Bovine Coronavirus N protein antibody and antigen (recombinant protein)

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Diagnostic anti-Bovine Coronavirus N protein antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease calf enteritis, enzootic pneumonia complex and winter dysentery) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P061-Tg001-Ag01	Recombinant Bovine Coronavirus N protein protein	$3090.00
GMP-VT-P061-Tg001-Ab01	Anti-Bovine Coronavirus N protein mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P061-Tg001-Ab02	Anti-Bovine Coronavirus N protein mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P061-Tg001-Ab03	Anti-Bovine Coronavirus N protein human monoclonal antibody (mAb)	$3090.00
GMP-VT-P061-Tg001-Ab04	Anti-Bovine Coronavirus N protein human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P061-Tg001-Ag01
Product Name	Recombinant Bovine Coronavirus N protein protein
Pathogen	Bovine Coronavirus
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Bovine Coronavirus N protein antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Bovine Coronavirus level test of animal Bovines/Cattle infectious disease with calf enteritis, enzootic pneumonia complex and winter dysentery.
Tag	His
Product description	Recombinant Bovine Coronavirus N protein proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P061-Tg001-Ab01,GMP-VT-P061-Tg001-Ab02
Pathogen	Bovine Coronavirus
Product Name	Anti-Bovine Coronavirus N protein mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Bovine Coronavirus N protein antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Coronavirus antibodies in Bovine Coronavirus level test of animal Bovines/Cattle infectious disease with calf enteritis, enzootic pneumonia complex and winter dysentery.
Product description	Anti-Bovine Coronavirus N protein mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Coronavirus antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P061-Tg001-Ab03,GMP-VT-P061-Tg001-Ab04
Pathogen	Bovine Coronavirus
Product Name	Anti-Bovine Coronavirus N protein human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Bovine Coronavirus N protein antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Coronavirus antibodies in Bovine Coronavirus level test of animal Bovines/Cattle infectious disease with calf enteritis, enzootic pneumonia complex and winter dysentery.
Product description	Anti-Bovine Coronavirus N protein mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Bovine Coronavirus (BCoV) is a highly infectious pathogen that primarily affects cattle, but it can also infect other animals, including pigs and humans. The virus belongs to the family Coronaviridae, a diverse group of enveloped, positive-sense single-stranded RNA viruses that are known to cause respiratory, enteric, and systemic infections in their hosts. Other notable coronaviruses include Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV-2.

The genome of BCoV is approximately 27-32 kilobases in length and encodes several structural proteins, including the spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins. These proteins play critical roles in the viral lifecycle, from attachment and entry into host cells to assembly and release from infected cells.

The spike (S) protein of BCoV is a class I fusion protein that mediates virus attachment and entry into host cells by interacting with cellular receptors such as angiotensin-converting enzyme 2 (ACE2) and dipeptidyl peptidase 4 (DPP4). The envelope (E) protein is involved in virus assembly and release from infected cells. The membrane (M) protein is a major component of the viral envelope and plays a critical role in virus assembly, budding, and trafficking. Finally, the nucleocapsid (N) protein encapsulates the viral RNA genome and is involved in transcription and replication.

Bovine Coronavirus primarily affects cattle, but it can also infect other domesticated and wild animal species. In cattle, BCoV is known to cause winter dysentery, a highly contagious diarrheal disease often associated with severe dehydration and electrolyte imbalances. The virus is also a leading cause of neonatal calf diarrhea, which is a significant contributor to calf mortality. Other clinical signs associated with BCoV in cattle include respiratory disease and systemic infections.

In pigs, BCoV has been implicated in enteric and respiratory diseases. Porcine epidemic diarrhea (PED) caused by Porcine Epidemic Diarrhea Virus (PEDV) shares many similarities with BCoV-induced winter dysentery in cattle, including the rapid onset of diarrhea and dehydration. Like BCoV, PEDV primarily infects the cells lining the small intestine and can cause significant morbidity and mortality, particularly in neonatal piglets.

Although rare, BCoV infections have been reported in humans and are usually characterized by mild respiratory illness. In one study, BCoV was identified in the respiratory secretions of hospitalized patients with acute respiratory symptoms during an outbreak of SARS-CoV in Hong Kong in 2003, suggesting that BCoV may have contributed to the overall burden of respiratory illness during this time.

Diagnostic methods for Bovine Coronavirus include several techniques, such as serological assays, electron microscopy, virus isolation, and nucleic acid-based methods such as reverse transcription polymerase chain reaction (RT-PCR). These methods target various regions of the viral genome, including the S, E, M, and N genes, and can be used to detect and differentiate between different strains and subtypes of the virus.

Prevention and control of Bovine Coronavirus infections in cattle rely on good management practices, including strict biosecurity measures, proper hygiene, and vaccination. Several commercial vaccines are available for the prevention of winter dysentery and neonatal calf diarrhea caused by BCoV and have been shown to be effective in reducing the incidence and severity of disease. However, vaccination strategies vary depending on the target age group and production system, and additional research is needed to optimize vaccine efficacy and delivery.

In conclusion, Bovine Coronavirus is a highly infectious pathogen that primarily affects cattle but can also infect other animal species, including humans. The virus belongs to the family Coronaviridae and encodes several structural proteins that play critical roles in the viral lifecycle. Clinical signs of BCoV infections can vary depending on the host species, but they often include respiratory disease and gastrointestinal symptoms such as diarrhea and dehydration. Diagnostic methods for BCoV include several techniques, including serological assays and nucleic acid-based methods such as RT-PCR. Preventive measures such as good management practices and vaccination are crucial for the control and prevention of BCoV infections in domesticated and wild animals.

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