SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) (Cat No.:GM-Tg-hg-TA040-iso-nanoIMB)

Achieve superior isolation of Human CD8+ T Cells (Cytotoxic T Lymphocytes) with SOLIDEX™-ISOEx Nanobeads. Utilizing a high-efficiency Positive Selection strategy, this kit specifically targets CD8 antigens to maximize cell recovery from PBMC or Leukopaks. The Column-based technology combined with advanced Magnetic Beads allows for rigorous washing steps, ensuring High Purity and eliminating non-specific background. These biodegradable nanobeads preserve cell viability, making the isolated cells perfect for tumor killing assays and CAR-T development.

SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) is a Direct CD8+ T Cell Isolation Nanobeads (Column-based, Non-releasable)
Check GeneMedi's SOLIDEX™-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GM-Tg-hg-TA040-iso-nanoIMB-0.1mL SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) 0.1 mL 112
GM-Tg-hg-TA040-iso-nanoIMB-0.5mL SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) 0.5 mL 336
GM-Tg-hg-TA040-iso-nanoIMB-1mL SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) 1 mL 591
GM-Tg-hg-TA040-iso-nanoIMB-7.5mL SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) 7.5 mL

Description


Cat No. GM-Tg-hg-TA040-iso-nanoIMB
Product Name SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based)
Description SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) is a Direct CD8+ T Cell Isolation Nanobeads (Column-based, Non-releasable)
Application SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads (Column-based) is used for Positive CD8+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Labeling Type Direct
Bead Type Non-releasable
Format Column-based
Key feature Fast isolation; high purity; ideal for automated workflows
Cell population CD8-T Cell
Storage Store at 2℃-8℃

Application

  • Cytotoxicity Assays: Isolation of effector cells for tumor cell killing or viral clearance studies.
  • CAR-T Cell Development: Source material for engineering Chimeric Antigen Receptor T cells.
  • Single-Cell Sequencing: High-recovery isolation for TCR repertoire analysis (scRNA-seq).
  • Intracellular Cytokine Staining: Analysis of IFN-gamma, TNF-alpha, or Granzyme B production.

Protocol

  1. Preparation: Prepare a single-cell suspension from Human PBMCs (fresh or frozen).
  2. Labeling: Add CD8 Microbeads directly to the cell suspension. Incubate to bind CD8+ T Cells.
  3. Separation: Place a separation column in the magnetic field and apply the cell suspension.
  4. Washing: The magnetically labeled CD8+ cells are retained in the column; unlabeled cells (CD4+ T cells, B cells, Monocytes) are washed away.
  5. Elution: Remove the column from the magnet and elute the highly purified CD8+ T Cells.

Validation Data

Efficient Isolation of CD8+ T Cells Using SOLIDEX™-ISOEx -ISOEx Human CD8 Isolation Nanobeads (Column-Based)

Catalog No. GM-Tg-hg-TA040-iso-nanoIMB
To determine the purity of isolated CD8+ T cell, CD8+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with CD3-APC and CD8-PE antibodies and subsequently analyzed by flow cytometry. Results demonstrated a remarkable increase in CD8+ T cell purity from 16.6% pre-isolated to 94.6% post-isolated, confirming highly effective CD8+ T cell enrichment.

Figure 10. Efficient Isolation of CD8+ Cells Using SOLIDEX™-ISOEx -ISOEx Human CD8 Isolation Nanobeads (Column-Based). The T cells were labeled with magnetic nanobeads conjugated with anti-CD8 antibody. And then these cells were enriched by using cell isolation column. The T cells in pre-isolated human PBMCs . T cells with high purity were enriched.

ISOEx Immunocytes Cell Isolation Immunomagnetic Beads validation
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Click to get more Data / Case study about the product.


Background

Why Choose SOLIDEX™-ISOEx Human CD8 Isolation Nanobeads?

  • Exceptional Recovery: Positive selection captures the maximum number of target CD8+ cells, essential when working with limited patient samples.
  • Superior Specificity: The column matrix allows for thorough washing, significantly reducing contamination compared to column-free "clump" methods.
  • Biodegradable Nanobeads: The tiny, non-toxic beads degrade naturally and do not over-stimulate the cells, preserving their cytotoxic potential.
  • Rapid Workflow: Isolate cells in approximately 45 minutes with a simple, standardized protocol.

FAQ

What are the sample requirements?
This kit is optimized for fresh or cryopreserved Human PBMCs. It is also fully compatible with Leukapheresis products (Leukopaks). Ensure high cell viability (>90%) and proper DNase treatment if using frozen samples to prevent clumping.
Do the magnetic beads affect cell activation?
No. The SOLIDEX™ nanobeads are biodegradable and extremely small (nano-scale). They are designed to minimize receptor cross-linking and avoid inducing artificial activation, ensuring your CD8+ cells remain in a physiological state for downstream functional assays.
How does the Column-based separation work?
The column contains a matrix of ferromagnetic spheres. When placed in a magnetic field, these spheres amplify the magnetic gradient, effectively trapping the bead-labeled CD8+ cells. This allows non-target cells to be washed away freely.
Are the separation columns reusable?
No. The columns are strictly single-use to ensure sterility and prevent cross-contamination between patient samples or experimental conditions.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection typically yields higher purity. The physical column allows for active washing of the retained cells, removing contaminants that are often trapped non-specifically in the aggregates formed by column-free methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX™-ISOEx column format is compatible with most standard automated magnetic cell separation instruments that utilize column technology.