Rickettsia conorii OmpA and OmpB antibody and antigen (recombinant protein)

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Diagnostic anti-Rickettsia conorii OmpA and OmpB antibodies pairs and antigen for animal health (animal Dog/Canine infectious disease Mediterranean Spotted Fever or Boutonneuse Fever) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P036-Tg001-Ag01	Recombinant Rickettsia conorii OmpA and OmpB protein	$3090.00
GMP-VT-P036-Tg001-Ab01	Anti-Rickettsia conorii OmpA and OmpB mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P036-Tg001-Ab02	Anti-Rickettsia conorii OmpA and OmpB mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P036-Tg001-Ab03	Anti-Rickettsia conorii OmpA and OmpB human monoclonal antibody (mAb)	$3090.00
GMP-VT-P036-Tg001-Ab04	Anti-Rickettsia conorii OmpA and OmpB human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P036-Tg001-Ag01
Product Name	Recombinant Rickettsia conorii OmpA and OmpB protein
Pathogen	Rickettsia conorii
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Rickettsia conorii OmpA and OmpB antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Rickettsia conorii level test of animal Dog/Canine infectious disease with Mediterranean Spotted Fever or Boutonneuse Fever.
Tag	His
Product description	Recombinant Rickettsia conorii OmpA and OmpB proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P036-Tg001-Ab01,GMP-VT-P036-Tg001-Ab02
Pathogen	Rickettsia conorii
Product Name	Anti-Rickettsia conorii OmpA and OmpB mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Rickettsia conorii OmpA and OmpB antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia conorii antibodies in Rickettsia conorii level test of animal Dog/Canine infectious disease with Mediterranean Spotted Fever or Boutonneuse Fever.
Product description	Anti-Rickettsia conorii OmpA and OmpB mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia conorii antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P036-Tg001-Ab03,GMP-VT-P036-Tg001-Ab04
Pathogen	Rickettsia conorii
Product Name	Anti-Rickettsia conorii OmpA and OmpB human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Rickettsia conorii OmpA and OmpB antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia conorii antibodies in Rickettsia conorii level test of animal Dog/Canine infectious disease with Mediterranean Spotted Fever or Boutonneuse Fever.
Product description	Anti-Rickettsia conorii OmpA and OmpB mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Rickettsia conorii, also known as Mediterranean spotted fever, is an obligate intracellular gram-negative bacterium belonging to the family Rickettsiaceae. It is primarily transmitted to humans through the bite of infected ticks, particularly the brown dog tick (Rhipicephalus sanguineus). This pathogen is responsible for causing Mediterranean spotted fever, a potentially serious and sometimes fatal disease characterized by a range of symptoms.

Rickettsia conorii has a small genome consisting of approximately 1.2-1.4 million base pairs, encoding fewer than 2000 genes. The genome contains genes essential for bacterial replication, metabolism, and virulence. One of the key outer membrane proteins of Rickettsia conorii is OmpA (Outer membrane protein A), which plays a vital role in host cell invasion and immune evasion. Another important surface protein is Sca (surface cell antigen), which is involved in adhesion and invasion of host cells.

When infected ticks feed on a mammalian host, Rickettsia conorii can be transmitted into the bloodstream. Within the host, the bacteria primarily target endothelial cells, which line the blood vessels. The ability of Rickettsia conorii to invade and replicate within these cells is essential for its pathogenesis. The bacterium utilizes a type IV secretion system, encoded by the virB operon, to inject effector molecules into the host cell and establish a replicative niche called the inclusion.

Mediterranean spotted fever typically presents with an incubation period of 5-7 days following the tick bite. The primary symptoms include high fever, headache, myalgia (muscle pain), and malaise. A characteristic rash often develops within a few days, starting on the extremities and spreading to the trunk. The rash is typically maculopapular, meaning it consists of flat and raised red spots. In severe cases, complications such as renal failure, respiratory distress, and neurological abnormalities can occur. Mortality rates vary, but with appropriate treatment, they are generally low.

Diagnosing Rickettsia conorii infection involves various methods. Serological tests, including enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA), detect antibodies produced in response to the bacterium. These tests can help confirm recent or past exposure to Rickettsia conorii. Molecular techniques such as polymerase chain reaction (PCR) are used to detect specific gene targets in patient samples. The citrate synthase gene (gltA) is a commonly amplified target in PCR assays for Rickettsia species. Additionally, immunohistochemistry and bacterial culture from skin biopsy specimens or blood samples may be employed for diagnostic confirmation.

The treatment of Mediterranean spotted fever typically involves the administration of antibiotics, most commonly doxycycline. Early initiation of treatment is crucial to prevent severe complications and reduce mortality. Prompt recognition and diagnosis of the disease are important factors in providing effective management.

In conclusion, Rickettsia conorii is a tick-borne bacterium that causes Mediterranean spotted fever in humans. Its ability to invade and replicate within host cells contributes to its pathogenicity. Timely diagnosis through serological tests, PCR, and other techniques is important for appropriate management and treatment. Public awareness about tick avoidance measures, such as wearing protective clothing and using insect repellents, is essential in preventing infection.

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