Brucella suis antibody and antigen (recombinant protein)

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Diagnostic anti-Brucella suis antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease swine brucellosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P099-Tg001-Ag01	Recombinant Brucella suis protein	$3090.00
GMP-VT-P099-Tg001-Ab01	Anti-Brucella suis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P099-Tg001-Ab02	Anti-Brucella suis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P099-Tg001-Ab03	Anti-Brucella suis human monoclonal antibody (mAb)	$3090.00
GMP-VT-P099-Tg001-Ab04	Anti-Brucella suis human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P099-Tg001-Ag01
Product Name	Recombinant Brucella suis protein
Pathogen	Brucella suis
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Brucella suis antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Brucella suis level test of animal Swine/Porcine/Pig infectious disease with swine brucellosis.
Tag	His
Product description	Recombinant Brucella suis proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P099-Tg001-Ab01,GMP-VT-P099-Tg001-Ab02
Pathogen	Brucella suis
Product Name	Anti-Brucella suis mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Brucella suis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Brucella suis antibodies in Brucella suis level test of animal Swine/Porcine/Pig infectious disease with swine brucellosis.
Product description	Anti-Brucella suis mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Brucella suis antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P099-Tg001-Ab03,GMP-VT-P099-Tg001-Ab04
Pathogen	Brucella suis
Product Name	Anti-Brucella suis human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Brucella suis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Brucella suis antibodies in Brucella suis level test of animal Swine/Porcine/Pig infectious disease with swine brucellosis.
Product description	Anti-Brucella suis mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Brachyspira hyodysenteriae is a pathogenic bacterium that significantly impacts the swine industry by causing a disease known as swine dysentery. This condition is characterized by severe clinical symptoms in pigs, including bloody diarrhea, weight loss, and decreased feed efficiency. Understanding this pathogen, its characteristics, and diagnostic methods is essential for effective management and control of swine dysentery outbreaks.

Classification and Structure:

Brachyspira hyodysenteriae is classified as a prokaryotic bacterium and is particularly notable for its spiral or helical shape, which is characteristic of spirochetes. Spirochetes are a group of bacteria known for their unique morphology, which includes a flexible, spiral shape. This shape allows them to move actively within their host's tissues.

The outer membrane of Brachyspira hyodysenteriae contains various proteins, some of which are adhesins. Adhesins play a crucial role in the pathogenicity of the bacterium. One such protein is Hemolysin, which is involved in damaging host cells. Hemolysin can cause cell lysis, leading to tissue damage and clinical symptoms associated with swine dysentery.

Hosts and Diseases:

The primary host infected by Brachyspira hyodysenteriae is domestic pigs. Swine dysentery is a disease specific to pigs and can lead to substantial economic losses in the swine industry. Pigs of various ages can be affected, and the disease's clinical signs are usually most severe in growing and finishing pigs.

Swine dysentery manifests as mucoid and often bloody diarrhea, which is a hallmark of the disease. Affected pigs may also exhibit lethargy, decreased feed intake, weight loss, and reduced growth rates. In severe cases, the disease can lead to increased mortality, further contributing to economic losses for swine producers.

Diagnostic Methods:

Early detection of Brachyspira hyodysenteriae is crucial for preventing the spread of swine dysentery and implementing effective control measures. Several diagnostic methods are available, each with its own advantages and limitations.

Culture-Based Methods: Isolation of the bacterium can be achieved through culture-based techniques. This involves collecting fecal or intestinal samples from infected pigs and culturing them on selective agar media. Once isolated, the bacterium can be identified based on its characteristic morphology.

Serological Methods: Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination tests are examples of serological methods used to detect antibodies specific to Brachyspira hyodysenteriae in pig serum. These tests are valuable for assessing the exposure of pig populations to the bacterium.

Molecular Methods: Nucleic acid-based methods have gained importance due to their speed, specificity, and accuracy. These methods involve the detection of specific genes or genetic material associated with Brachyspira hyodysenteriae. Key genes targeted in molecular diagnostics include the 16S ribosomal RNA gene. The 16S rRNA gene is highly conserved among bacteria, making it suitable for identifying bacterial species, including Brachyspira hyodysenteriae. Additionally, other specific genes, such as those encoding Hemolysin or other virulence factors, can be targeted for diagnostic purposes.

Molecular methods are particularly valuable for their ability to detect the pathogen even in subclinical cases where pigs may be carriers without showing clinical symptoms. These carriers can contribute to disease transmission, making early detection essential for disease control.

In conclusion, Brachyspira hyodysenteriae is a pathogenic bacterium that poses a significant threat to the swine industry, causing swine dysentery characterized by bloody diarrhea and other clinical symptoms in pigs. Understanding the bacterium's characteristics and employing effective diagnostic methods, such as molecular techniques targeting the 16S rRNA gene and virulence factors, is critical for early detection and control of swine dysentery outbreaks. This knowledge is vital for minimizing economic losses and maintaining the health and productivity of pig populations.

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