Rickettsia massiliae TILS antibody and antigen (recombinant protein)

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Diagnostic anti-Rickettsia massiliae TILS antibodies pairs and antigen for animal health (animal Dog/Canine infectious disease spotted fever group rickettsiae) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P037-Tg001-Ag01	Recombinant Rickettsia massiliae TILS protein	$3090.00
GMP-VT-P037-Tg001-Ab01	Anti-Rickettsia massiliae TILS mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P037-Tg001-Ab02	Anti-Rickettsia massiliae TILS mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P037-Tg001-Ab03	Anti-Rickettsia massiliae TILS human monoclonal antibody (mAb)	$3090.00
GMP-VT-P037-Tg001-Ab04	Anti-Rickettsia massiliae TILS human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P037-Tg001-Ag01
Product Name	Recombinant Rickettsia massiliae TILS protein
Pathogen	Rickettsia massiliae
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Rickettsia massiliae TILS antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Rickettsia massiliae level test of animal Dog/Canine infectious disease with spotted fever group rickettsiae.
Tag	His
Product description	Recombinant Rickettsia massiliae TILS proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P037-Tg001-Ab01,GMP-VT-P037-Tg001-Ab02
Pathogen	Rickettsia massiliae
Product Name	Anti-Rickettsia massiliae TILS mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Rickettsia massiliae TILS antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia massiliae antibodies in Rickettsia massiliae level test of animal Dog/Canine infectious disease with spotted fever group rickettsiae.
Product description	Anti-Rickettsia massiliae TILS mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia massiliae antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P037-Tg001-Ab03,GMP-VT-P037-Tg001-Ab04
Pathogen	Rickettsia massiliae
Product Name	Anti-Rickettsia massiliae TILS human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Rickettsia massiliae TILS antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Rickettsia massiliae antibodies in Rickettsia massiliae level test of animal Dog/Canine infectious disease with spotted fever group rickettsiae.
Product description	Anti-Rickettsia massiliae TILS mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Rickettsia massiliae, also known as Marseille spotted fever, is a gram-negative bacterium that falls under the family Rickettsiaceae. Belonging to the Alphaproteobacteria class, this pathogenic bacterium is classified within the order Rickettsiales.

Rickettsia massiliae is an obligate intracellular parasite, meaning it relies on host cells for survival and replication. It is primarily transmitted to humans through the bite of infected ticks, specifically those belonging to the Amblyomma genus. These ticks serve as both vectors and reservoirs for the bacteria.

With a genome size of approximately 1.3 Mb, Rickettsia massiliae contains a wide range of genes involved in various biological processes. The genome encodes around 1000 proteins, including essential ones such as outer membrane protein A (ompA), outer membrane protein B (ompB), surface cell antigens (sca), and citrate synthase (gltA).

Outer membrane protein A (ompA) plays a critical role in bacterial adhesion, host cell invasion, and evasion of host immune responses. Outer membrane protein B (ompB) is involved in the regulation of bacterial growth and metabolism, as well as interactions with host cells. Surface cell antigens (sca) are important for the formation of the bacterium's outer membrane and are implicated in the modulation of host immune responses. Citrate synthase (gltA) is a key enzyme in the citric acid cycle, which is crucial for energy production and metabolic processes within the bacterium.

When Rickettsia massiliae infects humans, it can cause a range of symptoms collectively known as spotted fever. Common clinical manifestations include high fever, headache, myalgia (muscle pain), maculopapular rash (a type of skin rash consisting of small, raised red bumps), and eschar formation (a localized skin lesion). In most cases, the symptoms are mild to moderate, but severe cases leading to hospitalization have also been reported.

Aside from humans, Rickettsia massiliae can infect various animal hosts, including dogs and cats. In these animals, the bacterium may cause similar clinical signs as seen in humans, including fever, rash, and other systemic manifestations. It is important to consider the zoonotic potential of this pathogen, as infected animals can serve as sources of transmission to humans.

To diagnose Rickettsia massiliae infection, several methods can be employed. Serological testing is commonly used to detect the presence of specific antibodies against the bacterium. This can be done through techniques like indirect immunofluorescence assays (IFA) or enzyme-linked immunosorbent assays (ELISA), targeting outer membrane proteins such as recombinant outer membrane protein A (rOmpA) and recombinant outer membrane protein B (rOmpB).

PCR-based assays are another diagnostic tool, allowing for the direct detection of Rickettsia massiliae DNA in patient samples. Primers specific to genes such as gltA, ompA, and ompB can be utilized to amplify and identify the presence of the bacterium.

In some cases, cell culture techniques may be employed to isolate and grow Rickettsia massiliae from patient samples. This method can be time-consuming and requires specialized laboratory facilities and expertise.

In summary, Rickettsia massiliae is a gram-negative bacterium belonging to the family Rickettsiaceae. Its obligate intracellular nature, transmission through ticks, and association with spotted fever make it an important pathogen to study and diagnose. Understanding its genomic features, virulence factors, and diagnostic methods aids in the development of effective strategies for prevention, control, and treatment of Rickettsia massiliae infections.

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