FITC antibody/antigen (BSA/OVA/KLH conjugated hapten)

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anti-FITC antibody and Carrier-coupled antigen/immunogen (hapten-carrier conjugates)

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Product information

Size： 1mg | 10mg | 100mg

Product Description

BSA-FITC

OVA-FITC

Anti-FITC mouse monoclonal antibody

Anti-FITC human monoclonal antibody

Reference

Data / case study

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Biomarker Information

1. Fluorescein Isothiocyanate (FITC): FITC, an organic compound with the chemical formula C21H11NO5S, is a remarkable fluorescent molecule that plays a central role in both scientific research and diagnostic applications. Its unique fluorescence properties make it an invaluable tool in various biopharmaceutical and in vitro diagnosis (IVD) endeavors, contributing to the precise detection, localization, and quantification of specific biomolecules.

The Role of FITC in In Vitro Diagnosis (IVD):

FITC's role in IVD encompasses a diverse range of applications, each of which leverages its distinct fluorescence characteristics to serve specific purposes:

2. Why Measure FITC?

a. Precision Biomolecule Labeling: FITC's significance in IVD begins with its ability to serve as a precision biomolecule label. Researchers and clinicians frequently employ FITC for conjugation with biomolecules, such as antibodies, proteins, nucleic acids, and peptides. This conjugation imparts a fluorescent tag, enabling the visualization and quantification of the target molecule with exceptional precision. In the context of IVD, FITC's utility is particularly evident when tracking specific analytes within clinical samples. This capability provides invaluable insights into various disease states, as well as biomarker detection and quantification.

b. Immunofluorescence Assays: Immunofluorescence assays represent a cornerstone of IVD, and FITC-conjugated antibodies are indispensable components of these assays. Immunofluorescence allows for the detection and localization of specific antigens or antibodies within biological specimens. In the context of IVD, immunofluorescence assays using FITC-labeled antibodies are vital for the diagnosis and characterization of diseases, spanning autoimmune disorders to infectious diseases. FITC's intrinsic fluorescence properties enhance the specificity and sensitivity of these assays, permitting the precise identification of target biomolecules.

c. Flow Cytometry and Cellular Analysis: Flow cytometry and cellular analysis constitute integral components of IVD. FITC-conjugated antibodies are extensively utilized in these techniques. When these antibodies bind to cell surface markers, they emit fluorescent signals, making them instrumental in cell population analysis, immunophenotyping, and the study of disease-related cellular alterations. The ability to accurately and sensitively detect and quantify specific cell populations is essential for understanding disease mechanisms and evaluating treatment outcomes.

In summary, FITC's multifaceted role in IVD encompasses its capacity for precise biomolecule labeling, facilitation of immunofluorescence assays, and empowerment of flow cytometry and cellular analysis. Measuring FITC is indispensable for achieving sensitive and accurate detection and quantification of specific biomolecules and analytes, making it an invaluable tool in biopharmaceutical research and clinical diagnostics. Its unique fluorescence properties contribute to its prominent position in the advancement of scientific knowledge and its application in improving healthcare outcomes.

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