SOLIDEX™ B Cell Isolation Solution
Introduction of SOLIDEX™ B Cell Isolation Solution
B Cells (B lymphocytes) are central to humoral immunity, responsible for producing antibodies to fight pathogens and assisting T cell activation through antigen presentation. They play a crucial role in infection defense, vaccine responses, and autoimmune diseases. Key surface markers of B cells include CD19 and CD20.
CD19 acts as a co-receptor for the B Cell Receptor (BCR), enhancing BCR signaling and promoting B cell activation, proliferation, and differentiation into plasma cells to produce antibodies. Defects in CD19 can lead to immunodeficiency disorders. immunomagnetic cell separation is a commonly used technique for isolating B cells, utilizing beads coated with antibodies for efficient separation. GeneMedi offers three types of immunomagnetic cell separation: Negative Selection/Untouched, Positive Selection, and Depletion, to meet diverse research and therapeutic needs.
Isolation method of SOLIDEX™ B Cell Isolation Solution
- Positive Selection
- Negative Selection
- Depletion
Research Applications:
Positive selection is used to isolate pure CD19+ B cells for studies on immunoglobulin production (e.g., IgE synthesis, chemotaxis), gene expression profiling, and detection of minimal residual disease in B cell malignancies, enhancing detection sensitivity.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T56365-iso-nanoIMB | Human CD19 Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T56365-iso-nanoIMB-CF | Human CD19 Nano-mag Beads (Column-Free) | Details |
| GM-Tg-hg-T56365-iso-rel-kit | Indirect Releasable Human CD19+ B Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T56365-iso-rel-kit-CF | Indirect Releasable Human CD19+ B Cell Isolation Kit (Column-Free) | Details |
| GM-Tg-hg-T56365-iso-kit | Indirect Human CD19+ B Cell Isolation Kit (Column-Based) | Details |
| GM-Tg-hg-T56365-iso-kit-CF | Indirect Human CD19+ B Cell Isolation Kit (Column-Free) | Details |
Research Applications:
Negative selection isolates untouched B cells, suitable for studies on BCR signaling, antibody secretion, vaccine responses, in vitro plasma cell differentiation, or CRISPR gene editing (e.g., BCR knockout). This method is ideal for experiments involving antibody affinity maturation. It can also be used to further isolate B cell subsets, such as analyzing transgene expression in transgenic models via Northern blot analysis.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-B-Cell-iso-kit | Untouched B Cell Isolation Kit (Column-Based) | Details |
| GM-B-Cell-iso-kit-CF | Untouched B Cell Isolation Kit (Column-Free) | Details |
Research Applications:
Depletion is used to enrich non-B cells (e.g., T cells, NK cells, with purity of 85-95%) for studies on non-B cell functions or disease models where B cells need to be reduced, such as in autoimmune diseases.
Clinical Applications:
Clinically, CD19 is often depleted in combination with CD3 or TCRαβ. For example, CD3/CD19 depletion grafts have been shown to delay T/B cell reconstitution after HHCT while accelerating NK cell recovery, enhancing antitumor activity. TCRαβ/CD19 depletion in URD HSCT is used for pediatric hematological malignancies, reducing the risk of GVHD and EBV infection.
| Cat.No | Product Name | Products Information |
|---|---|---|
| GM-Tg-hg-T56365-dep-nanoIMB | Human CD19+ B Cell Depletion Nano-mag Beads (Column-Based) | Details |
| GM-Tg-hg-T56365-dep-kit | Indirect Human CD19+ B Cell Depletion Kit (Column-Based) | Details |
