Recombinant 2019nCoV antigen-coronavirus
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RNA-directed RNA polymerase(RdRP, Nsp12, His Tag)
Cat No.: GMP-V-2019nCoV-RdRP001
|Package||Catalog No.||Price(In USD)||Qty (Quantity)||Sum(In USD)|
|Expression platform||2019-nCoV(SARS-CoV-2, SARS2-coronavirus)|
Recombinant 2019-nCoV(SARS-CoV-2)RNA-directed RNA polymerase (RdRP, Nsp12) was expressed in |
E.coli-based prokaryotic cell expression system and the target gene encoding Asp4891-Val5212
was expressed with His tag at the N-terminus.
|Bioactivity validation||In Testing|
|Purity||Purity: ≥95% (SDS-PAGE)|
|Application||Immunogen in Elisa,lateral-flow tests,and other immunoassays;Standard substance|
The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
|predicted Molecular Mass||39.4kD|
|Formulation||Lyophilized from sterile PBS, PH 7.4|
|Storage||Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.|
2019nCoV (SARS-CoV-2) RNA-Dependent RNA Polymerase (RdRP, Nsp12)
About Coronavirus RNA-Dependent RNA Polymerase (Coronavirus RdRP)
COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2) infection. 2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-191.
2019nCoV (SARS-CoV-2) RNA-Dependent RNA Polymerase (RdRP, Nsp12) is a multidomain RNA polymerase, which is the most conserved protein in coronaviruses. The coronavirus RNA-dependent RNA polymerase (RdRp) is essential for the viral replication and transcription.1
High-throughput screening (HTS) assay development of Coronavirus RdRP for antiviral compounds
HTS Approach: Fluorescence based assay for time-dependent kinetic analysis
1.γ-(BBT)-ATP (γ-[2'(2-benzothiazoyl)-6'-hydroxybenzothiazole)-adenosine-5'-triphosphate, Sodium salt)2,3
2. Self-priming RNA (fluorometric)2 OR 3′UTR-U30 RNA (radioactive)3
Simple 2019nCoV (SARS-CoV-2) RNA-Dependent RNA Polymerase (RdRP) activity assay develoement Protocol2,3:
2019nCoV (SARS-CoV-2) RNA-Dependent RNA Polymerase (RdRP)activity assay develoement Protocol-radioactive
2019nCoV (SARS-CoV-2) RNA-Dependent RNA Polymerase (RdRP)activity assay develoement Protocol-self priming
Figure: Screening workflow of the representative library in 384-well plates. Cip, calf intestinal alkaline phosphatase; RT, room temperature2,3.
Gordon, D. E. et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. BioRxiv, doi:10.1101/2020.03.22.002386 (2020).
2. Kocabas, F., Turan, R. D. & Aslan, G. S. Fluorometric RdRp assay with self-priming RNA. Virus Genes 50, 498-504, doi:10.1007/s11262-015-1187-8 (2015).
3. Niyomrattanakit, P. et al. A fluorescence-based alkaline phosphatase-coupled polymerase assay for identification of inhibitors of dengue virus RNA-dependent RNA polymerase. J Biomol Screen 16, 201-210, doi:10.1177/1087057110389323 (2011).