SOLIDEX™ CD4-T Cell Isolation Solution
Introduction of SOLIDEX™ CD4-T Cell Isolation Solution
CD4+ T cells (Helper T cells) are key regulatory cells in adaptive immunity, differentiating into subtypes such as Th1, Th2, Th17, and Treg, coordinating anti-infection responses, inflammation, and immune suppression. They play an important role in viral defense, autoimmune diseases, and transplant immunity. The primary surface marker for CD4+ T cells is CD4, which is co-expressed with CD3 (CD3+CD4+CD8-).
CD4 acts as a co-receptor, binding to MHC class II molecules, enhancing the TCR’s affinity for antigens and signal transduction, and promoting T cell activation. It is also the primary receptor for HIV entry. immunomagnetic cell separation is a commonly used technique for isolating CD4+ T cells, utilizing antibodies-coated beads for efficient separation. GeneMedi provides three types of immunomagnetic cell separation: Negative Selection/Untouched, Positive Selection, and Depletion, to meet diverse research and therapeutic needs.
Isolation method of SOLIDEX™ CD4-T Cell Isolation Solution
- Positive Selection
- Negative Selection
- Depletion
Research Applications:
Through CD4 magnetic bead enrichment, researchers can analyze T cell proliferation, cytotoxicity, cytokine production, and other functions. Positive selection is also widely used in research on viral infections, especially for evaluating the role of CD4+ T cells in immune responses by culturing and analyzing their functions in vitro.
Clinical Applications:
Positive selection has significant applications in CAR-T cell therapy. By selecting CD4+ and CD8+ T cells, researchers can prepare GD2-CAR-T cells, which showed initial success in Phase I clinical trials, with three out of four patients demonstrating significant clinical and imaging improvements. Additionally, positive selection of CD4+ and CD8+ T cells has provided a foundation for preclinical development of IL18-induced CAR-T cells targeting GD2-positive solid tumors. This method also supports large-scale automated T cell production to meet clinical demands.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T10191-iso-nanoIMB | Human CD4 Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T10191-iso-nanoIMB-CF | Human CD4 Nano-mag Beads (Column-Free) | Details |
| GM-Tg-hg-T10191-iso-rel-kit | Indirect Releasable Human CD4-T Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T10191-iso-rel-kit-CF | Indirect Releasable Human CD4-T Cell Isolation Kit (Column-Free) | Details |
| GM-Tg-hg-T10191-iso-kit | Indirect Human CD4-T Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T10191-iso-kit-CF | Indirect Human CD4-T Cell Isolation Kit (Column-Free) | Details |
Research Applications:
Negative selection removes non-CD4+ T cell populations, purifying CD4+ T cells. This method is suitable for further isolation of specific helper T cell subpopulations, such as memory CD4+ T cells and activated CD4+ T cells. These populations are crucial for studying immune responses, immune memory, and the role of T cells in antigen recognition.
Clinical Applications:
By using negative selection to enrich CD4+ T cells, researchers have successfully prepared gene-modified autologous CD4+ T cells, which were intravenously administered to patients, leading to significant improvements in immune function. The use of lentiviral vectors has shown promising results in gene transfer.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-CD4-T-Cell-iso-kit | Untouched CD4-T Cell Isolation Kit (Column-Based) | Details |
| GM-CD4-T-Cell-iso-kit-CF | Untouched CD4-T Cell Isolation Kit (Column-Free) | Details |
Research Applications:
The depletion method, which removes CD4+ T cells, is used to study the function of other immune cells without the interference of CD4+ T cells, particularly in research on tumor immune evasion, to evaluate the role of Treg cells.
Clinical Applications:
Simultaneous large-scale depletion of CD4+ and CD8+ T cells has been successfully achieved under GMP conditions, showing significant effects in adoptive immune therapy. Depleting αβ T cells and restoring functional innate effector lymphocytes further supports the potential of this treatment in immunotherapy.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T10191-dep-nanoIMB | Human CD4-T Cell Depletion Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T10191-dep-kit | Indirect Human CD4-T Cell Depletion Kit (Column-Based) | Details |
