Bovine Rotavirus A VP7 antibody and antigen (recombinant protein)

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Diagnostic anti-Bovine Rotavirus A VP7 antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease Rotaviral diarrhoea) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P077-Tg001-Ag01	Recombinant Bovine Rotavirus A VP7 protein	$3090.00
GMP-VT-P077-Tg001-Ab01	Anti-Bovine Rotavirus A VP7 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P077-Tg001-Ab02	Anti-Bovine Rotavirus A VP7 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P077-Tg001-Ab03	Anti-Bovine Rotavirus A VP7 human monoclonal antibody (mAb)	$3090.00
GMP-VT-P077-Tg001-Ab04	Anti-Bovine Rotavirus A VP7 human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P077-Tg001-Ag01
Product Name	Recombinant Bovine Rotavirus A VP7 protein
Pathogen	Bovine Rotavirus A
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Bovine Rotavirus A VP7 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Bovine Rotavirus A level test of animal Bovines/Cattle infectious disease with Rotaviral diarrhoea.
Tag	His
Product description	Recombinant Bovine Rotavirus A VP7 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P077-Tg001-Ab01,GMP-VT-P077-Tg001-Ab02
Pathogen	Bovine Rotavirus A
Product Name	Anti-Bovine Rotavirus A VP7 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Bovine Rotavirus A VP7 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Rotavirus A antibodies in Bovine Rotavirus A level test of animal Bovines/Cattle infectious disease with Rotaviral diarrhoea.
Product description	Anti-Bovine Rotavirus A VP7 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Rotavirus A antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P077-Tg001-Ab03,GMP-VT-P077-Tg001-Ab04
Pathogen	Bovine Rotavirus A
Product Name	Anti-Bovine Rotavirus A VP7 human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Bovine Rotavirus A VP7 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Rotavirus A antibodies in Bovine Rotavirus A level test of animal Bovines/Cattle infectious disease with Rotaviral diarrhoea.
Product description	Anti-Bovine Rotavirus A VP7 mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Bovine Rotavirus A is a viral pathogen that primarily infects cattle, especially young calves. This pathogen is one of the leading causes of diarrhea in these animals, which can lead to dehydration and malnourishment, potentially resulting in death if left untreated. In this essay, we will provide a detailed account of Bovine Rotavirus A, including its structure, classification, hosts, associated diseases, and diagnostic methods.

Structure of Bovine Rotavirus A

Bovine Rotavirus A is classified as a non-enveloped virus with an icosahedral capsid, which encloses a double-stranded RNA genome, dividing into 11 coding segments. The outer capsid of the virus contains two major proteins, VP7 and VP4, responsible for viral neutralization and cellular attachment, respectively. Additionally, the core particle of the virus comprises three more significant proteins: VP1, VP2, and VP3, which help to form the polymerase complex within the host cell.

Classification of Bovine Rotavirus A

Bovine Rotavirus A belongs to the Reoviridae family, which are non-enveloped viruses that infect vertebrates and invertebrates. Reoviruses are classified on the basis of virion morphology, genome structure, and mode of replication. This pathogen is a double-stranded RNA virus with a segmented genome, and it replicates in the cytoplasm of the infected cell.

Hosts of Bovine Rotavirus A

As the name suggests, Bovine Rotavirus A mainly infects cattle, particularly young calves. It is also known to infect sheep and other domestic animals, but it rarely infects humans.

Associated Diseases Caused by Bovine Rotavirus A

Bovine Rotavirus A is one of the leading pathogens that cause diarrhea in young calves, leading to dehydration and malnourishment. The disease caused by this pathogen is highly contagious, and it can easily spread among animals in close contact. When the virus infects the small intestine, it damages the cells lining the intestinal wall, leading to reduced water and nutrient absorption. This event causes watery diarrhea and can be life-threatening for young calves, especially if left untreated.

Diagnostic methods for Bovine Rotavirus A

Several diagnostic methods are employed for detecting Bovine Rotavirus A infections. These methods include electron microscopy, enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR). Among these techniques, RT-PCR is the most reliable method for detecting Bovine Rotavirus A, as it is highly sensitive and specific. The RT-PCR technique targets specific genes and proteins, such as VP4, VP7, and NSP1 protein.

Conclusion

In conclusion, Bovine Rotavirus A is a highly contagious pathogen that mainly affects young calves and other domestic animals. It causes severe diarrhea, dehydration, and malnourishment, which can be life-threatening if left untreated. Early detection of the infection is crucial in controlling the spread of the disease among animals. Diagnostic methods, such as RT-PCR, have proven to be highly effective in the detection of Bovine Rotavirus A, providing an efficient way for veterinarians to diagnose and treat the infection. Finally, effective management of this disease may reduce the incidence of illness and mortality rate among young calves, ensuring that the animal remains healthy, productive, and protected from this viral pathogen.

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