Clostridium difficile toxins (TcdA) antibody and antigen (recombinant protein)

 Download 

Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P263-Tg001-Ag01	Recombinant Clostridium difficile toxins (TcdA) protein	3090
GMP-VT-P263-Tg001-Ab01	Anti-Clostridium difficile toxins (TcdA) mouse monoclonal antibody (mAb)	1953
GMP-VT-P263-Tg001-Ab02	Anti-Clostridium difficile toxins (TcdA) mouse monoclonal antibody (mAb)	1953
GMP-VT-P263-Tg001-Ab03	Anti-Clostridium difficile toxins (TcdA) human monoclonal antibody (mAb)	1953
GMP-VT-P263-Tg001-Ab04	Anti-Clostridium difficile toxins (TcdA) human monoclonal antibody (mAb)	1953

Size： 1mg | 10mg | 100mg

Product Description

Cat No. of Products	GMP-VT-P263-Tg001-Ag01
Product Name	Recombinant Clostridium difficile toxins (TcdA) protein
Pathogen	Clostridium difficile
Target/Biomarker	toxins (TcdA)
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Clostridium difficile toxins (TcdA) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Clostridium difficile level test of animal Cat/Feline, Dog/Canine, Equine/Horse, Swine/Porcine/Pig with Clostridium difficile infection.
Tag	His
Products description	Recombinant Clostridium difficile toxins (TcdA) protein was expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-VT-P263-Tg001-Ab01, GMP-VT-P263-Tg001-Ab02
Product Name	Anti-Clostridium difficile toxins (TcdA) mouse monoclonal antibody (mAb)
Pathogen	Clostridium difficile
Target/Biomarker	toxins (TcdA)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Clostridium difficile toxins (TcdA) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium difficile antibodies in Clostridium difficile level test of animal Cat/Feline, Dog/Canine, Equine/Horse, Swine/Porcine/Pig with Clostridium difficile infection.
Tag	mFc
Products description	Anti-Clostridium difficile toxins (TcdA) mouse monoclonal antibody (mAb) is a monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-VT-P263-Tg001-Ab03, GMP-VT-P263-Tg001-Ab04
Product Name	Anti-Clostridium difficile toxins (TcdA) human monoclonal antibody (mAb)
Pathogen	Clostridium difficile
Target/Biomarker	toxins (TcdA)
Expression platform	CHO
Isotypes	Human IgG1
Bioactivity validation	Recombinant Clostridium difficile toxins (TcdA) antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Clostridium difficile antibodies in Clostridium difficile level test of animal Cat/Feline, Dog/Canine, Equine/Horse, Swine/Porcine/Pig with Clostridium difficile infection.
Tag	hFc
Products description	Anti-Clostridium difficile toxins (TcdA) human monoclonal antibody (mAb) is a monoclonal antibody produced by CHO. The antibody is ELISA validated as capture antibody and detection antibody pair.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Click to get more Data / Case study about the product.

Target/Biomarker information

Clostridium difficile: This bacterium is a very important cause of diarrhea (and more severe intestinal disease) in people, and is possibly an important cause of diarrhea in dogs and cats. It is rarely found in healthy adult pets, but is relatively common in puppies and kittens. Clostridioides difficile is a gram positive anaerob spore former. It causes diarrhoea in various species. Studies prove the incidence of C. difficile in animal food. Therefore, a zoonotic potential for humans (diarrhoea, colitis) must be implied. Additionally, there are hints of mutual transfer between dog/cat and human within a household. Most important virulence factors for the development of C. difficile infection (CDI) are the enterotoxin A (TcdA) and cytotoxin B (TcdB). Dog/cat: C. difficile can be proven in feces of healthy juvenile and adult animals as well as in animals with diarrhoea (single animals, nosocomial infections in animal hospitals and shelters). A significant correlation between C. difficile and diarrhoea could not be proven, but feces samples of animals with diarrhoea showed significantly higher TcdA (increased secretion of liquid into the intestinal lumen) and/or TcdB (lethal damage of the intestinal wall) detection as with healthy animals. Horse: Both in single animals and with diarrhoea outbreaks in herds CDI (TcdA & TcdB) occur, especially in foals, partly associated with C. perfringens, then mostly with deathly course within 3 days. Clinically indicative are colic, partly without/before diarrhoea onset and massive antibiotic associated colitis. Pig: In 1–7 days old piglets, CDI is one of the most important diarrhoea diseases (mortality up to 16%). The prevalence decreases with increasing age. The fecal-oral colonisation with C. difficile happens in endemic areas at 100% within 48h, lactogenic via the sow (ca. 25%) or aerogenic via surroundings. Clinical symptoms (yellow-watery diarrhoea, but also constipation) are not always visible. Risk factors for development of an acute CDI are age, provocation dose, but also associated toxins and the administration of antibiotics. Retarded growth, lower weaning weight and severe economical losses are the consequences. Diagnosis of an acute CDI can be difficult due to the endemic nature of C. difficile. With a two-step diagnostics of GDH (Glutamate Dehydrogenase) and the Toxins A/B, the proof can succeed with high certainty. The proof of GDH is said to be very sensitive compared to culture (golden standard) and therefore can be used as so-called “exclusion test”. On the other hand, the proof of the Toxins A/B is seen as highly specific (but less sensitive) compared to culture. Therefore, the double test can be optimally used as confirmation test.

About GDU