Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 antibody and antigen (recombinant protein)

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Diagnostic anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease swine pleuropneumonia) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P126-Tg001-Ag01	Recombinant Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 protein	$3090.00
GMP-VT-P126-Tg001-Ab01	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P126-Tg001-Ab02	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P126-Tg001-Ab03	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 human monoclonal antibody (mAb)	$3090.00
GMP-VT-P126-Tg001-Ab04	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 human monoclonal antibody (mAb)	$3090.00

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Product Description

Cat No.	GMP-VT-P126-Tg001-Ag01
Product Name	Recombinant Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 protein
Pathogen	Actinobacillus pleuropneumoniae serotype1, 9, 11
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Actinobacillus pleuropneumoniae serotype1, 9, 11 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Tag	His
Product description	Recombinant Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P126-Tg001-Ab01,GMP-VT-P126-Tg001-Ab02
Pathogen	Actinobacillus pleuropneumoniae serotype1, 9, 11
Product Name	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 antibodies in Actinobacillus pleuropneumoniae serotype1, 9, 11 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Product description	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P126-Tg001-Ab03,GMP-VT-P126-Tg001-Ab04
Pathogen	Actinobacillus pleuropneumoniae serotype1, 9, 11
Product Name	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 antibodies in Actinobacillus pleuropneumoniae serotype1, 9, 11 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Product description	Anti-Actinobacillus pleuropneumoniae serotype1, 9, 11 APX1 mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen Information

Actinobacillus pleuropneumoniae (APP) Serotypes 1 to 12: A Comprehensive Overview

Actinobacillus pleuropneumoniae (APP) is a pathogenic bacterium of substantial significance in the field of veterinary medicine, specifically within the swine industry. This Gram-negative, facultatively anaerobic bacterium is well-known for its involvement in porcine pleuropneumonia, a highly contagious and economically devastating respiratory disease affecting domestic pigs (Sus scrofa domesticus). Within the diverse population of Actinobacillus pleuropneumoniae, serotypes 1 to 12 play a crucial role in the manifestation of this disease.

Pathogen Classification:

APP is classified within the domain Bacteria, phylum Proteobacteria, class Gammaproteobacteria, order Pasteurellales, family Pasteurellaceae, genus Actinobacillus, and species Actinobacillus pleuropneumoniae. This classification offers insights into its phylogenetic relationships and aids in understanding its place in the microbial world.

Pathogen Structure:

The structural attributes of Actinobacillus pleuropneumoniae are essential for comprehending its pathogenicity. This bacterium possesses a complex cell envelope, consisting of lipopolysaccharides (LPS) and critical virulence factors, such as the Apx toxins.

Apx Toxins: The Apx toxins, namely ApxI, ApxII, and ApxIII, are the main virulence factors of APP. These toxins are responsible for inducing the severe lung lesions characteristic of porcine pleuropneumonia. They are encoded by specific genes located on the bacterial chromosome. Apx toxins exert their pathogenic effects by disrupting host immune defenses and causing extensive tissue damage in the porcine lung.

Lipopolysaccharides (LPS): LPS, located in the outer membrane of Actinobacillus pleuropneumoniae, contributes to the bacterium's pathogenicity by interacting with the host's immune system. LPS is a known target of the host's immune response and plays a role in evading the host's defense mechanisms.

Hosts Infected and Associated Diseases:

Actinobacillus pleuropneumoniae is highly host-specific and primarily targets domestic pigs. Infection by this bacterium results in the development of porcine pleuropneumonia. The severity of the disease can vary based on the serotype of the infecting strain and the specific virulence factors it possesses.

Porcine pleuropneumonia is characterized by a range of clinical signs, including coughing, labored breathing, fever, and pleuritis. The disease can manifest in various forms, with some cases being mild, while others can be severe and lead to significant economic losses in the swine industry. Rapid and accurate diagnosis is crucial for effective disease management and control.

Diagnostic Methods (Including Nucleic Acids):

Several diagnostic methods are employed for the detection and characterization of Actinobacillus pleuropneumoniae infections in pigs, facilitating timely intervention and control strategies. These methods include:

Bacterial Culture: Isolation and identification of the bacterium from clinical specimens, such as lung tissue or nasal swabs, through traditional microbiological techniques.

Polymerase Chain Reaction (PCR): Molecular methods, particularly PCR, are widely used for the rapid and specific detection of Actinobacillus pleuropneumoniae. Target genes commonly used in PCR assays include apxIV and apxIVA.

Enzyme-Linked Immunosorbent Assay (ELISA): ELISA tests are designed to detect antibodies produced by the host against Apx toxins. These tests help confirm exposure to the bacterium.

Immunohistochemistry: Immunohistochemical techniques are employed to identify the presence of Actinobacillus pleuropneumoniae in tissue samples, providing valuable insights into the site of infection and associated pathological changes.

Serotyping: Serotyping methods are crucial for determining the specific serotype of APP. This is achieved through the use of serotype-specific antibodies, which can aid in epidemiological studies and vaccine development.

Nucleic Acid Sequencing: Whole-genome sequencing, enabled by advances in molecular biology, offers a comprehensive genetic analysis of Actinobacillus pleuropneumoniae. This approach is particularly valuable for strain differentiation, epidemiological investigations, and understanding the genetic basis of virulence in different serotypes.

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