Lawsonia intracellularis LsaA (tested in rabbit) antibody and antigen (recombinant protein)

 Download 

Diagnostic anti-Lawsonia intracellularis LsaA (tested in rabbit) antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease proliferative hemorrhagic enteritis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

Go to Swine/Porcine/Pig disease testing products collection >>

Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P116-Tg001-Ag01	Recombinant Lawsonia intracellularis LsaA (tested in rabbit) protein	$3090.00
GMP-VT-P116-Tg001-Ab01	Anti-Lawsonia intracellularis LsaA (tested in rabbit) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P116-Tg001-Ab02	Anti-Lawsonia intracellularis LsaA (tested in rabbit) mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P116-Tg001-Ab03	Anti-Lawsonia intracellularis LsaA (tested in rabbit) human monoclonal antibody (mAb)	$3090.00
GMP-VT-P116-Tg001-Ab04	Anti-Lawsonia intracellularis LsaA (tested in rabbit) human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P116-Tg001-Ag01
Product Name	Recombinant Lawsonia intracellularis LsaA (tested in rabbit) protein
Pathogen	Lawsonia intracellularis
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Lawsonia intracellularis LsaA (tested in rabbit) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Lawsonia intracellularis level test of animal Swine/Porcine/Pig infectious disease with proliferative hemorrhagic enteritis.
Tag	His
Product description	Recombinant Lawsonia intracellularis LsaA (tested in rabbit) proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P116-Tg001-Ab01,GMP-VT-P116-Tg001-Ab02
Pathogen	Lawsonia intracellularis
Product Name	Anti-Lawsonia intracellularis LsaA (tested in rabbit) mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Lawsonia intracellularis LsaA (tested in rabbit) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Lawsonia intracellularis antibodies in Lawsonia intracellularis level test of animal Swine/Porcine/Pig infectious disease with proliferative hemorrhagic enteritis.
Product description	Anti-Lawsonia intracellularis LsaA (tested in rabbit) mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Lawsonia intracellularis antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P116-Tg001-Ab03,GMP-VT-P116-Tg001-Ab04
Pathogen	Lawsonia intracellularis
Product Name	Anti-Lawsonia intracellularis LsaA (tested in rabbit) human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Lawsonia intracellularis LsaA (tested in rabbit) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Lawsonia intracellularis antibodies in Lawsonia intracellularis level test of animal Swine/Porcine/Pig infectious disease with proliferative hemorrhagic enteritis.
Product description	Anti-Lawsonia intracellularis LsaA (tested in rabbit) mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Pathogen Information

Lawsonia intracellularis is a pathogen that infects a wide range of domestic animal species. It is a Gram-negative, obligate intracellular bacterium that is classified under the phylum Proteobacteria and class Deltaproteobacteria. Lawsonia intracellularis is responsible for porcine proliferative enteropathy (PPE), which is characterized by thickening of the intestinal wall, diarrhea, weight loss, and reduced feed efficiency in pigs.

Lawsonia intracellularis has a genome size of approximately 1.5 Mb and encodes a variety of proteins involved in cellular processes like protein synthesis, energy metabolism, and transport. The bacterium has several genes that encode proteins for respiration, including cytochrome c oxidase and ubiquinol oxidase. These genes produce energy for the pathogen and enable it to survive inside host cells.

The main gene utilized in identifying and studying Lawsonia intracellularis is the 16S rRNA gene. This gene is commonly used in bacterial taxonomic studies and is conserved among bacteria. Other specific genes like NrdE, encoding a ribonucleotide reductase enzyme, have also been targeted in diagnostic methods.

There are several diagnostic methods used for diagnosing Lawsonia intracellularis infection. Histopathology can identify the presence of bacteria within infected cells, while immunohistochemistry uses specific antibodies to detect L. intracellularis antigens in tissue samples. Polymerase Chain Reaction (PCR) based assays can amplify and identify specific genes, including the 16S rRNA gene and other pathogenic genes such as NrdE, making it a more sensitive and specific diagnostic tool. Serological tests can also identify antibodies against Lawsonia intracellularis, but they are less specific than PCR-based assays or immunohistochemistry.

In pigs, Lawsonia intracellularis infection leads to porcine proliferative enteropathy (PPE), which is a common cause of diarrhea, poor growth performance, and mortality. In addition to being pathogenic in pigs, Lawsonia intracellularis has also been found to infect several other animal species, including horses, rabbits, hamsters, and non-human primates.

In horses, Lawsonia intracellularis infection can lead to equine proliferative enteropathy (EPE), a disease characterized by colic, weight loss, and diarrhea. In rabbits, the bacterium causes intestinal hyperplasia, which can lead to megacolon and death. Hamsters can develop intestinal lymphoma after infection with Lawsonia intracellularis. Wild rats and mice have also been found to harbor the pathogen, but its significance in these animals is not yet known.

In conclusion, Lawsonia intracellularis is a Gram-negative, obligate intracellular bacterium that infects a variety of domestic animal species and causes diseases like porcine proliferative enteropathy (PPE), equine proliferative enteropathy (EPE), and intestinal hyperplasia in rabbits and intestinal lymphoma in hamsters. Diagnostic methods include histopathology, immunohistochemistry, PCR-based assays, and serological tests, with PCR-based assays being the most sensitive and specific. Understanding the structure, classification, and diagnostic methods of pathogens like Lawsonia intracellularis is crucial for developing effective treatments and controlling the spread of diseases they cause.

About GDU