Clostridium perfringens beta2 antibody and antigen (recombinant protein)

 Download 

Diagnostic anti-Clostridium perfringens beta2 antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease Clostridial enterotoxicosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

Go to Multiple species disease testing products collection >>

Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P260-Tg002-Ag01	Recombinant Clostridium perfringens beta2 protein	$3090.00
GMP-VT-P260-Tg002-Ab01	Anti-Clostridium perfringens beta2 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P260-Tg002-Ab02	Anti-Clostridium perfringens beta2 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P260-Tg002-Ab03	Anti-Clostridium perfringens beta2 human monoclonal antibody (mAb)	$3090.00
GMP-VT-P260-Tg002-Ab04	Anti-Clostridium perfringens beta2 human monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P260-Tg002-Ag01
Product Name	Recombinant Clostridium perfringens beta2 protein
Pathogen	Clostridium perfringens
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Clostridium perfringens beta2 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Clostridium perfringens level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with Clostridial enterotoxicosis.
Tag	His
Product description	Recombinant Clostridium perfringens beta2 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P260-Tg002-Ab01,GMP-VT-P260-Tg002-Ab02
Pathogen	Clostridium perfringens
Product Name	Anti-Clostridium perfringens beta2 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Clostridium perfringens beta2 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium perfringens antibodies in Clostridium perfringens level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with Clostridial enterotoxicosis.
Product description	Anti-Clostridium perfringens beta2 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium perfringens antibodies.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P260-Tg002-Ab03,GMP-VT-P260-Tg002-Ab04
Pathogen	Clostridium perfringens
Product Name	Anti-Clostridium perfringens beta2 human monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	Recombinant Clostridium perfringens beta2 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium perfringens antibodies in Clostridium perfringens level test of animal Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Equine/Horse, Ovines/Sheep, Caprine/Goat, Swine/Porcine/Pig, Avian/Bird/Poultry infectious disease with Clostridial enterotoxicosis.
Product description	Anti-Clostridium perfringens beta2 mouse monoclonal antibody (mAb) is a human monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from sterile PBS, PH 7.4
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Pathogen Information

Clostridium perfringens is a pathogenic bacterium that can cause significant harm to both animals and humans. This anaerobic bacterium belongs to the phylum Firmicutes, and its morphology is characterized by rod-shaped cells and spore-forming capabilities.

The bacterium is primarily known for its ability to produce alpha-toxin using the plc gene. This potent exotoxin can cause tissue destruction and cell lysis in host organisms, leading to severe conditions such as gas gangrene, food poisoning, and necrotizing enteritis. The alpha-toxin is also known to have hemolytic properties, leading to red blood cell damage and hemolysis.

Clostridium perfringens infections are prevalent in both animals and humans, with transmission occurring through ingestion of contaminated food, exposure to wounds or injuries, inhalation of spores, or through fecal-oral transmission. In humans, the bacterium is responsible for several severe illnesses, including food poisoning, gastroenteritis, and skin infections. In animals, Clostridium perfringens causes diseases such as lamb dysentery, calf scours, and necrotic enteritis in poultry.

Several diagnostic methods are available for detecting Clostridium perfringens infections, which include culture-based assays, enzyme-linked immunosorbent assays (ELISAs), and nucleic acid-based methods. These tests utilize various genes and proteins as targets, including the 16S rRNA gene, plc gene, and cpa gene. Polymerase chain reaction (PCR)-based assays, ELISA tests, and Western blotting are among the most common diagnostic techniques employed.

Culture-based assays are the most widely used diagnostic method, and bacterial growth is usually cultured on blood agar plates under anaerobic conditions. The presence of colonies with the characteristic features of Clostridium perfringens confirms the infection. However, this method can be time-consuming and may require technical expertise.

ELISA tests and PCR-based assays have gained popularity due to their speed and high sensitivity. These methods utilize specific antibodies or primers that recognize the bacterial proteins or genes, respectively. ELISA tests detect Clostridium perfringens antigens in fecal samples or contaminated food, while PCR-based assays amplify the genetic material of the bacterium for detection.

Western blotting is another diagnostic tool for detecting the presence of Clostridium perfringens toxins in biological samples. It involves the separation of bacterial proteins by gel electrophoresis and the subsequent transfer onto a membrane. The membrane is then incubated with specific antibodies that bind to target proteins, allowing for visualization and quantification.

In conclusion, Clostridium perfringens is a Gram-positive, anaerobic bacterium capable of causing severe illnesses in both animals and humans. Its pathogenicity is mainly attributed to alpha-toxin production using the plc gene. Several diagnostic methods are available for detecting Clostridium perfringens infections, including culture-based assays, ELISA tests, PCR-based assays, and Western blotting. Rapid and accurate diagnosis is crucial for effective treatment and prevention of the spread of this potentially deadly pathogen.

About GDU