- • Up to 20% off your first purchase
- • Bulk quantities available
- • Citation Reward application
Supports
- Talk to our Expert:
[email protected] -
Talk to our sales:
[email protected]
Protocol Library Download
 |
 |
SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based)
GeneMedi's SOLIDEX™-ISOEx Cell Isolation Nanobeads products are dedicated to providing efficient and gentle cell separation solutions, achieving high purity and high recovery cell sorting through superparamagnetic nanobead technology, while minimizing interference with cell viability and function. These products are widely applicable to research and clinical applications, such as immune cell therapy, stem cell research, and functional cell analysis.
SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) is a column-based anti-biotin nanobead for in vitro enrichment or depletion of cells labeled with biotinylated antibodies or ligands from fresh/frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspensions.
This product seamlessly integrates with SOLIDEX™-ISOEx L Column: firstly, label cells with biotinylated primary antibodies or ligands, then add nanobeads for incubation; subsequently load the mixture suspension into the column, retain labeled cells under the magnetic field, wash to remove unlabeled cells; elute positive cells after removing the magnetic field. This method ensures minimal cell labeling, high recovery rate, suitable for immune cell sorting or removal of specific cell subsets, supporting downstream experiments such as T cell preparation or functional analysis.
Product Features
Biotin labeling can conjugate multiple antibodies
High cell purity, high recovery rate, and high cell viability
Fewer bead labeling
Gentle processing: no activation, minimal impact on downstream applications
Product Information
| Catalog No. | Product Name | Size | Price(In USD) |
|---|---|---|---|
| GMP-SMT-219-Ab01-nanoIMB-0.5mL | SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) | 0.5mL | 0.5mL |
| GMP-SMT-219-Ab01-nanoIMB-1mL | SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) | 1mL | 1mL |
| GMP-SMT-219-Ab01-nanoIMB-7.5mL | SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) | 7.5mL | 7.5mL |
Product Description
| Catalog No. | GMP-SMT-219-Ab01-nanoIMB |
| Products Name | SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based) |
| Description | SOLIDEX™ anti-Biotin Nanobeads (Column-Based) are used to enrich or deplete cells labeled with one or more biotinylated antibodies for Column-Based method |
| Format | Column-Based |
| Application | SOLIDEX™ anti-Biotin Nanobeads (Column-Free) is used to positive selection, negative selection and depletion of human and cells. |
| Storage | Store at 2-8 ℃. Do not freeze. |
Validation Data
SOLIDEX™-ISOEx Anti-Biotin Nanobeads (Column-Based) efficiently capture target cells labeled with biotinylated antibodies, enabling column-based magnetic sorting.
Catalog No. GMP-SMT-219-Ab01-nanoIMB
In this experiment, biotinylated CD3 antibody was conjugated with SOLIDEX™-ISOEx Anti-Biotin Nanobeads (Column-Based), and the labeled human PBMCs were loaded into a magnetic separation column (SOLIDEX™-ISOEx L Column) for the enrichment of CD3+ T cells. Samples before and after separation were stained with APC-CD3 antibody and analyzed by flow cytometry. Results demonstrated that the purity of CD3+ T cells increased from 52.6% before separation to 95.0% after separation. This confirms that SOLIDEX™-ISOEx Anti-Biotin Nanobeads (Column-Based), in combination with the magnetic column system, provides high capture efficiency and superior purification performance, making it suitable for positive cell selection applications requiring extremely high purity.
Fig. SOLIDEX™-ISOEx Anti-Biotin Nanobeads (Column-Based) efficiently capture target cells labeled with biotinylated antibodies.
Flow cytometry analysis showed a positive cell purity of 95.0%, demonstrating that this nanobead-column system achieves stable and reliable T cell sorting in high-throughput and high-purity scenarios.
Click to get more Data / Case study about the product.
