SOLIDEX®-ISOEx Magnetic Sorter-5 (Cat No.:GMP-ISOEx-Mag-S5)

Experience rapid and gentle column-free cell isolation with the SOLIDEX®-ISOEx Magnetic Sorter-5. Specifically designed to accommodate standard 5 mL flow cytometry tubes, this product generates a highly focused magnetic field. It attracts magnetically labeled cells to the tube wall, allowing for the effortless isolation of target cells or the depletion of non-target cells simply by decanting the supernatant—completely bypassing the need for sorting columns.

Caution: Extremely strong magnetic field. Keep away from computers, pacemakers, watches, and other magnetically sensitive devices.

Product Features

  • Rapid Sorting: Completes cell sorting within 25 minutes, significantly reducing experimental time.

  • User-Friendly Operation: Utilizes a simple decanting method; unlabeled cells are easily removed by gently inverting the Magnetic Sorter, eliminating the need for complex equipment.

  • High Purity and Viability: The high-strength magnetic field ensures the efficient retention of target cells, yielding an isolated cell population with exceptionally high purity and excellent viability.

  • Broad Compatibility: Beyond its seamless integration with the SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads (Column-free), it is fully compatible with mainstream magnetic beads available on the market.

  • Compact and Portable: Features a lightweight, handheld design for maximum ease of use.

  • Column-Free Design: Bypasses the need for sorting columns, streamlining the operational workflow and reducing experimental costs.


SOLIDEX®-ISOEx Magnetic Sorter-5 is developed for column-free immunomagnetic cell isolation using SOLIDEX®-ISOEx Column-Free Nanobeads, which generates a high-gradient magnetic field in the interior cavity of a standard 5 mL tube to realize the rapid and gentle isolation of labeled cells and ensure the stability and activity maintenance of cells.
Check GeneMedi's SOLIDEX®-ISOEx Cell Isolation Kit & Nanobeads


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Order information


Catalog No. Product Name Size Price(In USD)
GMP-ISOEx-Mag-S5 SOLIDEX®-ISOEx Magnetic Sorter-5 1 piece 429

Description


Cat No. GMP-ISOEx-Mag-S5
Product Name SOLIDEX®-ISOEx Magnetic Sorter-5
Description SOLIDEX®-ISOEx Magnetic Sorter-5 is developed for column-free immunomagnetic cell isolation using SOLIDEX®-ISOEx Column-Free Nanobeads, which generates a high-gradient magnetic field in the interior cavity of a standard 5 mL tube to realize the rapid and gentle isolation of labeled cells and ensure the stability and activity maintenance of cells.
Application SOLIDEX®-ISOEx Magnetic Sorter-5 is suitable for column-free negative selection of cells from small-volume samples (e.g., PBMCs, whole blood, bone marrow), enabling isolation of T cells, B cells, NK cells, monocytes, and other immune cells for downstream flow cytometry, functional studies, cell engineering, and cryopreservation.
Format Column-free
Storage Room temperature；Extremely Strong Magnetic Field. Keep away from computers, pacemakers, watches, and other objects that respond to magnetic fields.
Compatible Equipment A standard 5 mL (12 x 75 mm) polystyrene tube

FAQ

Q1: Can the SOLIDEX®-ISOEx Magnetic Sorter series be used for either positive or negative selection?
Yes, when used with our column-free nanobeads, this series fully supports both Positive Selection, Negative Selection and Depletion.
Q2: How does the isolation work?
Mechanism: After target or non-target cells are labeled with specific antibodies and magnetic particles, the sample tube is placed into the SOLIDEX®-ISOEx Magnetic Sorter. The strong magnetic field rapidly pulls the magnetically labeled cells to the wall of the tube.
  • For positive selection, the targeted cells on the wall are retained while the supernatant is poured off.
  • For negative selection, the supernatant containing the untouched target cells is collected.
Q3: Which columns do I use?
This series is entirely Column-Free. You do not need to use or purchase any column consumables.
Q4: How do I choose between Sorter-5, Sorter-15, and Sorter-50?
The choice of sorter depends strictly on your sample volume and the type of tube used:
  • SOLIDEX®-ISOEx Magnetic Sorter-5:Designed for standard 5 mL flow cytometry tubes (e.g., 12 x 75 mm tubes).
  • SOLIDEX®-ISOEx Magnetic Sorter-15:Accommodates 14 mL or 15 mL standard centrifuge tubes, ideal for medium-volume samples.
  • SOLIDEX®-ISOEx Magnetic Sorter-50:Fits 50 mL conical centrifuge tubes, specifically designed for large-volume samples or high cell concentrations.
Q5: How does the binding of the magnetic particles affect the cells?
The biological function of the cells remains completely unaltered. Our magnetic particles are biologically inert and do not induce abnormal activation or phenotypic changes upon binding.
Q6: Are the particles FACS-compatible?
Because the SOLIDEX®-ISOEx Column-Free system utilizes larger 100nm nanobeads to ensure robust magnetic capture (unlike the 50nm beads used in column-based systems), the increased particle size can interfere with the light scatter signals during flow cytometry following positive selection. Therefore, to ensure accurate FACS analysis and reliable sensitive downstream assays, we highly recommend the following:
  1. Prioritize Negative Selection:This strategy allows you to obtain "untouched," completely bead-free target cells that can be directly analyzed via FACS.
  2. Bead Removal for Positive Selection:If positive selection is required, please ensure the magnetic particles are removed from the cell surface using our dedicated release protocol prior to FACS analysis or functional assays.
Q7: Can I alter the isolation time in the magnet?
We do not recommend altering the protocol. It is strongly advised to adhere strictly to the isolation times specified in the protocol. Shortening the time leads to incomplete magnetic capture (reducing yield), while over-extending it may increase non-specific binding.
Q8: For positive selection, can I perform more than 3 isolations to increase purity?
For positive selection, 1 to 3 washes typically achieve optimal purity. While performing more than 3 washes might incrementally increase purity, it will inevitably cause mechanical stress to the cells, leading to decreased cell viability and a significant drop in overall recovery yield.
Q9: How can I analyze the purity of my enriched sample?
Purity is best analyzed via Flow Cytometry. We recommend staining the enriched cells with a fluorochrome-conjugated antibody that targets a different epitope than the one used for isolation to prevent false-negative results caused by steric hindrance.