  1. Filtration: Always pass your cell suspension through a 30 µm or 70 µm cell strainer before loading.
  2. Nuclease Treatment: If viscosity is high due to free DNA from dead cells, treat the suspension with DNase I.
  3. Capacity Check: Ensure neither the total cell number nor the labeled target cell number exceeds the column's specified limits.


Q6: Are these columns compatible with standard magnetic separators currently on the market?
Yes. The external dimensions of both the M and L Columns are highly compatible with industry standards. They fit perfectly into mainstream magnetic separators of corresponding sizes, allowing seamless integration into your existing workflow without purchasing new equipment.