SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) (Cat No.:GM-CD4-T-Cell-iso-kit)

Maximize your research potential with GeneMedi’s SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit. Specifically designed for Human CD4+ T Cell isolation from PBMC, this Column-based system employs a precise Untouched selection strategy.

SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is a Indirect CD4+ T Cell Isolation Kit (Column-based, Non-releasable)
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Order information


Catalog No. Product Name Size Price(In USD)
GM-CD4-T-Cell-iso-kit-10T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 10 Tests 190
GM-CD4-T-Cell-iso-kit-50T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 50 Tests 570
GM-CD4-T-Cell-iso-kit-100T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 100 Tests 1004
GM-CD4-T-Cell-iso-kit-750T SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) 750 Tests

Description


Cat No. GM-CD4-T-Cell-iso-kit
Product Name SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based)
Description SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is a Indirect CD4+ T Cell Isolation Kit (Column-based, Non-releasable)
Application SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based) is used for Negative CD4+ T Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-CD4-T-Cell-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Cocktail-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Cell population CD4-T Cell
Storage Store at 2°C–8°C

Application

  • Helper T Cell differentiation assays: Ideal for studying Th1, Th2, Th17, and Treg polarization.
  • HIV/Viral Research: High-purity CD4+ cells for studying viral entry and replication mechanisms.
  • Co-culture studies: Assessment of T cell-B cell interactions or macrophage activation.
  • Epigenetic and Transcriptomic analysis: Pristine RNA/DNA extraction for ATAC-seq or RNA-seq.

Protocol

  1. Sample Prep: Isolate PBMCs and resuspend in separation buffer.
  2. Labeling: Add the isolation cocktail to the sample and incubate for 5 minutes at room temperature.
  3. Bead Incubation: Add Anti-biotin Nanobeads and incubate for 10 minutes at 2-8°C.
  4. Separation: Apply the cell suspension to an LS Column placed in a magnetic field. Collect the flow-through, which contains the enriched, untouched CD4+ T cells.

Validation Data

Efficient Enrichment of High-Purity CD4+ T Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based)

Catalog No. GM-CD4-T-Cell-iso-kit
To evaluate the isolation efficiency of the SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based), CD4+ T cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. Non-target cells were labeled with a biotinylated antibody cocktail and depleted using magnetic beads within a separation column.
Cell fractions were stained with CD3-APC and CD4-PE for flow cytometric analysis. The frequency of CD4+ T cells demonstrated a substantial increase from 23.3% in the pre-isolation sample to 94.4% in the post-isolation fraction. These results confirm that the column-based system provides superior depletion of non-target populations, yielding highly purified "untouched" CD4+ T cells.

Figure 16. Isolation of Human CD4+ T Cells Using SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit (Column-based).Representative flow cytometry plots showing the frequency of CD4+ T cells in hPBMCs before isolation (23.3%) and after enrichment via column-based negative selection (94.4%).

ISOEx Cell Isolation Kit & Nanobeads validation
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Background

Why Choose SOLIDEX®-ISOEx Untouched Human CD4+ T Cell Isolation Kit?

  • Zero Activation: The "Untouched" negative selection ensures CD4 receptors are not engaged by antibodies, preventing artificial signaling.
  • Superior Purity: The column-based matrix enhances separation efficiency, typically achieving higher purity compared to column-free methods.
  • Rapid Workflow: Complete the labeling and separation process in approximately 20 minutes.
  • Downstream Ready: Cells are immediately ready for flow cytometry staining or culture without bead interference.

FAQ

What are the sample requirements?
This kit is optimized for use with Peripheral Blood Mononuclear Cells (PBMC). Fresh PBMCs yield the highest viability, but the kit is also compatible with cryopreserved PBMCs.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Because this is an "Untouched" (negative) selection kit, the target CD4+ T cells are free of bound antibodies or magnetic beads, leaving all surface markers available for staining.
How does the column-based negative selection work?
Non-CD4+ cells (CD8+ T cells, B cells, NK cells, Monocytes, etc.) are magnetically labeled. When passed through the column in a magnetic field, these labeled cells are retained, while the unlabeled CD4+ T cells pass through freely.
Can the columns be reused?
No. The columns are single-use consumables designed to ensure sterility and prevent cross-contamination between samples.
What is the advantage of this column-based method over column-free methods?
Column-based separation utilizes a matrix that amplifies the magnetic field, allowing for the retention of cells with very weak magnetic labeling. This often results in higher purity and recovery compared to simple tube-based (column-free) magnets.
Is this workflow compatible with automation?
Yes, the magnetic labeling and separation steps can be adapted for use with automated liquid handling systems and magnetic bead processors.