SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) (Cat No.:GM-Tg-hg-MP2610-dep-kit)

Achieve superior enrichment of untouched TCR γδT cells, NK cells, or B cells by efficiently removing Human TCR alpha-beta (abT)+ Cells from PBMC. This Depletion kit utilizes a high-performance Column-based strategy combined with indirect Magnetic Beads labeling. By effectively trapping the unwanted αβ T cells in the column, the flow-through fraction contains the target cells of interest. The result is High Purity non-TCR αβ cells with no antibody/bead residue and Zero Activation, perfectly preserving their physiological state.

SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is a Indirect TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based, Non-releasable)
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Order information


Catalog No. Product Name Size Price(In USD)
GM-Tg-hg-MP2610-dep-kit-10T SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 10 Tests 112
GM-Tg-hg-MP2610-dep-kit-50T SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 50 Tests 336
GM-Tg-hg-MP2610-dep-kit-100T SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 100 Tests 591
GM-Tg-hg-MP2610-dep-kit-750T SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) 750 Tests

Description


Cat No. GM-Tg-hg-MP2610-dep-kit
Product Name SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based)
Description SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is a Indirect TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based, Non-releasable)
Application SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit (Column-based) is used for TCR alpha-beta (abT)+ Cell Depletion, with the Key Features, such as Specific cell depletion; minimal non-specific binding; optimal for complex samples
Component for 5E8 cells (50Tests) / 1E9 cells(100tests) / 7.5E9 cells (750tests) GM-Tg-hg-MP2610-Ab-3-Biotin-50μL/250μL/500μL/3.75mL: SOLIDEX™-ISOEx Anti- Human TCR alpha-beta (abT)+ Cell monoclonal antibody(mAb) (Biotin)-50μL/250μL/500μL/3.75mL
GMP-SMT-219-Ab01-nanoIMB-100μL/0.5mL/1mL/7.5mL: SOLIDEX™-ISOEx anti-Biotin Nanobeads (Column-Based)-100μL/0.5mL/1mL/7.5mL
Labeling Type Indirect
Bead Type Non-releasable
Format Column-based
Key feature Specific cell depletion; minimal non-specific binding; optimal for complex samples
Cell population TCR+ Cell
Storage Store at 2℃-8℃

Application

  • TCR γδT Cell Research: Enrichment of untouched gamma-delta T cells for cancer immunotherapy studies.
  • GvHD Research: Depletion of TCR αβ+ cells to study Graft-versus-Host Disease reduction in allogeneic transplantation models.
  • NK Cell Analysis: Obtaining pure NK cell populations free from T cell interference for cytotoxicity assays.
  • Chimerism Analysis: Isolating specific subsets for post-transplant monitoring.

Protocol

  1. Preparation: Prepare a single-cell suspension from Human PBMCs.
  2. Labeling: Incubate cells with the primary antibody cocktail targeting TCR αβ.
  3. Bead Binding: Add the Anti-Biotin/Secondary Nanobeads to bind the primary antibodies.
  4. Separation: Apply the suspension to a column placed in a magnetic field.
  5. Collection (Depletion): The unwanted TCR αβ+ cells are retained in the column. Collect the flow-through, which contains the untouched target cells (e.g., γδT cells, NK cells).

Validation Data

Efficient depletion of TCR alpha-beta (αβ)+ T cells from human PBMC using the SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (αβ)+ Cell Depletion Kit (Column-based)

Catalog No. GM-Tg-hg-MP2610-dep-kit
This experiment was designed to evaluate the depletion efficiency of the SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (αβ)+ Cell Depletion Kit (Column-based) for removing TCR alpha-beta (αβ)+ cells. Data analysis demonstrated the kit's excellent depletion performance. In the initial PBMC sample, TCR alpha-beta (αβ)+ cells accounted for 54.7%. After processing through the separation column, the residual TCR alpha-beta (αβ)+ cells in the flow-through fraction dramatically decreased to 0.079%, indicating a depletion efficiency exceeding 99.3%. These results confirm that the kit achieves highly efficient depletion with excellent specificity, without causing non-specific loss of non-target cells.

Figure 11. Efficient depletion of TCR alpha-beta (αβ)+ cells from human PBMC using the SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (αβ)+ Cell Depletion Kit (Column-based). Results show that the proportion of TCR alpha-beta (αβ)+ cells was 54.7% before depletion (Pre-depletion) and decreased to 0.079% after depletion (After Depletion / Flow-through). This demonstrates that up to 99.3% of TCR alpha-beta (αβ)+ cells were captured and retained by the magnetic separation column, confirming the high specificity of bead capture for TCR alpha-beta (αβ)+ cells.

ISOEx Cell Isolation Kit & Nanobeads validation
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Background

Why Choose SOLIDEX™-ISOEx Indirect Human TCR alpha-beta (abT)+ Cell Depletion Kit?

  • Pristine "Untouched" Cells: The target cells in the flow-through are never bound by antibodies or beads, ensuring zero activation and native phenotype.
  • High Depletion Efficiency: The column-based high-gradient magnetic field ensures rigorous trapping of TCR αβ cells, typically removing >99% of contaminants.
  • Flexible Strategy: The indirect labeling method allows for high-sensitivity recognition of the TCR αβ complex.
  • Downstream Compatibility: Perfect for immediate flow cytometry, functional culture, or molecular analysis.

FAQ

What are the sample requirements?
This kit is optimized for fresh or cryopreserved Human PBMCs. It can also be used with Leukapheresis products. For frozen samples, ensure proper DNase treatment to prevent clumping.
Can I stain the isolated cells for Flow Cytometry immediately?
Yes. Since this is a depletion strategy, the cells in the flow-through (your target cells) have no magnetic beads or antibodies attached. Their surface markers are free and available for staining.
How does the Column-based separation work for depletion?
The column contains a ferromagnetic matrix. When placed in a magnetic field, it creates a high-gradient trap. This securely holds the magnetically labeled unwanted cells (TCR $αβ+$), preventing them from leaking into the flow-through, which ensures high purity of the non-magnetic fraction.
Are the separation columns reusable?
No. The columns are single-use to ensure sterility and prevent cross-contamination between samples.
What is the advantage of Column-based depletion over Column-free methods?
Column-based depletion generally offers higher depletion efficiency. The physical matrix traps unwanted cells more securely than simple tube-based magnetic attraction, reducing the risk of contaminating your final "untouched" sample with residual TCR αβ cells.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX™-ISOEx column format is compatible with standard automated magnetic separation instruments designed for column-based protocols.