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Package Catalog No. Price(In USD) Qty (Quantity) Sum(In USD)
1mg GMP-V-2019nCoV-Nsp3X-01-1mg 5990
10mg GMP-V-2019nCoV-Nsp3X-01-10mg 38790
100mg GMP-V-2019nCoV-Nsp3X-01-100mg 258000
≥100mg GMP-V-2019nCoV-Nsp3X-01-xmg Inquiry
Shipping Cost: 760.00
Total:



Description

Accession Number QHD43415.1
Host specics 2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Source (Expression Host)E.coli
TagN-His
Products description Recombinant 2019-nCoV(SARS-CoV-2)Nsp3-X domain(Macro domain) was expressed in E.coli-based
prokaryotic cell expression system and the target gene encoding Glu1024-Gln1198 was expressed
with 6 HIS tag at the N-terminus. The Macro domain is a module of ~180 amino acids which can bind
ADP-ribose, an NAD metabolite or related ligands.
Bioactivity validationIn Testing
PurityPurity: ≥85% (SDS-PAGE)
ApplicationImmunogen in Elisa,lateral-flow tests,and other immunoassays;Standard substance
The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass22kD
FormulationLyophilized from sterile PBS, PH 7.4
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

About Nsp3


COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2) infection. 2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-191.

Nsp3: Nsp3 (200 kDa) is the largest protein encoded by the coronavirus genome (SARS-CoV-2, SARS-CoV, MERS, etc.). Nsp3 is an essential component of the replication and transcription complex. It comprises various domains, the organization of which differs between coronavirus genera, due to duplication or absence of some domains. However, the N-terminal region of the Nsp3 is highly conserved among coronavirus, containing a ubiquitin-like (Ubl) globular fold followed by a flexible, extended acidic-domain (AC domain) rich in glutamic acid (38%). Next to the AC domain is a catalytically active ADP-ribose-1"-phosphatase (ADRP, app-1"-pase) domain (also called macro domain or X domain) thought to play a role during synthesis of viral subgenomic RNAs. SARS Unique Domain (SUD), a domain not yet identified in other coronaviruses from alphacoronavirus and betacoronavirus, follows next. The SUD domain binds oligonucleotides known to form G-quadruplexes. Downstream of the SUD domain is a second Ubl domain and the catalytically active PLpro domain that proteolytically processes the Nsp1/2, Nsp2/3 and Nsp3/4 cleavage sites1,2.

References

1. Gordon, D. E. et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. BioRxiv, doi:10.1101/2020.03.22.002386 (2020).
2. Wojdyla, J. A. et al. Papain-like protease 1 from transmissible gastroenteritis virus: crystal structure and enzymatic activity toward viral and cellular substrates. J Virol 84, 10063-10073, doi:10.1128/JVI.00898-10 (2010).





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